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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 644 results for: CAR

Showing: 601 - 620 of 644 results

  2. Antibody Validation Using the Indirect ELISA Method

    Type
    Protocol
    ...Cap the bottle and invert several times to mix. Carefully remove the plate seal from the 96-well plate and...4 °C . Section 3: Primary antibody incubation Carefully remove the plate seal from the 96-well plate and...°C . Section 4: Secondary antibody incubation Carefully remove the plate seal from the 96-well plate and...or overnight at 4 °C . Section 5: TMB reaction Carefully remove the plate seal from the 96-well plate and...

  5. Plasmids 101: Restriction Cloning

    Type
    Blog Post
    ...restriction digest and ligation process, you should carefully choose your backbone and insert - these both must...

  7. Antibodies 101: Validation

    Type
    Blog Post
    ...10.1016/j.nbt.2018.08.002  Uhlen M, Bandrowski A, Carr S, Edwards A, Ellenberg J, Lundberg E, Rimm DL, ...

  14. Lentivirus Production

    Type
    Protocol
    ...stored at 4 °C for up to 2 months. After 2 months, discard the tube and thaw a new working stock. The optimal...the transfection mix onto the 10 cm plate, being careful not to disturb the cells. Incubate the cells for...the following morning. The following morning, carefully aspirate the media. Replace the media with 10 ...

  16. AAV Titration by qPCR Using SYBR Green Technology

    Type
    Protocol
    ...polymerase and a blend of dTTP/dUTP to minimize carryover contamination. The master mix should also contain...DNase I to eliminate any contaminating plasmid DNA carried over from the production process (DNase does not... 3 Pro-Tip To help stabilize the standards add carrier DNA to a final concentration of 4 ug/mL to each...

  18. CRISPR Library Amplification

    Type
    Protocol
    ...agar. This usually takes 1-2 minutes. Critical Be careful not to rip or shred the agar. Do so by gentle spreading...Pushing motion is better than pulling motion Take care not to split or gouge agar during the scraping process...library even in the face of a recombined fraction but care must be taken to perform adequate NGS based QC to...

  20. Weighing Reagents Protocol

    Type
    Protocol
    ...spills produced during the protocol are cleaned up. Discard the weighing boat or weighing paper in the trash...weighed is not biohazardous. If it is biohazardous, discard the weighing boat or paper in the biohazard solid...
Showing: 601 - 620 of 644 results