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  1. Plasmids 101: Control Plasmids

    Type
    Blog Post
    ... are many, many different types of experiments carried out by scientists every day. Although the designs...
  2. Protocol - How to Inoculate a Bacterial Culture

    Type
    Protocol
    ...Inoculating a Liquid Bacterial Culture Plasmids can carry one or more antibiotic resistance genes, which confer...resistance to a specific antibiotic to the bacteria carrying them. The presence of an antibiotic resistance...isolate individual (clonal) colonies of bacteria carrying a specific plasmid. However, a liquid culture ...Concentration Ampicillin 100 µg/mL Bleocin 5 µg/mL Carbenicillin 100 µg/mL Chloramphenicol 25 µg/mL Coumermycin...
  3. Handling Plasmids from Addgene - Purifying Plasmid DNA

    Type
    Protocol
    ... g for 30 sec. Pour off the supernatant, being careful not to disturb the bacterial pellet. Resuspend ...Add 200 μL of Solution II and invert the tube carefully 5 times to mix the contents. The contents will...the supernatant into a new tube by pipetting or carefully pouring. Optional: Add 5 μL of 2 mg/mL RNase A...Pour out the supernatant in the sink. Pro-Tip Be careful, the pellet is harder to see and less well attached...
Showing: 581 - 600 of 643 results