We narrowed to 644 results for: CAR

... g for 30 sec. Pour off the supernatant, being careful not to disturb the bacterial pellet. Resuspend ...Add 200 μL of Solution II and invert the tube carefully 5 times to mix the contents. The contents will...the supernatant into a new tube by pipetting or carefully pouring. Optional: Add 5 μL of 2 mg/mL RNase A...Pour out the supernatant in the sink. Pro-Tip Be careful, the pellet is harder to see and less well attached...

...Cap the bottle and invert several times to mix. Carefully remove the plate seal from the 96-well plate and...4 °C . Section 3: Primary antibody incubation Carefully remove the plate seal from the 96-well plate and...°C . Section 4: Secondary antibody incubation Carefully remove the plate seal from the 96-well plate and...or overnight at 4 °C . Section 5: TMB reaction Carefully remove the plate seal from the 96-well plate and...

... the western blot. When planning a western, take care to include the proper positive and negative controls...

...restriction digest and ligation process, you should carefully choose your backbone and insert - these both must...

...References and Resources References Chan, A. C., & Carter, P. J. (2010). Therapeutic antibodies for autoimmunity...

...protein level. The “correct” choice of tool requires careful balancing of the inherent advantages and limitations...

...10.1016/j.nbt.2018.08.002  Uhlen M, Bandrowski A, Carr S, Edwards A, Ellenberg J, Lundberg E, Rimm DL, ...

...system is to use a lambda red phage, λTetR, that carries the tetracycline resistance gene and the lambda...

...see the movies demonstrating Ca2+ dynamics in cardiomyocytes. Enhanced Nano-Lantern constructs are available...

...fluorophores and their emission spectra. Some fluorophores carry their emission peaks within their names, for example...

... PMCID: PMC5312033. 2. Dewari PS, Southgate B, Mccarten K, Monogarov G, O'Duibhir E, Quinn N, Tyrer A,...

...: Immunofluorescence staining for CD31 (green), CARD14 (red), and pNFkB (blue) in lesional skin from a...

...strategies are presented (Fig. 1) which can be carried out using Addgene plasmids. His-tag labeling reagents...

...plasmids from this paper at Addgene. 12. Ronda, Carlotta, et al. (2016). "CRMAGE: CRISPR Optimized MAGE...

...stored at 4 °C for up to 2 months. After 2 months, discard the tube and thaw a new working stock. The optimal...the transfection mix onto the 10 cm plate, being careful not to disturb the cells. Incubate the cells for...the following morning. The following morning, carefully aspirate the media. Replace the media with 10 ...

...polymerase and a blend of dTTP/dUTP to minimize carryover contamination. The master mix should also contain...DNase I to eliminate any contaminating plasmid DNA carried over from the production process (DNase does not... 3 Pro-Tip To help stabilize the standards add carrier DNA to a final concentration of 4 ug/mL to each...

...editing is precise and versatile, but it requires careful optimization to achieve the best efficiency. The...

...This blog post was originally written by Caroline LaManna, published Mar 8, 2016. The updated and expanded...

...agar. This usually takes 1-2 minutes. Critical Be careful not to rip or shred the agar. Do so by gentle spreading...Pushing motion is better than pulling motion Take care not to split or gouge agar during the scraping process...library even in the face of a recombined fraction but care must be taken to perform adequate NGS based QC to...

...spills produced during the protocol are cleaned up. Discard the weighing boat or weighing paper in the trash...weighed is not biohazardous. If it is biohazardous, discard the weighing boat or paper in the biohazard solid...