We narrowed to 605 results for: She

1. Hot Plasmids: Summer 2024

   Type

   Blog Post

   ..., particle orientation distribution and Fourier-shell correlation curve (D), and the reconstructed 3D ...

2. CRISPR 101: Homology Directed Repair

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   Blog Post

   ...resulting in off-target effects. A technique that pushes the size limitations of ssODNs is Easi-CRISPR (...

3. Proximity Labeling: A Powerful Tool for Protein Complex Purification and Proteomic Mapping

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   Blog Post

   ...molecules per cell volume? A call to rethink some published values." Bioessays 35.12 (2013): 1050-1055. PubMed...

4. Virus Protocol - Generating Stable Cell Lines

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   Protocol

   ...deliver antibiotic resistance. This protocol was established using 293T cells but can be adapted to alternative...

5. Plasmid Cloning by PCR (with Protocols)

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   Protocol

   ...amount of recipient plasmid to insert. Isolate the Finished Plasmid Finally, you will need to pick individual...

6. Generating Mouse Models Using CRISPR/Cas9

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   ...world, I (Wenning) was fascinated by CRISPR and rushed to test it. I still remember the day, March 16,...

7. Plasmids 101: Restriction Cloning

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   Blog Post

   ...ratio” in the ligation reaction. 5. Purify the finished plasmid   Once it looks like your ligation has...

8. CRISPR 101: Epigenetics and Editing the Epigenome

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   ...Originally published Feb 14, 2017 and updated Jun 24, 2020. Epigenetic modifications are an additional...

9. Easi-CRISPR: Generating Knock-In and Conditional Mouse Models

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   ...PAM (bold highlighted text) and cleavage sites (slashes) are shown. In this case, the green guide would...

10. Antibodies 101: Multiplex Immunofluorescence

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    Blog Post

    ...the secondary antibody, it should get completely washed away and leave no signal. If you see lots of extra...

11. Quick Guide to All Things Lentivirus

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    Blog Post

    ... generations of lentiviral vectors have been established so far, with safety increasing in each generation...

12. RNA Interference in Plant Biology: New Tools for an Old Favorite

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    ...837597 Li J-F, Chung HS, Niu Y, Bush J, McCormack M, Sheen J (2013) Comprehensive Protein-Based Artificial ...

13. When Fidelity Matters: A frank discussion about ligase fidelity

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    Blog Post

    ...New England Biolabs® (NEB) researchers recently published a method for the high-throughput profiling of ...

14. AAV ddPCR Titration

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    Protocol

    ...dUTP, Bio-Rad,1863023 10% Poloxamer 188, Thermo Fisher, 24040032 Droplet generation oil, Bio-Rad, 1863005...

15. Custom CRISPR Screens & the Green Listed Software

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    Blog Post

    ...like to use in your screen. Currently, eleven published libraries are embedded. These were kindly donated...

16. Antibodies 101: Introduction to Antibodies

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    Blog Post

    ...out of the solution. Once the unbound protein is washed away, the protein of interest is dissociated from...

17. Hassle-free 96-well Format Epitope Tagging Using Cas9 Ribonucleoprotein

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    Blog Post

    ... tagging is scalable to 96-well format. Off-the-shelf CRISPR reagents for the knock-in experiment were...

18. Antibodies 101: Flow Cytometry

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    ...first resuspended in a pressurized buffer called sheath fluid and transported through tubes or capillaries...

19. Typing CRISPR Systems

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    Blog Post

    ... Alyssa Shepard...

20. A Primer on Optogenetics: Introduction and Opsin Delivery

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    Blog Post

    ...the many primary research papers that have been published at this point.  In brief, some standard opsins...