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Addgene

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Showing: 601 - 620 of 954 results

  3. Addgene Stands with the Scientific Community

    Type
    Blog Post
    ...when budgets are constrained. This collaborative approach eliminates redundant work, saving time and allowing...be listed as direct costs from a CRO in grant applications, addressing the new funding structures and indirect...

  4. 500,000 Plasmids Shipped and Counting!

    Type
    Blog Post
    ... shipped! That's a 1/2 million plasmids sent to happy scientists around the world in ~10 years! We're ... as of March 26, 2015. How does Addgene make it happen? Our awesome team of Addgenies! Our team recently...

  5. Fueled by Coffee at #SfN14

    Type
    Blog Post
    ...talks, symposiums, poster sessions, exhibit booths, happy hour socials, and late, late night socials. As a...depositing scientists at #SfN14. And we've been so happy to hear that we've been able to help their labs ...

  6. Addgene's Website Update

    Type
    Blog Post
    ...: The Education & Tools menu Further down What happens if you do scroll down? You’ll find Blugene, as ...through the yellow popup at the bottom of the page. Happy surfing!   This post was written by Addgenies Jo...

  9. Protocol - How to Run an Agarose Gel

    Type
    Protocol
    ...than longer ones. Thus, you can determine the approximate length of a DNA fragment by running it on an ... bromide (EtBr) to a final concentration of approximately 0.2-0.5 μg/mL (usually about 2-3 μl of lab stock... bands will be differentially intense. If this happens, you can just soak the gel in EtBr solution and...Run the gel at 80-150 V until the dye line is approximately 75-80% of the way down the gel. A typical run...are usually referred to as ‘bands’ due to their appearance on the gel. Pro-Tip If you will be purifying ...

  13. Coomassie Purity Stain of Recombinant Antibodies

    Type
    Protocol
    ...microcentrifuge. Remove the gel from the plastic wrapper and rinse with deionized water. Gently remove the...5 µL of the prestained protein ladder to the appropriate well. Pro-Tip When possible, skip one lane before...µL of each recombinant antibody sample to the appropriate well. Pro-Tip Leaving clear lanes between samples... Take a brightfield image of the gel with an appropriate imaging system. Figure 1 Recombinant antibody...Gels . Select Plot Lanes . One graph per lane will appear with peaks representing each protein band. Use ...

  14. New Norepinephrine Indicators: nLightG and nLightR

    Type
    Blog Post
    ...neurons expressing nLightG or nLightR before/after application of NE (10 μM) and corresponding pixel-wise ΔF...GFP), and nLightR uses a red fluorescent protein, mApple. Kagiampaki et al. found nLight is suitable for...

  16. 9 Tips to Achieve Success in Academia

    Type
    Blog Post
    ...emphasizes that people should “follow their passions, be happy, and match their personal goals to their career ...analytical and critical thinking skills that are applicable to a variety of careers outside of academia. ...

  17. Bringing Sustainable Practices to the Lab: Recycling

    Type
    Blog Post
    ... at least we can reduce the rate at which that happens and reduce the manufacturing of new containers,...bin or box in your tissue culture room for the wrappers and ship them back. Start a glove recycling ...

  18. Fostering communication in remote collaborations

    Type
    Blog Post
    ...willingness of the team to adopt various tools, this may happen over email or using rapid communication tools such...access data and drafts of manuscripts and grant applications in real-time so they can provide input at the...

  20. CASTing Off for New Shores in Human Genome Editing

    Type
    Blog Post
    ...individualized optimizations, safety testing, and approval. In contrast, a system that can deliver a healthy...could provide a streamlined, one-size-fits-most approach. evoCAST is an important step toward that kind...
Showing: 601 - 620 of 954 results