Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 941 results for: OMP;

Showing: 581 - 600 of 941 results

  1. Another Pathway into Cells: iTOP

    Type
    Blog Post
    ...of their luciferase assay, they concluded that components in the buffer used for protein purification must...percentages (78-79% after two rounds of transduction.) Compared to CCP transduction, iTOP is at least four times...Astolfo et al. were eager to see if iTOP would be compatible with CRISPR/Cas9 genome editing. Although CRISPR...

  2. Advice for Moving Into Sales After Your Science Postdoc

    Type
    Blog Post
    ... life science researchers. Many biotechnology companies are looking for PhD or Master’s level scientists...have done?” and “Would their academic experience compliment this sales and marketing role?”. Studying for...entrepreneurial bug and has set up an ELISA kit company on whose website you can find some great information...

  3. Pushing the Limits of DNA Assembly

    Type
    Blog Post
    .... Generation of sgRNA libraries, with maximum complexity, is achieved with synthetic oligos with a randomized... genome. Many of these techniques require more complex DNA assemblies and improvements in these methodologies...the limitations associated with otherwise more complicated assembly approaches, and opens the door for new...

  5. What Good Citizenship Can Do for Reproducibility in Science

    Type
    Blog Post
    ... It doesn’t have to be this way. We can never completely eliminate the risk that a minor variation in ...Press.  She trained originally in cell biology, completing her PhD at the MRC LMB in Cambridge UK and then... Vice President. Her current responsibilities encompass overall leadership of the Cell Press editorial...

  7. FlipGFP, a novel fluorescence protease reporter to study apoptosis

    Type
    Blog Post
    ...responsible for the degradation of over 600 cellular components ultimately leading to cell fragmentation and ...FlipGFP. FlipGFP increases fluorescence 100-fold compared to other reporters (Zhang et al., 2019). How does...and (3) β-strand 11 (β11). Each part contains components and residues that are necessary for fluorescence...

  8. Summer SciComm Series: A PhD in Science Communication

    Type
    Blog Post
    ... I asked participants to read short abstracts comparing the mortality rates between two controversial ...and biased. As a result, the control abstract comparing two fictional medical issues – chronic and acute...community. I brought along printed copies of studies comparing the filtration efficacy of different materials...

  9. Revamp Your Lab Meetings With Creative Virtual Collaboration

    Type
    Blog Post
    ...writing and this recent article published in PLOS Computational Biology (Frassl et al., 2018): “Science is increasingly...collaboratively writing a multi-authored paper. PLoS Comput Biol 14:e1006508 . https://doi.org/10.1371/journal.pcbi...Simple Rules for a Successful Collaboration. PLoS Comput Biol 3:e44 . https://doi.org/10.1371/journal.pcbi...

  10. R Bodies: Membrane-Rupturing Microscopic Tools

    Type
    Blog Post
    ...sources. While the importance of understanding these complex processes can’t be overstated, we can also learn...degradation under a variety of conditions. They are composed of 4 proteins encoded in a single operon and were...

  11. Resume Writing for Non-academic Science Careers

    Type
    Blog Post
    ...experience using image analysis software. If you use computer programs to predict protein structures, then you... you might know a thing or two about computer modeling. Make sure to focus on these skills. The only exception... into a communications role instead after she completed her MSc in Experimental Medicine. She is the Community...

  12. Plasmids 101: How to Verify Your Plasmid Using a Restriction Digest Analysis

    Type
    Blog Post
    ...than a day. Diagnostic restriction digests are comprised of 2 separate steps: 1) incubating your DNA with... construct. Consider buffer and temperature compatibility when digesting with more than one enzyme. Consult...will allow you to approximate the DNA mass of comparably intense samples of similar size. Run the gel ...

  13. Pouring LB Agar Plates

    Type
    Protocol
    ...autoclave. Pro-Tip Once your autoclave cycle is complete, we recommend opening the door to the autoclave...

  14. Western Blot

    Type
    Protocol
    ...proteins >150 kDa. Select Start Run. When the run is complete, select Done. Section 5: Blocking Prepare 1X TBST...

  15. Addgene’s Viral Service - Why Virus? Why Now?

    Type
    Blog Post
    ...service supporters We would like to thank the companies who supported this project with funding. Alnylam...donations. That seed money was used to build a completely self-supporting service so that Addgene is not...distribution, this project involved every Addgenie in the company. Our Finance, Business and Legal teams had to pave...

  17. OCaMP: A New Calcium Indicator for Neural Imaging

    Type
    Blog Post
    ...needs to emit a different color of light and be compatible with the lasers available in your microscope ...jRGECO1a and jRCaMP1a are widely used as well. To complement these tools, we recently developed a new orange...1030 nm, enabling two-photon imaging without compromising on brightness, ∆F/F0, or signal-to-noise ratio...

  18. Viral Vectors 101: Types of viruses

    Type
    Blog Post
    ...that use reverse transcriptase to synthesize complementary DNA copies of their genome upon infecting a ...gamma-retrovirus are so closely related, their required components are very similar. While plasmids containing virus-specific...between the two systems, other, more general, viral components such as a heterologous envelope or a post-transcriptional...

  19. Viral Vectors 101: Viruses as Biological Tools

    Type
    Blog Post
    ...nature, extra safety measures, like replication incompetent viral particles, are required. We will be covering...infect both non-dividing and dividing cells. They accomplish this through nuclear localization signals which... immunodeficiency virus type 1 preintegration complexes.” Proc. Natl. Acad. Sci. U.S.A. 89 (1992) 6580...

  20. Developing a cold tolerant E. coli using specialized chaperones

    Type
    Blog Post
    ...However, one of the many proteins which are not compatible with regular mesophilic systems are the psychrophilic...chaperones protein resulted in higher levels of growth compared to the E. coli strain expressing the empty vector... Medal in the Undergrad category at the iGEM Competition 2019. He has an active interest in scientific...
Showing: 581 - 600 of 941 results