We narrowed to 1,006 results for: RING;

...process. Even though this process is essential to sharing plasmids around the globe, it can be a bit of a...clear expectations from the beginning makes the sharing process smoother, more consistent, and more pleasant...MTA YouTube video for more information. Happy transferring!    Many thanks to our Tech Transfer team members...

...the weaponized T-cells (CAR-T cells) - and then delivering them to a tumor - they used focused ultrasound...staining, and light exposure can negatively affect FPs during these processes. The Liu and Petsko labs recently...only brilliantly bright, but it also withstands denaturing conditions, high temperatures, and other chaotropic...

...proteins or cells while having little effect on neighboring cells. The photosensitizer used in this work ...effective killing of dL5** cells without death of neighboring bystander wild-type cells.   Zebrafish experiments...photosensitizers had similar rates of off-target damage to neighboring cells. TL;DR MG-2I-dL5** is a NIR photosensitizer...

...hypothesized that she could make enzymes controllable by tethering them to Dronpa, a fluorescent protein known to... HMOs are thought to play an important role in nuturing the development of beneficial bacteria in the ...HMO biosensors and displayed her findings at the Spring 2017 ACS meeting. She hopes these biosensors and...

.... While clearly an outlier, this story had us wondering: is there a larger trend here? Do papers associated...Management. She is particularly interested in exploring how innovative tools influence the direction of...References 1. Cong, Le, et al. "Multiplex genome engineering using CRISPR/Cas systems." Science 339.6121 (...

...the Wyss Institute for Biologically Inspired Engineering and member of the Addgene Advisory board, on ...aim to alleviate this frustration by producing, titering, testing, and providing lentiviral preps of select...the Wyss Institute for Biologically Inspired Engineering and the Zhang and Doench/Root labs from the Broad...

...pathways can modify the sequence of a gene (thus altering its function). HDR is crucial for certain applications...Moreover, the cost of reagents is relatively modest, requiring expressed Cas9 protein, transcribed sgRNA, and...al. "Enhanced homology-directed human genome engineering by controlled timing of CRISPR/Cas9 delivery....

...until it is triggered to enter the lytic cycle.   During both of these life cycles bacterial DNA can be ...in a wild type E. coli (Griffiths et al., 2000). During conjugation, genetic material is transferred from...Guillemin. "Multiple phases of competence occur during the Helicobacter pylori growth cycle." FEMS microbiology...

...approaches can be found. As an example, say you are considering either a cellular screen or a selection. A selection...scene, there are other options which are worth considering. RNAi screens are similar to CRISPR, but instead...temperature sensitive screens. These involve raising or lowering the culture temperature to exacerbate a mutant...

...creates challenges for in vivo delivery and cell engineering applications. CasMINI, a new miniature CRISPR-Cas... Figure 4: Overview of CasMINI for genome engineering applications. Image from Xiaoshu, et. al. 2021...system opens up new possibilities in both cell engineering and gene therapy.  Xiaoshu X, et al. Molecular...

...ends to insert genes into plasmid backbones, base pairing between these ~4 base overhangs isn’t strong enough... be accessible to allow for complementary base pairing, so SLIC can’t be used if the overhangs would have...ligase! This post was originally written by Mary Gearing in December 2015 and was updated by Emily P. Bentley...

...an issue as they are thought to be self-repaired during the AAV replication process (Chen et al., 2004)... ITR integrity and prevent unwanted mutations?  During propagation, mutants rapidly replicate in a growth...help to reduce the likelihood of ITR deletions occurring. Check out our blog post for additional tips to...

...do not require breakdown of the nuclear envelope during cell division to enter the nucleus as gamma-retroviruses...AAV also has multiple serotypes (12 naturally occurring and more synthetic capsids are routinely being...lentivirus, retrovirus, adenovirus, and AAV. If you’re wondering more about what applications these viruses are...

...into our normal full plasmid NGS queue in order to bring them inline with our slower, more traditional, QC...from Ginkgo. To balance the need for speed while ensuring our rigorous QC standards are met, we are taking...’re here for and we’re glad to be able to do so during this critical time. Visit our COVID-19 Plasmids...

...these omissions, so it is unlikely to be caught during the peer review process, which often focuses more...respond as expected, use of representative images, measuring the main outcomes in several different ways, and... a particular result. Anything else that occurs during the course of the study, say a fifth time has unpredictably...

...terminator. These relatively large cassettes (considering the gRNA itself is ~100 bases) limit the number...examined editing efficiency in intact rice plants, comparing traditional gRNA constructs with PTGs. In PTG ... Mary Gearing...

...of experimental problems or questions you are pondering and be ready to talk about them. People love to... to help especially when solving problems.  During the conference The conference may start in the airport...post. Stay in touch by liking, commenting and re-sharing their posts. LinkedIn users notice this sort of...

...Sample6, for advice on how to take an idea developed during graduate school and turn it into a biotech company...changed, but Sample6 still leverages the core engineering and scientific concepts to engineer bacteriophages...co-founders raised $6 million. The biggest challenge during this time was learning to communicate effectively...

...placing on your audience when you're in the midst of sharing your science - which can drive away even the most... them restate or summarize the concepts you’re covering. Don't show frustration if they're incorrect; ...as people discuss their experiences. If you’re hearing multiple people with similar misconceptions, it...

...polyclonal antibodies include any antibodies produced during the immune response.   Polyclonal antibodies...If your protein might become slightly denatured during your experimental protocol, using a polyclonal ... (2019) Antibody Purification and Storage. Cold Spring Harb Protoc 2019:pdb.top099101. https://doi.org...