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We narrowed to 897 results for: TRI

Showing: 581 - 600 of 897 results
  1. Cpf1: A New Tool for CRISPR Genome Editing

    Type
    Blog Post
    ...functions differently. Since Cpf1 loci are widely distributed across bacterial species, Zetsche et al. hypothesized...directional gene transfer, analogous to traditional restriction enzyme cloning. Sticky-end mediated gene transfer...
  2. A Career in Grants: How To Become a Grant Professional

    Type
    Blog Post
    ... This post was contributed by Jo Miller, founder and principal of J. Miller & Associates, Inc., a national...professionals is learning to succeed in grants through trial and error, through winning and losing funding, and...
  3. Negotiating Work and Life: How to Find the Joy

    Type
    Blog Post
    ...an elderly family member, want to train for a triathlon, finally buy that charming fixer-upper house you... every school performance, chaperone every field trip or cheer at every soccer game. Tip: take turns going...
  4. Save Time with Transient Plant Leaf Transformations

    Type
    Blog Post
    ...This post was contributed by Samuel Mortensen, a PhD candidate at Northeastern University. Working with... 5.02 (2003): 103-115. Hu, Ching-yeh, et al. "Intrinsic GUS-like activities in seed plants." Plant cell...
  5. To Codon Optimize or Not: That is the Question

    Type
    Blog Post
    ...changes to a gene sequence can cause unexpected detrimental results to the protein. Codon optimization could...ZE, Kimchi-Sarfaty C (2011) Understanding the contribution of synonymous mutations to human disease. Nat...
  6. Transferable Skills Guide: Creativity

    Type
    Blog Post
    ...kill your productivity. Harvard Business Review. Retrieved January 14, 2022, from https://hbr.org/2019/11...innovation on your team. Harvard Business Review. Retrieved January 14, 2022, from https://hbr.org/2019/07...
  7. Plasmids 101: Shuttle Vectors

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    Blog Post
    ...the host organism being unable to synthesize a nutrient required for growth. Before delivery of the vector...cells are grown in media that supplies the key nutrient. The vector provides the missing synthesis machinery...
  8. Designing Your Chalk Talk for the Academic Job Interview

    Type
    Blog Post
    ...This post was contributed by Erik Snapp, the Director of Student and Postdoctoral Programs at the Janelia...mechanistic, doable by 1-3 people in 1-5 years, and contribute to resolving the overarching knowledge gap. Aims...
  9. How to Be an Excellent Trainee

    Type
    Blog Post
    ...than any other one. Luckily, you can do a lot to contribute to the relationship dynamic and set yourself ...notes on general protocol steps and other tips and tricks your mentor shares. Additionally, go ahead and ...
  10. Viral Vectors 101: Preparing Pooled Libraries

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    Blog Post
    ... libraries are typically DNA preps, but we do distribute several of our most popular CRISPR pooled libraries...cells at a concentrated seeding density while centrifuging. Spinfection transduction efficiency can vary...
  11. 5 Reasons to Use Reddit for Science Communication

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    Blog Post
    ...start answering questions. r/Science has pretty strict rules about not being too sales-y in posts which...term “implicit bias” which is apparently a troll trigger phrase these days). Redditors ask serious questions...
  12. Getting the Most from Your Lentiviral Transduction

    Type
    Blog Post
    ...vial of the desired cell line from a reputable distributor such as the American Type Culture Collection ...cloudy. Mycoplasma compete with host cells for nutrients and can alter expression of receptors, ion channels...
  13. Antibodies 101: Introduction to Immunofluorescence

    Type
    Blog Post
    ...Example steps include: permeabilization, antigen retrieval, and sectioning. Blocking Block is used to...validated antibodies? Many companies that produce and distribute antibodies validate them in a variety of applications...
  14. Quick Guide to Near-Infrared Fluorescent Proteins

    Type
    Blog Post
    ...This post was contributed by guest blogger Daria Shcherbakova, a faculty member at Albert Einstein College...Shemetov et al., 2021). In addition to FRET, intensiometric calcium indicators NIR-GECOs were developed...
  15. The Fluorescent Vegetables in Aptamer Soup

    Type
    Blog Post
    ...doing so, they identified the nucleotides that contribute to the instability of Spinach, and were able ...superfolding Spinach2 reveals the dynamic nature of trinucleotide repeat–containing RNA." Nature methods 10.12...
Showing: 581 - 600 of 897 results