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We narrowed to 1,006 results for: RING

Showing: 581 - 600 of 1006 results
  1. Photosensitizer Induced Cell Ablation with FAP-TAP MG-2I-dL5**

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    Blog Post
    ...proteins or cells while having little effect on neighboring cells. The photosensitizer used in this work ...effective killing of dL5** cells without death of neighboring bystander wild-type cells.   Zebrafish experiments...photosensitizers had similar rates of off-target damage to neighboring cells. TL;DR MG-2I-dL5** is a NIR photosensitizer...
  2. Fluorescent Protein Travel Awards - FLiPs and Fluorescent Protein Biosensors

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    Blog Post
    ...hypothesized that she could make enzymes controllable by tethering them to Dronpa, a fluorescent protein known to... HMOs are thought to play an important role in nuturing the development of beneficial bacteria in the ...HMO biosensors and displayed her findings at the Spring 2017 ACS meeting. She hopes these biosensors and...
  3. Addgene Depositors Get More Citations

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    Blog Post
    .... While clearly an outlier, this story had us wondering: is there a larger trend here? Do papers associated...Management. She is particularly interested in exploring how innovative tools influence the direction of...References 1. Cong, Le, et al. "Multiplex genome engineering using CRISPR/Cas systems." Science 339.6121 (...
  4. The MTA at Addgene

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    Blog Post
    ...process. Even though this process is essential to sharing plasmids around the globe, it can be a bit of a...clear expectations from the beginning makes the sharing process smoother, more consistent, and more pleasant...MTA YouTube video for more information. Happy transferring!    Many thanks to our Tech Transfer team members...
  5. Hot Plasmids - January 2023

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    Blog Post
    ...the weaponized T-cells (CAR-T cells) - and then delivering them to a tumor - they used focused ultrasound...staining, and light exposure can negatively affect FPs during these processes. The Liu and Petsko labs recently...only brilliantly bright, but it also withstands denaturing conditions, high temperatures, and other chaotropic...
  6. With Our New Viral Service, We're Taking CRISPR Further!

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    Blog Post
    ...the Wyss Institute for Biologically Inspired Engineering and member of the Addgene Advisory board, on ...aim to alleviate this frustration by producing, titering, testing, and providing lentiviral preps of select...the Wyss Institute for Biologically Inspired Engineering and the Zhang and Doench/Root labs from the Broad...
  7. Optimizing Donor DNA for Enhanced CRISPR Genome Editing

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    Blog Post
    ...pathways can modify the sequence of a gene (thus altering its function). HDR is crucial for certain applications...Moreover, the cost of reagents is relatively modest, requiring expressed Cas9 protein, transcribed sgRNA, and...al. "Enhanced homology-directed human genome engineering by controlled timing of CRISPR/Cas9 delivery....
  8. Plasmids 101: Transformation, Transduction, Bacterial Conjugation, and Transfection

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    Blog Post
    ...until it is triggered to enter the lytic cycle.   During both of these life cycles bacterial DNA can be ...in a wild type E. coli (Griffiths et al., 2000). During conjugation, genetic material is transferred from...Guillemin. "Multiple phases of competence occur during the Helicobacter pylori growth cycle." FEMS microbiology...
  9. Plasmids 101: Screens vs. Selections

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    Blog Post
    ...approaches can be found. As an example, say you are considering either a cellular screen or a selection. A selection...scene, there are other options which are worth considering. RNAi screens are similar to CRISPR, but instead...temperature sensitive screens. These involve raising or lowering the culture temperature to exacerbate a mutant...
  10. Hot Plasmids - February 2022

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    Blog Post
    ...creates challenges for in vivo delivery and cell engineering applications. CasMINI, a new miniature CRISPR-Cas... Figure 4: Overview of CasMINI for genome engineering applications. Image from Xiaoshu, et. al. 2021...system opens up new possibilities in both cell engineering and gene therapy.  Xiaoshu X, et al. Molecular...
  11. Plasmids 101: Sequence and Ligation Independent Cloning (SLIC)

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    Blog Post
    ...ends to insert genes into plasmid backbones, base pairing between these ~4 base overhangs isn’t strong enough... be accessible to allow for complementary base pairing, so SLIC can’t be used if the overhangs would have...ligase! This post was originally written by Mary Gearing in December 2015 and was updated by Emily P. Bentley...
  12. Viral Vectors 101: Inverted Terminal Repeats

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    Blog Post
    ...an issue as they are thought to be self-repaired during the AAV replication process (Chen et al., 2004)... ITR integrity and prevent unwanted mutations?  During propagation, mutants rapidly replicate in a growth...help to reduce the likelihood of ITR deletions occurring. Check out our blog post for additional tips to...
  13. Viral Vectors 101: Types of viruses

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    Blog Post
    ...do not require breakdown of the nuclear envelope during cell division to enter the nucleus as gamma-retroviruses...AAV also has multiple serotypes (12 naturally occurring and more synthetic capsids are routinely being...lentivirus, retrovirus, adenovirus, and AAV. If you’re wondering more about what applications these viruses are...
  14. The Many Reasons for Irreproducible Research- and a Vaccine to Eradicate It

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    Blog Post
    ...these omissions, so it is unlikely to be caught during the peer review process, which often focuses more...respond as expected, use of representative images, measuring the main outcomes in several different ways, and... a particular result. Anything else that occurs during the course of the study, say a fifth time has unpredictably...
  15. Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

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    Blog Post
    ...terminator. These relatively large cassettes (considering the gRNA itself is ~100 bases) limit the number...examined editing efficiency in intact rice plants, comparing traditional gRNA constructs with PTGs. In PTG ... Mary Gearing...
  16. How to Make Friends and Meet People at a Scientific Conference

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    Blog Post
    ...of experimental problems or questions you are pondering and be ready to talk about them. People love to... to help especially when solving problems.  During the conference The conference may start in the airport...post. Stay in touch by liking, commenting and re-sharing their posts. LinkedIn users notice this sort of...
  17. Advice for Starting a Biotech Company from a Grad Student turned Entrepreneur

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    Blog Post
    ...Sample6, for advice on how to take an idea developed during graduate school and turn it into a biotech company...changed, but Sample6 still leverages the core engineering and scientific concepts to engineer bacteriophages...co-founders raised $6 million. The biggest challenge during this time was learning to communicate effectively...
  18. Human Germline Editing Using CRISPR

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    Blog Post
    ... really, and what new questions does this paper bring up? We’ll sift through the paper to understand what...results in fewer eggs. However, the potentially staggering costs of such a treatment on top of the expense... Mary Gearing...
  19. Summer SciComm Series: Cognitive Load

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    Blog Post
    ...placing on your audience when you're in the midst of sharing your science - which can drive away even the most... them restate or summarize the concepts you’re covering. Don't show frustration if they're incorrect; ...as people discuss their experiences. If you’re hearing multiple people with similar misconceptions, it...
Showing: 581 - 600 of 1006 results