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We narrowed to 746 results for: UTY

Showing: 581 - 600 of 746 results

  3. Plasmids 101: Mammalian Vectors

    Type
    Blog Post
    ...components greatly reduces the rate of plasmid dilution but does not guarantee 100% transfection efficiency...

  5. Antibody Validation Using the Indirect ELISA Method

    Type
    Protocol
    ...different incubation options available. Caution Use the Stop Solution in a well ventilated area and avoid ...Immediately before use, mix 5 mL TMB Solution and 5 mL Peroxide Solution from the TMB substrate kit. Cap the...determine the ideal concentration. Prepare serial dilutions of the purified antigen as follows: 2 ng/µL : ... wells of each row, using a different standard solution for each row of a 96-well ELISA microplate. Cover...the wash buffer from the wells. Prepare antibody dilution buffer (0.1% BSA in PBS) as follows: Dilute 50... to the desired concentrations in the antibody dilution buffer. Pro-Tip The ideal antibody concentration...multichannel pipette, add 100 µL of primary antibody solution to the appropriate wells. Cover the plate with...

  8. Plasmids 101: Inducible Promoters

    Type
    Blog Post
    ...promoters, are always active and thus referred to as constitutive promoters. Others are only active under specific...

  9. A Deep Dive into BioBuilder

    Type
    Blog Post
    ...biotechnology.” These are the types of inquiries that routinely hit the inbox at the BioBuilder Educational Foundation...

  12. Hot Plasmids - February 2022

    Type
    Blog Post
    ...requirements (Figure 1), but Cas12a has generally been underutilized in genetic screens because of its lowered editing...

  14. Antibodies 101: Isotypes

    Type
    Blog Post
    ...edition. New York: Garland Science; 2001. The distribution and functions of immunoglobulin isotypes. Available...

  17. Clear and Accessible Writing

    Type
    Blog Post
    ...macromolecule density is equal to the buoyant force of the solution. Contaminants of lower densities are captured ...
Showing: 581 - 600 of 746 results