We narrowed to 728 results for: STO;

1. Antibodies 101: Polyclonal Antibodies

   Type

   Blog Post

   ...Fishman JB, Berg EA (2019) Antibody Purification and Storage. Cold Spring Harb Protoc 2019:pdb.top099101. https...

2. RNA Extraction Without A Kit

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   Blog Post

   ...sample(s) are resuspended in water or TE buffer, store them in a -80°C freezer to prevent RNA degradation...

3. Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics

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   ...dimerize, bringing each terminus of Cre together to restore Cre activity. As soon as red light or PCB is no...

4. CRISPRainbow and Genome Visualization

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   ...the 4D nucleome. Visualizing specific loci has historically been difficult, as techniques like fluorescent...

5. Plasmids 101: CcdB - The Toxic Key to Efficient Cloning

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   ...ccdB accomplish this? Let’s start with a brief history of the gene and how molecular biologists have harnessed...

6. Gendered Innovations: Why Does Sex of the Cell Matter?

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   ...blogger Londa Schiebinger, PhD, Hinds Professor of History of Science, Stanford University. Sex and gender...

7. Educational Planarian Tools for Teaching Developmental Biology Techniques

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   Blog Post

   ..., feedback etc. please write to cuttingclass@stowers.org and if you make new plasmids for investigating...

8. Lab Automation at Addgene

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   Blog Post

   ...biggest benefits of the Biomek FxP is it’s customizability. It has a large deck that can house many different...

9. Antibodies 101: Stripping and Reprobing Western Blots

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   ...applied antibodies and need to dry the membrane for storage, but plan to reprobe it later, strip it thoroughly...

10. Mouse Modeling, Part 2: Breeding and Crossing Mice

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    Blog Post

    ...colony, as opposed to ordering control mice from a stock center. This serves as a better control since the...

11. CRISPR Activators: A Comparison Between dCas9-VP64, SAM, SunTag, VPR, and More!

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    Blog Post

    ...-CBP In this method, dCas9 is fused to CBP, an histone acetyltransferase domain capable of rearranging...

12. High Complexity Golden Gate Assembly with NEB’s DAD

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    Blog Post

    ...already-existing sets. Note that this tool relies on a stochastic search algorithm rather than pre-designed sets...

13. Antibodies 101: Choosing the Right Isotype

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    Blog Post

    ...Antibody to Mouse Tissue Sections. In Journal of Histochemistry & Cytochemistry (Vol. 46, Issue 8, pp. ...

14. Corralling and Cornering the Goldilocks Antibody

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    Blog Post

    ...compatible with the desired applications (such as immunohistochemistry (IHC) or western blot), and cost-effective...

15. Split Fluorescent Proteins for Studying Protein-Protein Interactions

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    Blog Post

    ... 2in1 Kit for FRET and ratiometric BiFC from Christopher Grefen’s lab or the MoBiFC modular bimolecular...

16. BeHeard Award 2018: Diseases of Glycosylation, Arginine Mutagenesis, & Neural Development

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    Blog Post

    ... in CDG patients are still poorly studied and understood.  In line with this, Prof. Videira and collaborators...

17. Progress Towards a PAM-Free CRISPR

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    Blog Post

    ...Engineering a CRISPR Variant with PAM Flexibility History of CRISPR Cas – A Tale of Survival and Evolution...

18. Hot Plasmids February 2024

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    Blog Post

    ... inspire further-improved versions. Thanks to Christophe Leterrier, whose FocalPlanes piece on mStayGold...

19. Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

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    Blog Post

    ...plasmid that should be used to maintain a glycerol stock. Choose from: ampicillin, apramycin, blasticidin...

20. Tips for Titering Your Lentiviral Preps

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    Blog Post

    ...significantly higher than the same prep that has been stored in the freezer. If you plan to freeze a batch of...