CRISPR Library Amplification
Type
Protocol
...sufficient quantities of library for experimental applications. Repeated amplifications should be avoided as... of a library of 100,000 plasmids. Colonies may appear small and require extra incubation time in order... as the quantification method. If all Maxipreps appear to contain sufficient DNA for use, pool samples...purifications. Consult the manufacturers handbooks for appropriate volumes and numbers of tandem purifications. ...