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Plasmids 101: Golden Gate Cloning

Type

Blog Post

... The destination vector and entry vector(s) are placed in a single tube containing the Type IIS enzyme...
Writing Scientific Manuscripts: Literature Searching, Reading, & Organizing

Type

Blog Post

... – F1000 just announced the launch of their F1000Workspace. Give your favorite searching, reading and...
Career Coaching for Scientists: Why and Where Do I Find One?

Type

Blog Post

...articles. Look for them on Twitter, LinkedIn and Facebook to see their online personas. Some have online...
5 Steps to Writing Grant Proposals for Grad Students

Type

Blog Post

...research plan Most grant applications restrict the space available to describe the proposed work. Thus, every...
Adapting Toehold Switches to Detect Zika Virus

Type

Blog Post

...this spread until vaccine programs can be put in place to provide community protection. Toehold switches...
Plasmids 101: FLEx Vectors

Type

Blog Post

...gene “GeneR”). The key to this experiment is the placement of the two pairs of target sites. By flanking ...
Tips for Titering Your Lentiviral Preps

Type

Blog Post

...or single cell sorting based on expression of a surface marker, subcloning, expansion, and confirmation...
Adenoviral Vector Production and Troubleshooting

Type

Blog Post

...chloride (CsCl) density gradients combined with ultracentrifugation to separate rAdV from other cellular debris...
Science communication: 9 Strategies to Get Your Foot in the Door

Type

Blog Post

...opportunity is the AAAS Mass Media Fellowship, which places science students for 10 busy weeks at media outlets...
Fluorescent Proteins 101: Introduction to FRET

Type

Blog Post

...Miyawaki et al., 1997), was designed to measure intracellular calcium and published in 1997. Since that time...
Corralling and Cornering the Goldilocks Antibody

Type

Blog Post

... contained all the goldilocks antibodies in one place... well, lucky for you one is in the making by Dr...
Split Fluorescent Proteins for Studying Protein-Protein Interactions

Type

Blog Post

...fragment An enhanced recombinant amino-terminal acetylation system and novel in vivo high-throughput screen...
Simple CRISPR-based Epigenetic Editing: dCas9-directed DNA Demethylation

Type

Blog Post

... godsends. Tethering dCas9 to the histone acetyltransferase p300 or the DNA methyltransferase DNMT3A allows...
CRISPR 101: Anti-CRISPR Proteins Switch Off CRISPR-Cas Systems

Type

Blog Post

... viruses. For example, an Acr gene was used to replace genes in the difficult-to-engineer Sulfolobus islandicus...
CRISPR in the Clinic

Type

Blog Post

...for these diseases was sponsored by Vertex Pharmaceuticals and CRISPR Therapeutics. Data shared from these...
Open Resources and Plasmid Tools For Studying C. elegans

Type

Blog Post

...reminded of the extent that C. elegans researchers embrace open science and share resources and tools. That...
Mouse Modeling, Part 1: Genetically Engineered Mice

Type

Blog Post

...create knockout mice include a reporter gene, which replaces the targeted gene and provides a way to track ...
CRISPR Activators: A Comparison Between dCas9-VP64, SAM, SunTag, VPR, and More!

Type

Blog Post

...method, dCas9 is fused to CBP, an histone acetyltransferase domain capable of rearranging chromatin structure...
7 Simple Strategies to Resolve Conflicts with Difficult Supervisors

Type

Blog Post

...both personal and professional conflicts. At the workplace, these strategies will help you to communicate...
Google Forums Round Up: First Impressions of NgAgo

Type

Blog Post

...community if you've had success with it. Some great places to get your results out there include: Google ...

Showing: 601 - 620 of 720 results

Results per page:

Plasmids 101: Golden Gate Cloning

Writing Scientific Manuscripts: Literature Searching, Reading, & Organizing

Career Coaching for Scientists: Why and Where Do I Find One?

5 Steps to Writing Grant Proposals for Grad Students

Adapting Toehold Switches to Detect Zika Virus

Plasmids 101: FLEx Vectors

Tips for Titering Your Lentiviral Preps

Adenoviral Vector Production and Troubleshooting

Science communication: 9 Strategies to Get Your Foot in the Door

Fluorescent Proteins 101: Introduction to FRET

Corralling and Cornering the Goldilocks Antibody

Split Fluorescent Proteins for Studying Protein-Protein Interactions

Simple CRISPR-based Epigenetic Editing: dCas9-directed DNA Demethylation

CRISPR 101: Anti-CRISPR Proteins Switch Off CRISPR-Cas Systems

CRISPR in the Clinic

Open Resources and Plasmid Tools For Studying C. elegans

Mouse Modeling, Part 1: Genetically Engineered Mice

CRISPR Activators: A Comparison Between dCas9-VP64, SAM, SunTag, VPR, and More!

7 Simple Strategies to Resolve Conflicts with Difficult Supervisors

Google Forums Round Up: First Impressions of NgAgo

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