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  3. Plasmids 101: Mammalian Vectors

    Type
    Blog Post
    ...components greatly reduces the rate of plasmid dilution but does not guarantee 100% transfection efficiency...

  4. AAV Titration by qPCR Using SYBR Green Technology

    Type
    Protocol
    ...of 2 x 10 8 dilution 90 2 x 10 7 10 of 2 x 10 7 dilution 90 2 x 10 6 10 of 2 x 10 6 dilution 90 2 x 10 ...Total dilution Dilution 1 (DNase step) 5 uL AAV stock 45 uL 10X 10X Dilution 2 5 uL Dil. 1 95 uL 20X 200X...200X Dilution 3 20 uL Dil. 2 80 uL 5X 1000X Dilution 4 20 uL Dil. 3 80 uL 5X 5000X Dilution 5 20 uL Dil...25000X Dilution 6 20 uL Dil. 5 80 uL 5X 125000X Dilution 7 20 uL Dil. 6 80 uL 5X 625000X Dilution 8 20 ... To obtain a solution at 2 x 10 9 molecules/μL: 1.59 x 10 11 / 2 x 10 9 = 79.8X dilution ...10 5 10 of 2 x 10 5 dilution 90 2 x 10 4 10 of 2 x 10 4 dilution 90 2 x 10 3 Pro-Tip To help stabilize ... standard dilution. Dilute DNase-treated and AAV reference samples according to the dilution scheme in...

  6. Lentivirus ddPCR Titration

    Type
    Protocol
    ...transfer 200 µL of the first dilution into the next dilution. Repeat for all dilutions as indicated in the chart... virus dilutions were used to transduce cells: Initial dilution factor of virus stock Dilution factor ... µL 5 200 µL of 2.5-fold dilution 200 µL 400 µL 10 200 µL of 5-fold dilution 200 µL 400 µL 20 200 µL of... of 10-fold dilution 200 µL 400 µL 40 200 µL of 20-fold dilution 200 µL 400 µL 80 200 µL of 40-fold dilution... 160 200 µL of 80-fold dilution 200 µL 400 µL Add 150 µL of each viral dilution to a well of a 6-well ... viral dilution). Remember, this is an additional 10-fold dilution of the virus stock! Don’t forget to...~2 as you progress from the higher dilutions to the lower dilutions (blue). As shown in the image (Figure...

  7. Handling Plasmids from Addgene - Purifying Plasmid DNA

    Type
    Protocol
    ...EDTA Store Solution I at 4°C Solution II - Denaturing Solution 0.2 N NaOH 1.0% SDS Store Solution II at room...plasmid Resuspension buffer Denaturing solution Renaturing solution 2 mg/mL RNase A TE or water-saturated...that is compatible with the next solution. Add a denaturing solution to the resuspended bacteria. Note...contents, including plasmid DNA, into solution. Add a renaturing solution to the denatured bacteria. Note: ...Lysis Plasmid Miniprep Prepare the following solutions: Solution I - Resuspension Buffer 25 mM Tris-HCl (pH... room temperature Solution III - Renaturing Solution (Potassium Acetate) 120 mL 5M Potassium acetate 23...Resuspend the pellet in 100 μL of cold Solution I. Vortex the solution for 2 min or until all bacteria are...

  10. Hot Plasmids - February 2022

    Type
    Blog Post
    ...requirements (Figure 1), but Cas12a has generally been underutilized in genetic screens because of its lowered editing...

  12. Antibodies 101: Isotypes

    Type
    Blog Post
    ...edition. New York: Garland Science; 2001. The distribution and functions of immunoglobulin isotypes. Available...

  14. Clear and Accessible Writing

    Type
    Blog Post
    ...macromolecule density is equal to the buoyant force of the solution. Contaminants of lower densities are captured ...

  18. Plasmids 101: Inducible Promoters

    Type
    Blog Post
    ...promoters, are always active and thus referred to as constitutive promoters. Others are only active under specific...

  19. A Deep Dive into BioBuilder

    Type
    Blog Post
    ...biotechnology.” These are the types of inquiries that routinely hit the inbox at the BioBuilder Educational Foundation...
Showing: 581 - 600 of 739 results