We narrowed to 929 results for: INA

1. Antibodies 101: Stripping and Reprobing Western Blots

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   Blog Post

   ...strength of bands from different rounds of detection. Finally, stripping and reprobing works best with western...

2. Progress Towards a PAM-Free CRISPR

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   ...are not in your favor. Thus, there is a need to eliminate or reduce the PAM requirement to allow flexibility...

3. Open Resources and Plasmid Tools For Studying C. elegans

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   ...microscopy and computer imaging processing. The combination of these methods provides unique opportunities...

4. Viral Vectors 101: Calcium Sensors

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   ...state only by simultaneous (1) user-controlled illumination with violet light and (2) exposure to increased...

5. Antibodies 101: Choosing the Right Isotype

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   ...the background staining but they do not always eliminate the issue (Lu 1998). Consequently, if you find...

6. Early Career Researcher Toolbox: Finding Relevant Papers

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   ...authors by exploring author pages (for example, Melina Fan’s author page). These pages show all publications...

7. A Guide to Designing a Scientific Poster: Content, Layout, and More

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   ...other ways to make your poster more accessible. Final thoughts Though we’ve just presented some standard...

8. Nanoblades: Tiny CRISPR Ninjas for Genome Editing Difficult Cells

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   ...proof-of-principle, the team used Nanoblades to disrupt the tyrosinase (Tyr) gene in mouse zygotes, which results in...

9. Which Fluorescent Protein Should I Use?

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   ...GFP also emits yellow light). Note that some combinations of FPs can cause an effect called FRET (fluorescence...

10. CRISPR 101: Mammalian Expression Systems and Delivery Methods

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    ...transduction or injection as either protein, RNA, or DNA. Finally, once you have identified the best expression system...

11. Magnetic Control of Proteins: More than a Dream

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    ...possibility that this might just be an artifact and eliminate sources of interference, but the result persisted...

12. Antibodies 101: Flow Compensation

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    ... you should be able to run your experiment and finalize your compensation values later. This can be helpful...

13. Transferable Skills: Negotiation

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    ...Creative negotiation can also decrease or entirely eliminate the feeling of conflict many of us associate with...

14. Antibodies 101: Conventional vs Spectral Flow Cytometry

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    ...Antibody GuideAddgene's collection of ready-to-use recombinant antibodies...

15. xCas9: Engineering a CRISPR Variant with PAM Flexibility

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    ...of only the broadest-targeting variants. For the final round of evolution, they used a no-G PAM library...

16. When is a Monomer not a Monomer? The Top Three Ways Your Favorite Fluorescent Protein Oligomerizes in Cells

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    ...contain two dimerizing FPs can exist in an open inactive state, a closed active state, and an artificially...

17. Interview: Hodaka Fujii on enChIP, New CRISPR Tools, and More

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    ...colleagues at NYU and visiting scientists, such as seminar speakers. They made an every effort to interact...

18. A Guide to Starting Your Own Journal Club

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    ...polls and surveys. A mix of closed (multiple choice/binary) and open questions is helpful to ensure acquisition...

19. Neuronal labeling with Spaghetti Monster

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    ...neuronal networks. For almost three decades, recombinant Adeno-associated virus (AAV) has been widely ...

20. Plasmids 101: Shuttle Vectors

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    ...for simple transfection experiments. Reporters Finally, in some cases a researcher may choose not impose...

21. Designing Your Chalk Talk for the Academic Job Interview

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    ...new directions, while the second approach could terminate with the rejection of a model and no clear alternatives...

22. Plasmids 101: Plasmid Incompatibility

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    ...Plasmid incompatibility is defined as the inability of different plasmids to be maintained in one bacterial...

23. An Inside Look into Addgene's Dev Team

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    ...likely different from what I would have done. And finally the stakeholder accepts the new feature as done...

24. Transferable Skills Guide: Career Planning Resources

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    ...you don’t get the specific position he or she originally called about, you can keep in touch and reach...

25. 3 Tips to Improve HDR Efficiency for CRISPR Editing in Human Cells

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    ... cells, HDR is relatively rare and DSBs are predominantly repaired by NHEJ. While other groups have focused...

26. Antibodies 101: Normalization and Loading Controls for Western Blots

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    ...or membrane (depending on your approach) as your final blot. Quantifying the entire lane of protein provides...

27. Plasmids 101: Biotinylation

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    ...PubMed PMID: 20353954. 5. Kay, B., Thai, S., & Volgina, V. “High-Throughput Biotinylation of Proteins....

28. Quick Guide to Near-Infrared Fluorescent Proteins

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    Blog Post

    ...normalization to fluorescence of co-transfected EGFP. e Originally reported as a monomer16, IFP2.0 was later found...

29. Is this the right place for me? 8 tactics for choosing a lab

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    Blog Post

    ...t suit my collaborative learning style at all. Finally I rotated in a small lab with a new PI. I realized...