We narrowed to 911 results for: TRO

...CRISPR-Csm: A novel RNA targeting system by: Susanna Stroik The Doudna lab’s recent work characterized a new...heat-inducible lentiviral plasmids here!   Wu Y, et. al. Control of the activity of CAR-T cells within tumours via...chemically stable tool for microscopy by: Susanna Stroik Microscopy techniques have been moving at the speed...microscopy applications such as super resolution, electron, and expansion microscopy. hfYFP is not only brilliantly...denaturing conditions, high temperatures, and other chaotropic conditions better than most FPs. The group also...expansion microscopy as well as correlative light and electron microscopy and observed high fluorescence resilience...

...plasmid deposit please sign up here.        A retroviral mouse CRISPR knockout library Article contributed...phenotype, and this depends on the method used to introduce the gRNAs into cells. Most CRISPR pooled libraries...issue, Sarah Teichmann and colleagues created a retroviral CRISPR knockout library using gRNAs designed ...designed by the Kosuke Yusa Lab. This retroviral library was used to transduce T helper type 2 (Th2) cells from...differentiation factors as the screen readout. This retroviral mouse CRISPR pooled library can be used for screening...concentration of ApoB-LPs has been shown to have a strong correlation with cardiovascular disease and indirect...

... to neuroscience. In fact, precise light-based control of biological processes is highly useful in other... to be optimized by Tabor’s lab, making light-controlled gene expression in E. coli easier and more robust...engineered to deliver precise, consistent, and controllable pulses of light. Complicated patterns of light...new pathways with sophisticated gene expression controls. Most cell types do not respond to low-intensity...two systems can be coexpressed for sophisticated control of gene expression. An E. coli light-sensitive...from this publication at Addgene. Multichromatic control of gene expression in Escherichia coli. Tabor JJ...

...sequence NGG TTTV PAM site preserved? Usually Destroyed Yes, Cpf1 cleaves 5' of the protospacer Multiplexing...Cassettes transfection gRNAs expressed from a polycistronic transcript  Requires co-transfection of Cas9...Cassettes transfection gRNAs expressed from a polycistronic transcript Requires co-transfection of Cas9  ...showing that Cpf1 can cleave an array of 4 crRNAs in vitro and when expressed in 293 cells. This allows for...a GFP tag. Four weeks post-infection, there was strong expression of Cpf1 and GFP in the targeted brain...effective method with multiple applications (in vitro, in vivo, transfection, viral transduction) for ...

...need to ensure you have a bead-only control alongside your input control. Elute the DNA after washing and..., which uses formaldehyde or UV light to create strong but reversible bonds. This will keep the protein...of histone modifying proteins, which bind quite strongly to DNA, crosslinking may not be necessary. This...Enzymatic digestion, while gentler on the sample, introduces bias due to the selective nature of the cutting...Antibodies 101: Polyclonal Antibodies Antibodies 101: Introduction to Antibodies Resources on Addgene.org ...

...regeneration studies, and cancer biology. I was first introduced to bioelectricity when I attended the Developmental...living cells. Since bioelectrical signals are controlled by the opening and closing of ion channel proteins...genetically encoded, light-gated ion translocators to control tumorigenesis." Oncotarget 7.15 (2016): 19575. ...signaling networks: exploiting voltage gradients for control of growth and form." Annual review of biomedical... neural and gap junction protein-mediated cues control polarity during planarian regeneration." Developmental...Vaibhav P., et al. "Transmembrane voltage potential controls embryonic eye patterning in Xenopus laevis." Development...

...Be prepared to troubleshoot and be sure to include proper positive and negative controls as you get your...someone to turn to for advice if you need to troubleshoot. What do you know about the vendor and the vendor...anti-tag antibody; and immunoprecipitation-mass spectrometry strategies that isolate an antibody’s immune...complexes and confirm interacting partners by mass spectrometry (Uhlen, 2016). Be sure that the antibody you...The Basics of Western blottingAntibodies 101: Introduction to AntibodiesAntibodies 101: Epitope availability...

...Scientific Support Team here at Addgene, so troubleshooting molecular biology experiments was in the job...core part of our academic training.  Developing strong problem solving skills is just a matter of applying...think back to the last experiment you had to troubleshoot; you probably did not just automatically come...As such, scientists are uniquely prepared to be strong problem solvers both in and out of the lab. The...position. Of course, there are limits to how far strong problem solving skills alone can take you - just...effectively does not mean I am qualified to be an astronaut. But, knowing that this skillset is a part of ...

...research that was done and what it means. The introduction should give you just enough information to allow... addressed. In primary research papers, the introductions should be fairly focused. Consult a review article... etc)? Did they perform all of the appropriate controls? Refer back to the methods section for finer points...automate the process as well. Sign up for eTOC (electronic table of contents) alerts from journals you read...useful papers. For many, this collection is purely electronic. Gone are the days of file cabinets filled to...She is a fan of photosynthesis and writes about autotrophs and biochemistry on her blog. Follow her on Twitter...

...activity in HEK293 by reducing editing from ~30% in control samples to 0-10% in Acr samples. Acr proteins can...When added to human cells six hours after the introduction of Cas9 RNPs, AcrIIA4 reduced off-target editing...specific site. 2. Temporal, spatial, or conditional control of CRISPR activity Regulating Acr proteins by inducible...molecules could achieve rapid, dynamic, and spatial control of CRISPR-Cas activity. For example, fusing AcrA4II...LOV2 domain allows anti-CRISPR activity to be controlled by blue light (Bubeck et al., 2018). 3. Reduce...Your Next Experiment Read more about Optogenetic Control of CRISPR   Resources on Addgene.org Find CRISPR...