Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

We narrowed to 629 results for: EMB

Showing: 601 - 620 of 629 results

  5. Finding Your Science Policy Path

    Type
    Blog Post
    ...backgrounds. In fact, many of my AAAS STPF cohort members came to DC to do either a sabbatical year or transition...

  9. Typing CRISPR Systems

    Type
    Blog Post
    ...fact have a helicase domain due to a gene that resembles cas10. All type IV subtypes are missing adaptation...

  16. Using AAV for Neuronal Tracing

    Type
    Blog Post
    ... natural RABV or HSV can). AAV does not have a membrane envelope like lentivirus, but instead is contained...

  17. Modular Cloning Guide

    Type
    Guide
    ...modules. In the first assembly step, compatible Level 0 parts are directionally assembled into a Level 1 vector...MetClo DNA Assembly Kit Bacterial Expression Christopher A. O'Callaghan Toolkit for assembling large DNA...subunits are first assembled by Golden Gate cloning and then combined by Gibson Assembly into acceptor vectors...transfections. PUF Assembly Kit Mammalian Expression, Bacterial Expression Huimin Zhao Plasmids to assemble custom-made...pFUS_B4 clones for Golden Gate assembly of 15-RVD TALENs. FusX TALEN Assembly System Kit Genome Engineering...Ekker 336 plasmids of pre-assembled pFusX clones allowing for Golden Gate assembly of 15-16 RVD TALEN inserts...Cloning (or MoClo) is a system for efficiently assembling multiple DNA parts into functional plasmids. ...

  18. Molecular Cloning Techniques

    Type
    Guide
    ... Created with BioRender.com. Gibson Assembly (Isothermal Assembly Reaction) Isothermal cloning, more commonly...Read more in our Gibson Assembly blog post . Figure 4: Summary of Gibson assembly. Created with BioRender.com... commonly known as Gibson assembly, takes advantage of the properties of three common molecular biology...exonuclease, polymerase, and ligase. In Gibson assembly, DNA fragments with 20–40 base-pair homology at...benefit of Gibson cloning is that it allows for the assembly of multiple fragments of DNA in the chosen orientation...backbone. A drawback of this system is that Gibson assembly works best when combining DNA fragments over 200...custom overhangs that can then be efficiently assembled together. The advantages of type IIS systems are...

  19. Optogenetics Guide

    Type
    Guide
    ...attaching only one member of the Zdk/LOV2 pair to the target protein, and the other to the membrane where the ...trafficking signals to localize opsins to the cell membrane. Microbial opsins, such as those described below... at physiological pH and trafficks well to the membrane. Excitatory (Depolarizing) ChR Variants. The optogenetic...from Kir2.1 and ER export signal provide improved membrane targeting 589 Archaerhodopsins Arch Light-activated...experimental contexts, including protein activation, membrane localization, and transcriptional activation. ...reversibly sequestering targets into large, optically assembled protein architectures in living mammalian cells...light-inducible dimers; Bacterial SsrA peptide is embedded in the LOV2 domain (iLID plasmid), while its natural...

  20. Antibody Guide

    Type
    Guide
    ...Transfer proteins from the gel to a membrane. Incubate the membrane with an unconjugated primary antibody...block the membrane to remove excess antibody and prevent unwanted binding. Incubate the membrane with a ...Wash and block the membrane. Activate the HRP with a substrate kit. Image the membrane using either X-ray...tears, saliva, mucus) where it protects mucosal membranes Dimer with a valency of 4 IgA1; IgA2 IgD Coexpressed...short peptide linkers. Diabodies can be used for assembling protein nanostructures. Figure 2: An antibody...recognizes your primary antibody source species. Remember that recombinant antibodies will also have a source...
Showing: 601 - 620 of 629 results