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...case, however, and recent work has already been published on improving base editing precision. Similar treatments...

... Alyssa Shepard...

...resulting in off-target effects. A technique that pushes the size limitations of ssODNs is Easi-CRISPR (...

...deliver antibiotic resistance. This protocol was established using 293T cells but can be adapted to alternative...

...PAM (bold highlighted text) and cleavage sites (slashes) are shown. In this case, the green guide would...

...Originally published Feb 14, 2017 and updated Jun 24, 2020. Epigenetic modifications are an additional...

...world, I (Wenning) was fascinated by CRISPR and rushed to test it. I still remember the day, March 16,...

...ratio” in the ligation reaction. 5. Purify the finished plasmid   Once it looks like your ligation has...

...the secondary antibody, it should get completely washed away and leave no signal. If you see lots of extra...

...amount of recipient plasmid to insert. Isolate the Finished Plasmid Finally, you will need to pick individual...

... generations of lentiviral vectors have been established so far, with safety increasing in each generation...

...New England Biolabs® (NEB) researchers recently published a method for the high-throughput profiling of ...

...837597 Li J-F, Chung HS, Niu Y, Bush J, McCormack M, Sheen J (2013) Comprehensive Protein-Based Artificial ...

...dUTP, Bio-Rad,1863023 10% Poloxamer 188, Thermo Fisher, 24040032 Droplet generation oil, Bio-Rad, 1863005...

...like to use in your screen. Currently, eleven published libraries are embedded. These were kindly donated...

... tagging is scalable to 96-well format. Off-the-shelf CRISPR reagents for the knock-in experiment were...

...out of the solution. Once the unbound protein is washed away, the protein of interest is dissociated from...

...first resuspended in a pressurized buffer called sheath fluid and transported through tubes or capillaries...

... Alyssa Shepard...

...the many primary research papers that have been published at this point.  In brief, some standard opsins...