We narrowed to 663 results for: PPL;

1. xCas9: Engineering a CRISPR Variant with PAM Flexibility

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   Blog Post

   ...editing rates. This PAM flexibility should expand applications that require very specific targeting, like homology...

2. Cloning Mammalian Cells with the Agarose Method

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   Blog Post

   ...population of transfected clones. The technique is applicable to a wide variety of cell lines, from HEK to ...

3. Fluorescent Proteins 101: Fluorescent Protein Timers

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   ...-FT-N1 pTRE-Slow-FT pBAD/HisB-Slow-FT -  Applications of fluorescent protein timers FP timers have...

4. Addgene's Cancer Collection Pages Connect the Research Community

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   ...any of these genes through several different applications such as fluorescent protein fusion studies and...

5. Antibodies 101: Conventional vs Spectral Flow Cytometry

   Type

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   ...order to correct for this, compensation has to be applied to “subtract” this spillover and correctly distinguish...

6. Degrading DNA with Cascade-Cas3

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   ...Mashimo, T. (2022). Genome editing technology and applications with the type I CRISPR system. Gene and Genome...

7. Pooled Library Amplifications

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   Blog Post

   ...to amplify the sample for further downstream applications. As individual libraries can vary significantly...

8. PITChing MMEJ as an Alternative Route for Gene Editing

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   ...lies in the diversity of nucleases and their applications. It will be interesting to see what new editing...

9. Of Myc and Men

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   Blog Post

   ... flexible - it could now be used in way more applications.   Fig. 1 Making a Protein Tag (Abridged...

10. Light Sheet Fluorescence Microscopy

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    Blog Post

    ...of LSFM microscopes (3,4). After this initial application, Stelzer’s group described the single-plane or...

11. Sharing Your Lab Protocols: Using Apps to Save Time & Track Your Experiments

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    ...plasmid with a downloadable protocol listed under “Supplemental Document”: gRNA_Cloning Vector (deposited by...

12. Which Fluorescent Protein Should I Use?

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    .... al. (2010) “Fluorescent proteins and their applications in imaging living cells and tissues”  Physiol...

13. Drew Endy Introduces the Biobrick Public Agreement Plasmid Collection

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    Blog Post

    ...substantial. It's estimated that genetic engineering as applied to products on the domestic market generates $300...

14. CRISPR 101: RNA Editing with Cas13

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    ...Addressing the potential use of RESCUE in therapeutic applications, the authors tested various dRanCas13b truncations...

15. New Optimized Genome-wide CRISPRko, CRISPRi, and CRISPRa Libraries

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    ...Calabrese pooled library and lentiviral prep here! Applications for the Root and Doench CRISPR libraries Overall...

16. AAVs in Retinal Gene Therapy

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    ...-1250015778 7. Yu W and Wu Z., 2018. In vivo Applications of CRISPR-Based Genome Editing in the Retina...

17. Plasmids 101: Secondary Nanobody Toolbox

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    Blog Post

    ..., Alexa Fluor 568, or Alexa Fluor 647 before application to the sample. Staining with this multi-color...

18. Better Dyeing Through Chemistry & Small Molecule Fluorophores

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    ...labels for fixed-cell imaging, not for live-cell applications. Thus, these fluorophores are often bulky and...

19. Viral Vectors 101: The Retroviral Lifecycle

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    Blog Post

    ...101: Types of Viruses Viral Vectors 101: Viral Applications Resources on addgene.org Addgene's Lentiviral...

20. Tips for Screening with Yeast Two Hybrid Systems

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    Blog Post

    ...or growth stage). A prey library can either be applied to the bait in a single population (e.g. transforming...