...every project we undertake and every service we provide is tested against fulfilling our mission. “Addgene...continuously improve all the extra services we provide like quality control, curation, technical service...starting new initiatives as opposed to fundraising.   Aside from enabling us to make all decisions with the ... keeping excellent records and maintaining confidentiality where it is appropriate. This grows our relationship...Addgene increases productivity by providing world-wide access to curated, high-quality data and reagents...processes, distribution, and customer service) Addgene provides unmatched customer service that is consistently...

...middle of the targeted region, as shown below.  To identify optimal nickase designs, IDT scientists Mollie...insert an EcoRI site using a single-stranded oligonucleotide (ssODN) donor. Using a D10A nickase to insert...nickase outperformed both WT Cas9 sites tested by a wide margin, exhibiting >20% repair efficiency. Clearly... is especially useful if there are no available guides close to the desired mutation site. Quick tips...small insertions or tags (ssODN; IDT Ultramer® Oligonucleotides) Use 100 nt homology arms for large insertions... Find CRISPR Nickase Plasmids Browse the CRISPR Guide ...

...but overwhelmed; I had no idea where to start. Thankfully, my managers set aside time for me to complete...the lab! I got to assist production on a protocol video. Don't worry - no pipettes were harmed in the making...allowed me to succeed. As a team, we talked through slide design, discussion items, big takeaways, and timing...depositor welcome kits, note taking for protocol video production, and keeping inventory of our merchandise...talk to. This six month experience made me more confident in myself, my abilities, and my future - something...

... at the Addgene Science Career Guide eBook Addgene Science Career Guide eBook.   Resources Resources... a master? Here are a few questions to help you decide if a master’s might be for you. 1. Are you interested...path one you could see yourself taking? If so, consider setting up an informational interview with them...your interests and career goals. You might still decide to earn a PhD, but if I had to do grad school over...eBooks from Addgene, including the Science Career Guide\  ...

...an introduction to plasmids. Plasmids 101 will provide you with an overview of general molecular biology.... Our mission is to curate a one-stop reference guide for plasmids, so that you can spend less time researching... such as yeast and plants. In nature, plasmids provide one or more functional benefits to the host such... biologists to broadly utilize plasmids across a wide range of applications. Some common plasmid types...scientists can track their location or quantity inside a cell Plasmids 101: Green Fluorescent Protein...references, and how to maintain your plasmid stocks. The guide also contains multiple protocols and troubleshooting...

...pour over the sides.  These tiny gels and tiny lanes required less agar, less ethidium bromide, less stop...discards according to the Cambridge, Massachusetts guidelines and wear an extra sweater so we don’t have to...smaller gel apparatus until I settled on using glass slides. With just the right amount of agarose, I could...could pour a very thin gel on a large glass slide using only surface tension.  It's a bit tricky to pour ...plastic and solder this thing together.  One of the downsides—there was no cover so you had to be careful not...

...in 2012. Using structural modeling and structure-guided direct evolution, Nathan Shaner and colleagues ...respectively. mNeonGreen can be used as a fusion tag for a wide range of applications from traditional imaging to...resolution imaging. mNeonGreen also has little sequence identity to other common fluorescent proteins and therefore...The team that developed mTFP1 further sought to identify why this protein was blue-shifted compared to ...blue-shifting more than others. During this process, they identified mWasabi, a bright, monomeric green fluorescent...variants of Clavularia cyan fluorescent protein: identification of the molecular determinants of color and ...

... sequence identity was a different story - the team found that the percent sequence identity between the...) and comparative properties (percent sequence identity and percent query coverage) that could be determined...synthetic or natural. They saw that a percent sequence identity below 85% correlates with synthetic sequences.... The researchers then used their classifier to identify synthetic and natural sequences in Addgene’s database...have practical applications in monitoring the accidental or intentional release of engineered organisms...

...per month in November 2015. We will continue to provide you with more fascinating, informative, and actionable...technology in their own labs. We’re overjoyed to provide you with these fantastic educational resources ... you’d like to see on the blog or to contribute ideas for your own guest posts by e-mailing us at blog...inversions, deletions, and translocations. It also provides researchers with many different ways to control...the right one. In this post, Addgenie Melina Fan guides us through the basics of the validation process...experiment? In this post, Addgenie Chari Cortez guides us through some common controls and why you might...

...enough to have the funding and the resources to provide them to their students. My desire to create a set... cell-free (FD-CF) components. Making proteins outside of a cell - cell free extracts Typically, if you...used in high school classrooms, but it could be widely adapted and used in a variety of settings. If you...are an instructor at the college level, perhaps consider using some elements of BioBits to transition your...researcher interested in adapting your research ideas into hands-on educational activities using the BioBits... Resources on Addgene.org Browse our protocol videos to learn simple lab techniques Check out our molecular...

