We narrowed to 898 results for: NSI;

... toolkit from Benjamin Field’s lab. Special Considerations There are a few special exceptions to the usual...constructs or certain cell environments). Also consider that a protein fusion may disrupt functionality...

...   Let me leave you with a few practical considerations: Design your gRNA to overlap the CpG of interest...interest. Having the CpG directly inside the gRNA sequence is a great guarantee that DNMT1 will be blocked...

...abundance of your targets isn’t too different, consider antibody affinity: stronger binding antibodies...membranes recommend re-wetting with methanol before rinsing with wash buffer, as dry PVDF won’t evenly re-absorb...

...the ‘quality’ of the gRNA sequence, including propensity for off-targets, secondary structures, etc., ...more pan-genome PAM flexibility, you’ll want to consider one of several Cas9s that recognize NG PAMs. The...

... crossing the blood-brain barrier.  Another consideration when picking ligands for chemogenetic studies...response you aim to manipulate. It is important to consider that the same receptor can elicit different responses...

...Polyclonal antibodies are produced in animals and consist of a mixture of antibodies recognizing many different...hybridomas.  First, the long-term stability, consistency between batches, and molecular definition of ...

... example, the CAG promoter, a hybrid promoter consisting of a CMV enhancer, chicken beta actin promoter...any template DNA and primers.  After performing extensive QC on that sample, we’ll then load it on to our...

...in different fields that can provide different insight into an area - not just working within the established...collaborators can also help you get some critical insight into what you're doing wrong. All this helps to...

...using bacterial phage vectors with CRISPR/cas9 sensitizing gene cassettes. Thse vectors are engineered to...genes in the bacteria thus making the bacteria sensitive to known antibotics. These might be used on hospital...

...strengths and work effectively. Leadership responsibilities Being a leader in an organization, you need... different hats and came to understand the responsibilities that come with leadership. The NPA was a great...

... a company. Revolution Bioengineering is two responsible adults in their 30s who have quit their academic...below. We’re happy to answer questions about this transition (so far) and what we’ve learned.  Thank you ...

..., cultures treated with a folA gRNA were more sensitive to trimethoprim when compared to control gRNA ...for trimethoprim, there would be no changes in sensitivity. Pooled knockdowns are also possible with Mobile-CRISPRi...

...primarily found in the culture media, you should consider performing PEG precipitation. Instead of sonicating...solutions are carefully layered and then the viral suspension generated during AAV production is overlaid. ...

...normally lies around 7.35-7.4 so mammalian cells are sensitive to pH. Anything between orange and light orange...Additional passages will allow your cells to achieve a consistent growth rate. For every minute spent organizing...

... 3-D path of the protein’s backbone in three-dimensional space, but their flexible and jiggly nature suggested...structure into experimentally determined electron density, have been known to say something silly like “See...

...which way your population needs to be shifted, consider your perpendicular lines. In this case, the population...applying very large compensation values (100+), consider what two markers your fluorescent markers are ...

... increase efficiency of these systems, though consistent expression can still be a challenge. A multitasking...powerful than it is. The nuclease domain of Cas3 is considered weak, especially compared to the power of Cas9...

...epitope, a phenomenon known as sequence context sensitivity (Schüchner, et al. 2020). In their study, some... newer Myc-tag antibodies appeared to be less sensitive to sequence context.    So, do Schüchner, et al...

...nuclease-null—in order to effect different perturbations. Consider, for instance, the challenge of attempting to ...alterations can be exploited to quickly and inexpensively generate a potent Cas9-based transcriptional...

...can survive positive selection, but it will be sensitive to negative selection. Remove the positive selection...creating knockout animals or cell lines, please consider sharing them by depositing with Addgene! Figures...