We narrowed to 835 results for: ang

...for researchers looking to generate long-lasting changes in cells. However, for some applications, integration...construct has the D64V mutation, a single amino acid change (from aspartic acid to valine at position 64) which...

...techs’ financial books.  To find personnel salary ranges, go to online job information sites (like, indeed.com...focused on research relevant objectives and their tangible resources. For example, if a dean/chair asks “...

...palette for display (like green-magenta-blue or orange-blue rather than red-green). Even better, show ..., Ferling, I., Gazda, M. A., Hoeppner, L. H., Ilangovan, V., Lo, H., Olson, M., Mohamed, S. Y., Sarabipour...

... a wide variety of genomic edits — single-base changes, small deletions, and large insertions up to 12... B., Tran, T., … Dimidschstein, J., Fishell, G., Wang, Y. (2024). An enhancer-AAV toolbox to target and...

...s chromatin structure undergoes significant rearrangement (such as when a stem cell initiates differentiation...researchers to avoid making unnecessary base pair changes as would be the case with ssODNs. One minor drawback...

...Make one yourself. Instead of thinking “What’s my angle on attacking this problem?”, try thinking “Who can...can I assemble to attack this problem from every angle?” Your science will be better for it. Additional...

...retroviral vectors because they target a broader range of cells and their integration into the genome is...you put in your culture. As viral production can change from one batch to another. For experimental consistency...

...McClinntock who first discovered transposons due to changes in coloration of corn kernels. Aside from corn,... et al., 2021). Target site preference and host range are important to consider when selecting a transposon...

...service (Lee et al., 2015). This kit offers a broad range of parts, including copy number machinery, chromosomal...elements such as promoters or fluorophores are interchangeable across organisms. When using the MK system,...

...fresh media containing selection reagent Day 3–14: Change media as needed Day 14–18: Expand and harvest stable...cell line. To do this, treat target cells with a range of doses of antibiotic and determine the lowest ...lentiviral aliquot on ice prior to use. Prepare a range of dilutions of the lentivirus in DMEM complete ...titered your virus beforehand, you can narrow this range according to the results of your titration. Mix ...lentivirus, above) are dying. Perform regular media changes and monitor the growth of the cells. Pro-Tips Depending...culture, so it is important to do regular media changes and maintain optimal growth conditions for the ...absence of cell death, the cell media should be changed every 2–3 days to maintain the dose of antibiotic...

...Option 2 with a buffer exchange/concentration using an Amicon 30 kDa MWCO buffer exchange column. (Optional... Day 1: Purify antibody Day 2 or later: Buffer exchange Equipment Class II, Type A2 Biological Safety ...above 1.0 mg/mL proceed to Option 1 with a buffer exchange using a Zeba Spin 7 kDa MWCO desalting column....the same recombinant antibody. Section 2: Buffer exchange Choose Option 1 or Option 2 based on the concentration...concentration of the pooled sample above. Option 1: Buffer exchange using a desalting column The 10 mL Zeba Spin Desalting...sterile sodium azide to 1 mM. Option 2: Buffer exchange and concentration with an Amicon column Apply ...least 4 additional times to ensure a full buffer exchange. Discard the flow through into a waste container...

... Jennifer Tsang...

... Jennifer Tsang...

... to use CRISPR plasmids in the lab and the vast range of possibilities at their disposal. With this update...

... Jennifer Tsang...

...Hot Plasmid 3: CRISPR base editors with enhanced range and specificity. Listen to to the Segmennt of Improved...

...gradual move off X as a response to major platform changes, such as limiting access and increased priority...

... the authors Patrick Hsu, Eric Lander, and Feng Zhang acknowledge the role of data and materials repositories...

... Jennifer Tsang...

...Phuels lab deposited a plasmid that will forever change the fly lab experience. The Frequency Light Interval...