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We narrowed to 951 results for: cha

Showing: 581 - 600 of 951 results

  1. Transferable Skills Guide: Public Speaking

    Type
    Blog Post
    ...Technical difficulties turn your slide deck into a chalk talk. Your translator doesn’t know the term “diving... speaking is hard. Aside from the diving board charades, I doubt anyone but me remembers any of the incidents...opportunities, and gives your entire audience a chance to comfortably engage with you. If you can give...

  2. Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics

    Type
    Blog Post
    ... Tsang AAVs are a great gene therapy delivery mechanism, but their small packaging capacity of 4-5 kb ...are proteins that detect and output the voltage changes in neural activity when paired with appropriate...green-fluorescent GEVIs and blue-light-sensitive channelrhodopsins. Find the Ace-mScarlet voltage indicator plasmids...

  3. Rosella: A Fluorescent pH-Biosensor for Studying Autophagy

    Type
    Blog Post
    ...abilities is that its fluorescence emission spectra changes when it goes from a more neutral pH compartment...conditions. Rosella makes it easier to study the mechanisms behind autophagy by tracking what’s being transported...17224625. 5. Noda, T., & Klionsky, D. J. (2008). Chapter 3 The Quantitative Pho8Δ60 Assay of Nonspecific...

  4. Seven Tips for Using LinkedIn as a Scientist

    Type
    Blog Post
    ...game for connecting to others.  Pro tip: You can change the option for sending LinkedIn messages to Click...without accidentally sending before you’ve had a chance to proof read. This might not be important for ...Turn off notifications to your network of profile changes. If you are working, and all of a sudden you start...

  5. Developing Lab Management Software for Biology

    Type
    Blog Post
    ...is placed on the Addgene website, it sets off a chain reaction of events throughout all of Addgene - within...If something happens to the worm that requires a change of tube, there’s no clean way to separate the contents...a separate worm model and tube model, we can interchange them easily as needed. In this case, the physical...

  6. Plasmids 101: The Wide World of Natural Plasmids

    Type
    Blog Post
    ...think of bacteria when we think of plasmids, both Archaea and Eukaryota can also harbor these circular DNA..., Moody, T., Rice, P. A., Yu, M. K., Mimee, M., Chang, E. B., Ruscheweyh, H.-J., Sunagawa, S., Mclellan...Plasmids Isolated from Lactic Acid Bacteria:Their Characteristics and Potential Applications in Biotechnology...

  7. Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene

    Type
    Blog Post
    ...NGS in 2017 for all incoming plasmids was a big change for Addgene - we had used Sanger sequencing for.... Priority number one was to ensure that this changeover had no impact on our ability to provide requesting...DNA Extracts. Pooled libraries are a bit more challenging to QC by NGS as they are often quite different...

  8. CRISPR 101: Anti-CRISPR Proteins Switch Off CRISPR-Cas Systems

    Type
    Blog Post
    ...longer Cas remains active in a cell, the greater chances there are for off-target edits to occur. Although...effects Prolonged Cas activity can increase the chance of off-target editing particularly after the majority...that have the gene deletion could replicate when challenged with the S. islandicus CRISPR-Cas system. 5. ...

  10. Early Career Researcher Toolbox: Finding Relevant Papers

    Type
    Blog Post
    ...particularly hard. There are millions of them. The real challenge is finding relevant papers. The latest installment...publications. Bonus: papers have to be summarized in 280 characters! Using Twitter to find papers  You’ll need to...a literature discovery platform developed by the Chan Zuckerberg Initiative. Meta is not a traditional...

  11. 6 Tips for Grant Writing

    Type
    Blog Post
    ...by the general public (either through taxes or charitable donations) so there is an increasing realization...funding or not; applications for funding from charities, commercial organizations, or universities may...-doc at Cambridge University where he studied mechanisms of cell division. Recently Seán got the entrepreneurial...

  12. Popular Retroviral Vectors and Their Uses in Scientific Research

    Type
    Blog Post
    ...gamma-retroviral packaging plasmids are not interchangeable. General envelope plasmids, such as VSV-G, ...interference (RNAi) is an RNA-mediated gene silencing mechanism. Scientists can express siRNAs to direct the cellular...that can reveal how genetic alterations lead to changes in phenotype. The plasmid comes with a 1.9 kb stuffer...

  13. Nanoblades: Tiny CRISPR Ninjas for Genome Editing Difficult Cells

    Type
    Blog Post
    ...a DNA sequence ranging from a single nucleotide change to large insertions like the addition of a fluorophore...retroviral transduction efficiency by neutralizing the charge repulsion between virions and the cell surface,...pronucleus of the zygote which is both technically challenging and can negatively impact zygote viability. Nanoblades...

  14. Plasmids 101: FLEx Vectors

    Type
    Blog Post
    ...will be greatly reduced, allowing for clearer characterization of the location, morphology, and circuit mapping...Schnütgen F, Doerflinger N, Calléja C, Wendling O, Chambon P, and Ghyselinck NB. A directional strategy for...and Sternson SM. 2008. A FLEX switch targets Channelrhodopsin-2 to multiple cell types for imaging and long-range...

  15. Troubleshooting and Optimizing a Western Blot

    Type
    Blog Post
    ... protein aggregation, you may want to consider changing your incubation temperature. Some proteins aggregate...kDa) and a 0.45 µm (p) membrane for larger ones. Changing your membrane size is probably the easiest troubleshooting...alcohol. The increased SDS will increase the negative charge on the protein, allowing it to move more readily...Posttranslational modifications Check literature Highly charged amino acids Run control protein (if available...

  16. Of Myc and Men

    Type
    Blog Post
    ...protein tagging. In two studies investigating mechanisms of endoplasmic reticulum localization, Munro ...also find anti-Myc [9E10] Fab fragments and single-chain variable fragments.  Abi, on the other hand...glucose-regulated protein and immunoglobulin heavy chain binding protein. Cell 46:291–300. https://doi.org...

  17. Mobile-CRISPRi: Bringing CRISPRi to Diverse Bacteria

    Type
    Blog Post
    ... to lab-adapted bacteria because it has been challenging to introduce CRISPRi machinery into diverse bacteria...antibiotics are often essential genes which creates a challenge when studying them since their complete knockdown...been a target for trimethoprim, there would be no changes in sensitivity. Pooled knockdowns are also possible...

  18. xCas9: Engineering a CRISPR Variant with PAM Flexibility

    Type
    Blog Post
    ...xCas9 3.6 displayed the second best PAM range. Characterizing xCas9 in mammalian cells Hu et al. compared...SpCas9. Hu et al. performed GUIDE-seq analysis to characterize genome-wide off-targets, since increasing PAM... with NGS) are shown as percentages, with fold changes compared to SpCas9 in parentheses. The development...

  19. Transferable Skills Guide: Managing a Team

    Type
    Blog Post
    ...individuals in your team you will have a much better chance of creating a high-performing team - which is every.... Personally I found this to be particularly challenging. As PhD students and postdocs we are used to ...the feedback, but at least you can start making changes for the best. Positive feedback is equally as important...

  20. 3D Printing Meets CRISPR Cas9

    Type
    Blog Post
    ...Byron Rubin, a crystallographer working with Jane Richardson, provided an initial solution to this problem...problem with the development of Byron’s Bender – a mechanical device that could be used to bend 1/8th inch ... the nascent polypeptide emerges from the exit channel.   He then said “I hadn’t realized how perfectly...
Showing: 581 - 600 of 951 results