...colony of genetically engineered mice, what can you do? In this post, we’ll cover why and how to cross mice...

... unconjugated version, but if you find yourself doing your own conjugation, it is important to keep in...

...H134R) both reach the brain, but individual neurons do not express as many copies of the ChRs as compared...

...RaPID can be used to study RNA-protein interactions. Do you have a question that RaPID could help answer?...

...diagnosis (PGD) screens embryos to find those that do not carry a given dominant disease-causing gene, ...

...Writing Scientific Manuscripts by Johnna Roose Dos & Don'ts When Publishing a Scientific Manuscript ...

...application process? What types of writing feedback do you find most helpful? Have a great grant writing...

...Addgene.org Find Info on How to Quantify Your DNA Learn to Do a Bacterial Transformation Learn How to Perform a...

...study of heritable changes in gene expression that do not involve any changes to DNA sequence (Epigenetics...

... provided you with a few ideas to consider if you do run into a roadblock and encourage you to check out...overcome this issue, we recommend using a range of doses of lentivirus when transducing a target cell line...transduction experiment. Media choice, reaction vessel, dose of virus, volume and timing of transduction will...

...Despite their advantages, mammalian expressions systems do require demanding cell culture conditions compared...

...after knockdown of beta-catenin. Other gene targets do not require amputation to be visible. For example...

...  Listen to the CRY2clust podcast segment The Won Do Heo lab at the Korea Advanced Institute of Science...

...been used to treat 10 infants with this disease. To do so, their T-cells were grown in vitro, their mutations...to be untreatable until 2008. Following a single dose of subretinal administration of rAAV2 carrying the...

...backbone. Purpose Answer “What does this plasmid do?” in <200 characters. A descriptive purpose focuses...

.... Fun fact! Some older plasmids in our collection do not have a full sequence, as they were deposited ...

...Doench, “Whole plasmid sequencing is just part of doing good science!” Find the Fragmid kit here!  Cloning...

...every C.elegans  neuron. Scientists are currently doing this using two approaches: single-cell RNA-sequencing...

...blurb you will use to introduce yourself and what you do. You might want to include your university and your...

...buffer as the one in the gel box (do not mix different buffers and do not use water). Microwave for 1-3...instructions on how to do this, visit the Gel Purification page. Tips and FAQ How do you get better resolution...min until the agarose is completely dissolved (but do not overboil the solution, as some of the buffer ...to the running buffer when you run the gel. If you do not add EtBr to the gel and running buffer, you will...the DNA in your sample lanes. For more details on doing diagnostic digests and how to interpret them please...typically used to separate 5 - 500 bp fragments. How do you get better separation of bands? If you have similarly...