... find plasmids badge appear So how do you make this magical Addgene badge appear? First you’ll need to...

... Dives” series comes in! We’ll take a common application or concept and go beyond the basics - diving ...you’re ready for the next steps on some common applications, join us as we get into the weeds on some of...

...an organism’s genome usually for a research or applied goal. Components of CRISPR: gRNAs: Sequences ...produce either a gRNA, a nuclease, or both. Applications of CRISPR: Cut: CRISPR can be used to cut the...

...with your manager early on. Chapter 4: Crystal's approach to management and the importance of realizing ... also adapting to needs as they arise. Tips on applying for industry jobs and deciphering job listings...

...awarded plasmids from Addgene as a successful applicant to the Addgene, Seeding Labs Plasmid Grant. For...scientist's molecular biology needs and why Kwabena applied for the Addgene, Seeding Labs plasmid grant. 7:...

... in 100 µL aliquots, so the volume options will appear as 10, 25, or 50 units.  Once your request is received... you find this service useful in your research. Happy vectoring! ...

... certain mystique that makes them difficult to approach for the youngster just starting in the lab, but... a graduate student? 4:50 - 8:00 : How did you approach doing rotations? 8:01 - 11:25 : How did you decide...

...that it is also possible that their system may be applied to efficient viral vector-mediated multiplex genome...genes at the same time.” For more details on how to apply the new system, see Yamamoto and Sakuma’s protocol...

...night, so why leave your lab equipment on? This applies to water baths, computers, and more. Consolidate...to the Addgene blog Learn about other creative approaches for lab sustainability Resources on Addgene....

...when budgets are constrained. This collaborative approach eliminates redundant work, saving time and allowing...be listed as direct costs from a CRO in grant applications, addressing the new funding structures and indirect...

... shipped! That's a 1/2 million plasmids sent to happy scientists around the world in ~10 years! We're ... as of March 26, 2015. How does Addgene make it happen? Our awesome team of Addgenies! Our team recently...

...talks, symposiums, poster sessions, exhibit booths, happy hour socials, and late, late night socials. As a...depositing scientists at #SfN14. And we've been so happy to hear that we've been able to help their labs ...

...pool has been sufficiently selected with the appropriate antibiotic so that every cell in the culture ...stable cells such that the next day they will be approximately 50–60% confluent. For 293T cells this is about...confluent or the medium is acidified (i.e., the medium appears orange/yellow if using a standard phenol red pH...counting the cells. When you ultimately transfer approximately 50–100 cells to the conditioned medium, it will...solution at a concentration of 5 cells/mL. Prepare approximately 10 mL of this cell solution for each 96-well...which you see growth. At this time, the cells will appear as colonies in the well ( Figure 1 ). You will ...

...: The Education & Tools menu Further down What happens if you do scroll down? You’ll find Blugene, as ...through the yellow popup at the bottom of the page. Happy surfing!   This post was written by Addgenies Jo...

...effective practices and disseminating career training approaches in a way that can be implemented widely in the...training programs. I believe in the academic apprenticeship system as a training ground for scientists,...

...where I foster dogs and help screen dog adoption applications. My most recent foster dog was with us for two...cancer through the sale of art. Past volunteer appointments included the Belmont Human Rights Commission...

... to curate the perfect library, obtained the appropriate cDNAs from other researchers, and cloned them... make the library usable for pooled screens. Applications of the cancer pathways ORFs kit Wood and his...

...contains the expected size insert. By selecting the appropriate enzyme(s), one can either linearize a plasmid...interest (see below for recommended amounts) Appropriate restriction enzyme (see manufacturer's instructions...instructions for proper amount) Approrpriate restriction digest buffer (see manufacturer's instructions) Gel...others. When choosing restriction enzymes for this approach, it is often a good idea to choose two different...products on a gel and those clones with the appropriately sized products should have the correct orientation...

...container Personal protective equipment (PPE) appropriate for the biosafety level of the lab space Reagents... like the one in the picture above. Wear PPE appropriate for the lab space in which you are working. Even...that the centrifuge is clean and that everything appears to be working smoothly. Place your sample tubes... the centrifuge so that the pellets form in approximately the same place in each sample. Place the centrifuge...according to speed and time (and temperature, if applicable) listed in your protocol. Pro-Tip Spin speed ...

... swing dance event,” Zheng says (networking can happen anywhere!). The pair quickly teamed up to create...researcher may only know how to prepare phage for one application but not another or they may have only used phage...