We narrowed to 770 results for: Dos;
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TypeBlog Post...: A New Tool for CRISPR Genome Editing Which Cas9 Do I Choose for My CRISPR Experiment A Match Made in...
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CRISPR 101: Cas9 vs. The Other Cas(s)
TypeBlog Post...resources on the Addgene Blog CRISPR 101: Which Cas9 do I choose for my CRISPR experiment? The PAM requirement... -
Mouse Modeling, Part 2: Breeding and Crossing Mice
TypeBlog Post...colony of genetically engineered mice, what can you do? In this post, we’ll cover why and how to cross mice... -
Channelrhodopsins with improved light sensitivity for minimally-invasive optogenetics
TypeBlog Post...H134R) both reach the brain, but individual neurons do not express as many copies of the ChRs as compared... -
Plasmids 101: FLEx Vectors
TypeBlog Post...off"). These Cre-off switches may be referred to as DO (Double-floxed Orientation) and could be used to ... -
Enhancing CRISPR Targeting Specificity with eSpCas9, SpCas9-HF1, & HypaCas9
TypeBlog Post...strand breaks (BLESS), revealed that these mutants do not cause off-target effects at unanticipated sites... -
Writing Scientific Manuscripts: Literature Searching, Reading, & Organizing
TypeBlog Post...Writing Scientific Manuscripts by Johnna Roose Dos & Don'ts When Publishing a Scientific Manuscript ... -
5 Steps to Writing Grant Proposals for Grad Students
TypeBlog Post...application process? What types of writing feedback do you find most helpful? Have a great grant writing... -
A Look at Addgene's QC Process
TypeBlog Post.... Fun fact! Some older plasmids in our collection do not have a full sequence, as they were deposited ... -
Making CRISPR Plasmids Using Fragmid
TypeBlog Post...Doench, “Whole plasmid sequencing is just part of doing good science!” Find the Fragmid kit here! Cloning... -
Overcoming the Challenges of Lentiviral Production
TypeBlog Post... provided you with a few ideas to consider if you do run into a roadblock and encourage you to check out...overcome this issue, we recommend using a range of doses of lentivirus when transducing a target cell line...transduction experiment. Media choice, reaction vessel, dose of virus, volume and timing of transduction will... -
Plasmids 101: Protein Expression
TypeBlog Post...Despite their advantages, mammalian expressions systems do require demanding cell culture conditions compared... -
Educational Planarian Tools for Teaching Developmental Biology Techniques
TypeBlog Post...after knockdown of beta-catenin. Other gene targets do not require amputation to be visible. For example... -
Hot Plasmids May 2018 - Optogenetics, Decaffeination, Biosensors, and Fluorescent Protein Tools
TypeBlog Post... Listen to the CRY2clust podcast segment The Won Do Heo lab at the Korea Advanced Institute of Science... -
Adeno Associated Virus (AAV) for Cell and Gene Therapy
TypeBlog Post...been used to treat 10 infants with this disease. To do so, their T-cells were grown in vitro, their mutations...to be untreatable until 2008. Following a single dose of subretinal administration of rAAV2 carrying the... -
Quickest Way to Deposit Plasmids: The Deposit Spreadsheet
TypeBlog Post...backbone. Purpose Answer “What does this plasmid do?” in <200 characters. A descriptive purpose focuses... -
Open Resources and Plasmid Tools For Studying C. elegans
TypeBlog Post...every C.elegans neuron. Scientists are currently doing this using two approaches: single-cell RNA-sequencing... -
Tips to Make the Most of a Scientific Conference
TypeBlog Post...blurb you will use to introduce yourself and what you do. You might want to include your university and your... -
Plasmids 101: Optimizing Plasmid Yields
TypeBlog Post...Addgene.org Find Info on How to Quantify Your DNA Learn to Do a Bacterial Transformation Learn How to Perform a... -
Illuminating Epigenetics with A FRET Based Biosensor
TypeBlog Post...study of heritable changes in gene expression that do not involve any changes to DNA sequence (Epigenetics...