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We narrowed to 1,035 results for: LAS

Showing: 601 - 620 of 1035 results

  1. Important Considerations When Using AAVs

    Type
    Blog Post
    ...demonstrated that certain serotypes can undergo axoplasmic transport. These AAVs may move toward the cell...be easily performed too. While I implied that axoplasmic transport of AAVs is a potential problem in many...Ready-to-Use AAV Preps Browse All of Our Viral Plasmids and Resources ...

  2. Custom CRISPR Screens & the Green Listed Software

    Type
    Blog Post
    ... a few gRNAs suggested to be effective by world class CRISPR labs.  Check Out This Video to See How Green...particular, one slide of the presentation made a lasting impression on me. The slide showed a complex signaling...Find CRISPR Screening Libraries Browse All CRISPR Plasmids ...

  3. Fluorescent Proteins 101: Photoactivatable Fluorescent Proteins

    Type
    Blog Post
    ... models perfer the PA-RFPs over PA-GFP. Another class of irreversible PA-FPs are the photoconvertible ... (level of zoom, intensity, possible excitation lasers) it is also important to optimize your activation...Mizuno, and Atsushi Miyawaki. "Regulated fast nucleocytoplasmic shuttling observed by reversible protein highlighting...

  4. Illuminating Choices: A Guide to Selecting Fluorescent Dyes and Ligands

    Type
    Blog Post
    ...mitochondria, and different dyes can result in varying cytoplasmic background signals when used with the same protein...cell and protein analysis products. You can find plasmids deposited by Promega here.    References and ...More resources from Addgene Fluorescent protein plasmids and resourcesA practical approach to choosing ...

  5. A Primer on Optogenetics: Introduction and Opsin Delivery

    Type
    Blog Post
    ... making fiber optic cables only to hook up your lasers and not have the proper controls or a proper behavioral...surgeries. Identify coordinates through a rodent brain atlas or research papers and practice with die injection...PMC3314244. Resources at Addgene Find optogenetics plasmids! Find AAV resources! Find fluorescent protein ...

  6. Antibody Validation for Flow Cytometry

    Type
    Blog Post
    ... (tagged or untagged) protein When expression plasmids are available, or the necessary expertise to clone...involves the transient transfection of expression plasmids in a given cell line. For flow cytometry, it is...2021). TGFβ1 induces resistance of human lung myofibroblasts to cell death via down-regulation of TRPA1 ...

  7. Viral Vectors 101: Systemic Capsids

    Type
    Blog Post
    ...about that collection. Table 1: Systemic capsid plasmids available from Addgene Capsid Published ...re interested in. Then request your capsid (or plasmids) and let the real work begin — validation, validation...2021). Enhancer viruses for combinatorial cell-subclass-specific labeling. Neuron,109(9), 1449–1464.e13... P. (2021). Functional enhancer elements drive subclass-selective expression from mouse to primate neocortex...

  8. It’s CRISPR Week at Addgene!

    Type
    Blog Post
    ...received the first CRISPR plasmid deposit. Now we have over 8,000 CRISPR plasmids in the repository that ...

  9. #Deckthelab Contest Winners 2017

    Type
    Blog Post
    ...Deckthelab contest. The bar was set very high from last year’s impressive entries, but our community of ...2017 #Deckthelab winners. We hope everyone had a blast decorating and creating memories with their labmates...

  10. Twenty Years of Sharing Science!

    Type
    Blog Post
    ...trying.  When we started in 2004, we were the plasmid repository. In 2016, we added viral vectors, and...addgene.org! Fun fact! Did you know you can search for plasmids from a publication by pasting the paper's title...

  11. New Educational Resource: The Addgene Videos Page

    Type
    Blog Post
    ...topic or lab process. Along with our role as a plasmid repository, Addgene aims to be a vital educational...Addgene.org Check Our Our Protocol Pages Browse our Plasmid Reference Pages ...

  12. Celebrating the 2024 Nobel Prize in Chemistry

    Type
    Blog Post
    ...Baker, who was awarded the Nobel Prize in Chemistry last week alongside Demis Hassabis and John Jumper! The...evolution. The Baker Lab has deposited multiple Top7 plasmids at Addgene, along with other tools like high resolution...

  13. Addgene Protocols Go Viral: AAV Purification and Titration

    Type
    Blog Post
    ...yield and quality of the viral preps. For example, last year we published an article describing a sucrose...instructions and calculations on how to prepare a plasmid stock used to generate a standard curve, visit ...

  14. Antibody Data Repositories and Search Engines

    Type
    Blog Post
    ... Data repository From the Human BioMolecular Atlas Program (HuBMAP)  Antibody Characterization ...imaging Data repository See blog post mAb3D Atlas Mouse and human brain  IHC Data repository...

  15. Editor's Choice, October 2016

    Type
    Blog Post
    ... Read All of Our Editor's Choice Blog Posts Last month there was plenty of excitement at Addgene. Our...myself and two of my previous graduate school classmates, Ben Vincent and Niroshi Senaratne. When we recorded...

  16. Congratulations, Deck The Lab winners!

    Type
    Blog Post
    ... Congratulations to the winners! Deck the Lab: Classic Edition Winners In a tie for first place, the Vestibular...DeckTheLab and continue the tradition we started last year: The Christmas Decoration Competition! #ScienceFun...

  17. Performing In Vivo CRISPR Screens Using the FITS Approach

    Type
    Blog Post
    ...FITS deposit also includes pLX_305_Ovalbumin, a plasmid that enables introduction of the model antigen,...to study antigen-specific responses. Find FITS plasmids here! Marty LaFleur is a Postdoctoral Fellow in...
Showing: 601 - 620 of 1035 results