...stain for 1 minute. Rinse the slide briefly with tap water. Flood the slide with Gram's iodine solution....staphylococci and streptococci. Gram negative bacteria, or diderms, have a thinner peptidoglycan membrane and an outer...safranin counterstain for 30 - 60 seconds. Rinse the slide in tap water until the runoff is clear, blot gently...types. In water, the dye dissociates into CV+ and Chloride (Cl-) ions. When the negatively charged iodine...into a specific bacterium, it can be important to consider the type of bacteria it is being used in, and ...

...interwebs for lab resources, and instead conveniently provide them on your smart phone. 1. Lab Archives by LabArchives...Gibco Cell culture manual and basic instructional videos. The search function in the manual just highlights...with NEB enzymes, the app is quite helpful and provides a list of directions including which buffers to...primer, you only need to skim the alphabet on the side of the screen to find the sequence for your enzyme...explain the properties of or draw an amino acid or peptide for some reason. It contains all twenty amino acids...allow you to write your colleagues’ names in a polypeptide chain, except if their names have a B, J, X, ...

... proteins. Video by ACS. For a more cartoony explanation of R-bodies, check out this video. Most types...by propagating through an enormous lattice of identical subunits. A brief history of R bodies R bodies...proteins and remain functional, 2) break membranes outside of their natural context or 3) be modified to extend...fluorescent protein fusions allowed us to image R bodies inside cells, and these constructs may be useful to future...soluble when extended (see figure to the right), we identified R-body mutants that extend at both lower and ...

... More people in leadership roles leads to more ideas, initiative and creative thinking. The team members... must usually take on all tasks that involve confidential information. No matter who you delegate to, ...can help ensure successful results.  Can you provide the information and resources needed for the assignment...Setting someone up for failure by delegating is a bad idea. How do I make sure it goes well? As with most ...even if it is just 15 minutes a week. Clearly identify constraints and boundaries. Where are the lines...issues do you want to know about right away? Provide adequate support. The manager must be available...

... perhaps surprising, yet important, factors to consider when seeking your dream lab. The principal investigator's...benefits. Therefore, lab selection should focus on identifying a lab that fits both work style and development...development needs. These two factors will provide a solid foundation on which to thrive during time spent as ... factor. The following questions can serve as a guide for the quest of a perfect "fit". How competitive...want a non-academic career path?  If you are considering this, make sure to choose an advisor that supports... into place frequently and you sometimes feel confident in your abilities. You have intense periods of...

...arises when managers fail to set expectations or provide timely feedback, leading to unproductive dynamics... mentors who have made similar transitions can provide you with insights, lessons from their experiences...work. On the other hand, it’s perfectly valid to decide that managing isn’t for you at this point in your...focusing on molecular biology and metabolism. He now guides fellow PhDs in their careers with actionable steps...Coursera, LinkedIn Learning, or edX. Podcasts: HBR IdeaCast Coaching for Leaders More resources on the ...Makes a Good Leader Anyways?  Transferable Skills Guide: Managing a Team  ...

...With this initial success, we will continue to provide and expand this diverse and useful collection of...inventory is based on CRISPR tools, though we do provide a few non-CRISPR related viral preps. Cas9 is the...with a second vector that delivers gRNA, such as lentiGuide-Puro. The alternative single-vector systems typically...gRNA lentiviruses easily made in your own lab. We provide protocols and useful tips for generating your own...exclusively activated by the small molecule clozapine N-oxide (CNO) (Krashes et al., 2011). Viruses expressing..., Shalem O, Zhang F. Improved vectors and genome-wide libraries for CRISPR screening. Nat Methods. 2014...

...impact both translation and fidelity of translation, leading to system-wide effects. And on top of that...modification, or add in fluorescent or chemically reactive side chains, all of which can help determine structure...reagents accessible to your lab. The solution GCE provides almost endless possibilities for researchers, ...researchers can easily implement in their own labs. They provide resources, knowledge, and most importantly, validated...that is by using Addgene’s Blue Flame award to identify highly requested GCE plasmids. But they’d also...

...through our hot plasmids articles. These articles provide brief summaries of recent plasmid deposits and ...to prevent the incorporation of non-specific nucleotides into the dsRNA leading to increased RNAi efficiency...This tetramer cleaves chlorophenol red-b-D-galactopyranoside on the assay paper turning it purple, allowing...30370338.  SH3 domain collection reveals novel peptide ligand motifs Article contributed by Michael Lemieux...specificity landscape of the SH3 proteins using peptide-phage display and deep sequencing, revealing that...protein stability, small molecule binding screens, peptide arrays, and phage display selections. In addition...

...biotechnology that are ripe with opportunity. We’ve decided to jump into one of those unexplored areas with...food, not medicine, and not mandatory – people can decide for themselves if they want this beautiful flower...begun to pivot towards plant biotechnology and provide a much needed boost to basic molecular plant research...are well understood. Additional work has also identified and characterized mechanisms and components of...you to our guest bloggers! Nikolai Braun grew up outside of Washington D.C. and has always enjoyed exploring...laboratory and received her M.S. from CSU in 2014. Outside of the lab, Keira enjoys spending time with her...