...binding of a new inactive heterotrimeric G protein complex and subsequently another round of signaling.    ...finally, to a response? Let’s take a look at a few examples with plasmids deposited at Addgene.  PRESTO-TANGO...gamma subunits of the heterotrimeric G protein complex that can be used to detect G protein activation...

...students focus on the application process, and simplify access to academic resources, we created STEAMid...worried about any eligibility provision, don’t guess. Simply reach out to the program coordinator with your ...Chemical Biology Program at Harvard. Roo wants to simplify access to academic resources among economically...

...transfection step of the viral packaging cell line. For example, Phoenix, a second generation γ-retrovirus packaging... to enter the nucleus through the nuclear pore complex. This feature may be useful for the transduction... the components found within the backbone. For example: pLXSN has the wild type LTR, an MCS for cloning...

...cool because they can transmit information, for example, from one of the 180 regions to another or from...the brain to the rest of the body. Therefore, by simply looking at how neurons are physically connected...even further defined population of neurons. For example, say I wanted to access all the neurons that signaled...

...applications, while electroporation of a human, for example, isn’t a reasonable option. Since viruses used...infect both non-dividing and dividing cells. They accomplish this through nuclear localization signals which... immunodeficiency virus type 1 preintegration complexes.” Proc. Natl. Acad. Sci. U.S.A. 89 (1992) 6580...

...chromosome your transgene has integrated. This can be accomplished with a few matings to balancer chromosome lines...throughout the lifespan of the fly to determine the implications of your genetic manipulations. You can produce... (2). Metabolic assays such as Seahorse can be employed to look at oxygen consumption. Drosophila are ...

...that are related to the imaging strategy and the sample. Practical brightness A protein with a high intrinsic... future application. In figure 2 we provide an example of the comparison of the practical brightness of...to this data. The practical brightness assay (employing co-expressing fluorescent proteins or using endogenous...

...2004. We’ve already distributed over 500 viral samples to scientists all over the world. Now that the ...donations. That seed money was used to build a completely self-supporting service so that Addgene is not..., tracked production and distribution of viral samples. The Office Team made sure we could ship internationally...

...intensive to process a large number of microbiome samples. To enable large-scale, longitudinal studies of...and can approximate mRNA concentration within a sample. Using this system, the Collins lab was able to...lab validated their platform on clinical stool samples by comparison to qPCR and demonstrated that it ...

...necessary” detail after “necessary” detail and end up completely overwhelming your audience - like the professor...intuitive that actually wasn't. Maybe I needed to employ a visual aid instead of describing something verbally...group was just really tired and needed a slower, simpler presentation than I had planned; other times students...

...several different species are present. One common example is using pre-adsorbed secondary antibodies (which...antibodies are polyclonal, as their function is to amplify the signal from a primary antibody; the ability...target protein thereby greatly increasing signal amplification.  When to stay away from polyclonal antibodies...

...show. After graduating with a master’s, Susan completed a science writing internship with the National...planning.” To beef up her resume, she decided to complete a certificate in Strategic Communications and ...the most satisfying parts of the job is having completed one of these difficult pieces: “It’s probably ...

... the LoxP sites, which is more difficult than a simple knock out. Looking for some permanent knock out...two varieties: tet-on and tet-off. As the names imply, tet-off turns off constitutively expressed proteins... tunable control These systems can be easily implemented for exogenously introduced proteins, as many ...

... practice to compare degradation rates between samples treated with or without an autophagy inhibitor....accumulates in the vacuole. See Figure 2 for an example of what fluorescence looks like for cytoplasmic...Fluorescent Protein Oligomerization Seeing Red: Simple GFP Photoconversion Light Sheet Fluorescence Microscopy...

...preserves RNA structure and naturally occurring complexes present in the cell, it can also introduce non-physiological...non-physiological RNA-protein interactions due to sample processing. Require prior knowledge that the protein...levels seen in HHCS patients. These are just three examples of how RaPID can be used to study RNA-protein ...

...the take rate of mouse xenografts after cell implantation. Their goal was to figure out how to optimize...barcodes in the population (all barcodes can be amplified using the same sets of forward and reverse primers...dynamics in response to cancer therapy using high-complexity barcoding." Nature medicine 21.5 (2015): 440-...

...treatments, but are not useful for genetic engineering. Examples of outbred strains include Swiss Webster and Harlan... are injected into a blastocyst which is then implanted into a female animal for gestation. Nucleases ...are microinjected into a zygote, which is then implanted into a female mouse for gestation. All three can...

... the xanthine produced via complete demethylation of caffeine. For example, the Barrick lab was able to...living biosensor for measuring caffeine content in samples. The Barrick lab and the UT Austin undergraduate...

...animal models of disease and cultured cells. For example, in 2013, Jinsong Li and colleagues chose to explore...trials is somewhat similar (Hussein et al). It employs Cas9 to cut at two sites within the viral genome...current delivery technologies are suited for. For example, lipid nanoparticle delivery tends to be trafficked...

...In order to understand the complex wiring of the brain and the underpinnings of neurological disease,...while resulting in minimal off target effects. For example, if your receptors are expressed in the brain, ...receptor acts through. The DREADD hM3D(Gq), for example, lowers the threshold for neuronal signaling and...