We narrowed to 888 results for: TRI

... libraries are typically DNA preps, but we do distribute several of our most popular CRISPR pooled libraries...cells at a concentrated seeding density while centrifuging. Spinfection transduction efficiency can vary...

...the host organism being unable to synthesize a nutrient required for growth. Before delivery of the vector...cells are grown in media that supplies the key nutrient. The vector provides the missing synthesis machinery...

...This post was contributed by Samuel Mortensen, a PhD candidate at Northeastern University. Working with... 5.02 (2003): 103-115. Hu, Ching-yeh, et al. "Intrinsic GUS-like activities in seed plants." Plant cell...

...on to learn how the design of PHP.B may have contributed to this mouse-specific performance, why LY6A ...selection for PHP.B in C57BL/6J mouse strains likely contributed to the unintentionally selection of a virus with...

...This post was contributed by Erik Snapp, the Director of Student and Postdoctoral Programs at the Janelia...mechanistic, doable by 1-3 people in 1-5 years, and contribute to resolving the overarching knowledge gap. Aims...

... quick chat can be rather tricky (think people based in different countries). Here social media is a connector...

...start answering questions. r/Science has pretty strict rules about not being too sales-y in posts which...term “implicit bias” which is apparently a troll trigger phrase these days). Redditors ask serious questions...

...vial of the desired cell line from a reputable distributor such as the American Type Culture Collection ...cloudy. Mycoplasma compete with host cells for nutrients and can alter expression of receptors, ion channels...

...Example steps include: permeabilization, antigen retrieval, and sectioning. Blocking Block is used to...validated antibodies? Many companies that produce and distribute antibodies validate them in a variety of applications...

...This post was contributed by guest blogger Daria Shcherbakova, a faculty member at Albert Einstein College...Shemetov et al., 2021). In addition to FRET, intensiometric calcium indicators NIR-GECOs were developed...

...doing so, they identified the nucleotides that contribute to the instability of Spinach, and were able ...superfolding Spinach2 reveals the dynamic nature of trinucleotide repeat–containing RNA." Nature methods 10.12...

...Molecular and Cellular Biology, harnessed these attributes of Cre-lox to create the Brainbow mouse neural...homeostasis results from neutral competition between symmetrically dividing Lgr5 stem cells. Snippert HJ, van der...

....89.12.5547. PMID: 1319065; PMCID: PMC49329. Triezenberg SJ, Kingsbury RC, McKnight SL. Functional dissection...Park, S., Hill, C. et al. A LexAop > UAS > QUAS trimeric plasmid to generate inducible and interconvertible...

...sulfate proteoglycan (HSPG)3 AAV receptor (AAVR; not strictly required)2 Human fibroblast growth factor ...to Addgenies Brian O’Neill and Ina Ersing for contributing their expertise! This post was originally published...

... activated tools, can control everything from electrical activity to gene expression, and even CRISPR ...2019). Cortical layer–specific critical dynamics triggering perception. Science, 365(6453), eaaw5202. https...

...This post was contributed by Steph Guerra, a AAAS Science and Technology Policy Fellow at the Veterans... challenging if you are trying to apply for an outright job in a field where you have no specific scientific...

... that almost all off-target expression can be attributed to spontaneous recombination between homologous...the amount of leaky expression can largely be attributed to the level of spontaneous recombination between...

...This post was contributed by Erik Snapp, the Director of Student and Postdoctoral Programs at theJanelia... your time as they can result in spurious yet intriguing findings that fail to replicate. Plan experiments...

...all know that in the lab there are often little tricks that are essential for experiments but that nobody... not (Figure 4). 5. In theory you could use densitometric analysis to quantitate the intensity of the ...

...your cells. While proteins themselves can emit intrinsic fluorescence when excited with ultraviolet (UV...use a dimmer fluorophore. However, these aren't strict rules and in practice the differences in brightness...