We narrowed to 757 results for: Dos

Comparing and contrasting Cas9 to the other genome editing-capable Cas family proteins.

A growing area of scientific communication is preprints, a strong open science resource explored in this post.

Polyclonal antibodies are a common research reagent - learn how they’re produced and the advantages and disadvantages of using them in your next experiment.

GPCRs are receptors that respond to stimuli and elicit cellular responses. Learn about the parts of GPCRs, how they work, and tools to study them.

Wondering what a software product manger does? Check out this article to see a day in the life of a software product manager in the life sciences.

New channelrhodropsins, called ChRgers, avoid intracranial injections and fiber-optic cable implantation for in vivo optogenetics experiments.

All living cells possess a bioelectric state. Learn about how bioelectricity can advance research in birth defects, regeneration, medicine, and synthetic bioengineering

Getting start with mouse models? Here, we break down how to cross mice to create double knockout lines and Cre-lox lines.

Gene expression is often imprecise due to variations in gene copy number within the cell (either on plasmid or genome). New promoters from Chris Voigt’s lab enable stable gene expression regardless of copy number regardless of growth phase and whether they are expressed on the plasmid or chromosome.

Learn how you can use RaPID to detect RNA-protein interactions and its applications in infectious and human genetic diseases.

Learn how you can visualize newly synthesized proteins with FUNCAT-PLA.

A paper published in Nature used CRISPR to correct a disease-causing mutation in human embryos. Does this paper herald a future of human germline editing?

With it's small crRNAs and RNase activity, Cpf1 can easily be used for multiplex genome editing. Learn more about new multiplex Cpf1 tools here.

FLEx vectors utilize Cre/Lox to conditionally manipulate gene expression in vivo.

Rationally engineered Cas9 variants eSpCas9 SpCas9-HF1 enhance CRISPR on-target specificity. Learn how in this post.

Five steps to help graduate students and postdocs write their first grant proposal.

Learn about Addgene's QC process and how and why we maintain a high standard of sequence accuracy across our plasmid collection.

Learn about the different open resources for C. elegans like OpenWorm, microPublication, and 3'UTRome, and find C. elegans tools at Addgene.

What you get out of a conference depends on what you put into it before, during, and after the meeting. Read on to find tips for honing your elevator pitch, the elevator pitch, navigating talks, networking, and social media.

Find tips and best practices for producing lentivirus in your lab: from choosing a packaging system to optimizing transfection and improving titers.