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We narrowed to 1,006 results for: RING

Showing: 581 - 600 of 1006 results

  1. Quantifying DNA? Here are Five DNA Quantification Methods to Consider

    Type
    Blog Post
    ...common ways to quantify DNA. This method involves measuring the absorbance/transmission of light through a...fluorometer to quantify DNA this way. First, start by pouring your gel containing a DNA intercalating dye (ex... the run time is just a few minutes per sample. During the run, DNA fragments move through a micro- or...

  2. New and Upcoming Viral Vectors - September 2019

    Type
    Blog Post
    ...from Janelia (jGCaMP7) continue to be useful for monitoring neuronal activity in the form of calcium dynamics...fluorescence and resting fluorescence (achieved through lowering sensor resting fluorescence, while maintaining...of our viral vectors blog posts Find tips for preparing AAVs Download our viral vectors 101 eBook Resources...

  3. New and Upcoming Viral Vectors - June 2019

    Type
    Blog Post
    ...serotype for their particular study, which includes comparing the transduction patterns of different AAV serotypes...its calcium-bound state, which was achieved by lowering the basal fluorescence of the sensor. This “contrast...of our viral vectors blog posts Find tips for preparing AAVs Download our viral vectors 101 eBook Resources...

  4. The MTA at Addgene

    Type
    Blog Post
    ...process. Even though this process is essential to sharing plasmids around the globe, it can be a bit of a...clear expectations from the beginning makes the sharing process smoother, more consistent, and more pleasant...MTA YouTube video for more information. Happy transferring!    Many thanks to our Tech Transfer team members...

  5. Photosensitizer Induced Cell Ablation with FAP-TAP MG-2I-dL5**

    Type
    Blog Post
    ...proteins or cells while having little effect on neighboring cells. The photosensitizer used in this work ...effective killing of dL5** cells without death of neighboring bystander wild-type cells.   Zebrafish experiments...photosensitizers had similar rates of off-target damage to neighboring cells. TL;DR MG-2I-dL5** is a NIR photosensitizer...

  6. Fluorescent Protein Travel Awards - FLiPs and Fluorescent Protein Biosensors

    Type
    Blog Post
    ...hypothesized that she could make enzymes controllable by tethering them to Dronpa, a fluorescent protein known to... HMOs are thought to play an important role in nuturing the development of beneficial bacteria in the ...HMO biosensors and displayed her findings at the Spring 2017 ACS meeting. She hopes these biosensors and...

  7. Addgene Depositors Get More Citations

    Type
    Blog Post
    .... While clearly an outlier, this story had us wondering: is there a larger trend here? Do papers associated...Management. She is particularly interested in exploring how innovative tools influence the direction of...References 1. Cong, Le, et al. "Multiplex genome engineering using CRISPR/Cas systems." Science 339.6121 (...

  8. Hot Plasmids - January 2023

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    Blog Post
    ...the weaponized T-cells (CAR-T cells) - and then delivering them to a tumor - they used focused ultrasound...staining, and light exposure can negatively affect FPs during these processes. The Liu and Petsko labs recently...only brilliantly bright, but it also withstands denaturing conditions, high temperatures, and other chaotropic...

  9. With Our New Viral Service, We're Taking CRISPR Further!

    Type
    Blog Post
    ...the Wyss Institute for Biologically Inspired Engineering and member of the Addgene Advisory board, on ...aim to alleviate this frustration by producing, titering, testing, and providing lentiviral preps of select...the Wyss Institute for Biologically Inspired Engineering and the Zhang and Doench/Root labs from the Broad...

  10. What Good Citizenship Can Do for Reproducibility in Science

    Type
    Blog Post
    ... and understand. All of this communication and sharing fits together to make for a transparent and collaborative... for data management plans and for evidence of sharing for grant renewal—but there is clearly room to ... all its different forms as part of graduation, hiring, funding, and promotion processes, I’m confident...

  11. Optimizing Donor DNA for Enhanced CRISPR Genome Editing

    Type
    Blog Post
    ...pathways can modify the sequence of a gene (thus altering its function). HDR is crucial for certain applications...Moreover, the cost of reagents is relatively modest, requiring expressed Cas9 protein, transcribed sgRNA, and...al. "Enhanced homology-directed human genome engineering by controlled timing of CRISPR/Cas9 delivery....

  12. Fluorescent Proteins 101: Luciferase

    Type
    Blog Post
    ...resulting from the catalysis of a chemical reaction requiring luciferin, ATP and oxygen as substrates. Production... have been identified in various species with differing kinetics, substrate requirements and photon emission...these luciferases have been subjected to further engineering to improve upon the properties of the native ...

  14. The Many Reasons for Irreproducible Research- and a Vaccine to Eradicate It

    Type
    Blog Post
    ...these omissions, so it is unlikely to be caught during the peer review process, which often focuses more...respond as expected, use of representative images, measuring the main outcomes in several different ways, and... a particular result. Anything else that occurs during the course of the study, say a fifth time has unpredictably...

  15. Plasmids 101: Transformation, Transduction, Bacterial Conjugation, and Transfection

    Type
    Blog Post
    ...until it is triggered to enter the lytic cycle.   During both of these life cycles bacterial DNA can be ...in a wild type E. coli (Griffiths et al., 2000). During conjugation, genetic material is transferred from...Guillemin. "Multiple phases of competence occur during the Helicobacter pylori growth cycle." FEMS microbiology...

  16. Hot Plasmids - February 2022

    Type
    Blog Post
    ...creates challenges for in vivo delivery and cell engineering applications. CasMINI, a new miniature CRISPR-Cas... Figure 4: Overview of CasMINI for genome engineering applications. Image from Xiaoshu, et. al. 2021...system opens up new possibilities in both cell engineering and gene therapy.  Xiaoshu X, et al. Molecular...

  17. Plasmids 101: Sequence and Ligation Independent Cloning (SLIC)

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    Blog Post
    ...ends to insert genes into plasmid backbones, base pairing between these ~4 base overhangs isn’t strong enough... be accessible to allow for complementary base pairing, so SLIC can’t be used if the overhangs would have...ligase! This post was originally written by Mary Gearing in December 2015 and was updated by Emily P. Bentley...

  18. Viral Vectors 101: Types of viruses

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    Blog Post
    ...do not require breakdown of the nuclear envelope during cell division to enter the nucleus as gamma-retroviruses...AAV also has multiple serotypes (12 naturally occurring and more synthetic capsids are routinely being...lentivirus, retrovirus, adenovirus, and AAV. If you’re wondering more about what applications these viruses are...

  19. Plasmids 101: Screens vs. Selections

    Type
    Blog Post
    ...approaches can be found. As an example, say you are considering either a cellular screen or a selection. A selection...scene, there are other options which are worth considering. RNAi screens are similar to CRISPR, but instead...temperature sensitive screens. These involve raising or lowering the culture temperature to exacerbate a mutant...

  20. Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

    Type
    Blog Post
    ...terminator. These relatively large cassettes (considering the gRNA itself is ~100 bases) limit the number...examined editing efficiency in intact rice plants, comparing traditional gRNA constructs with PTGs. In PTG ... Mary Gearing...
Showing: 581 - 600 of 1006 results