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We narrowed to 966 results for: OMP;

Showing: 601 - 620 of 966 results
  1. Plasmids 101: Antibiotic Resistance Genes

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    Blog Post
    ...labs today are semi-synthetic or fully synthetic compounds. Antibiotics can be categorized based on whether...gene of interest is cloned into/inactivates or completely removes (in the case of Gateway cloning) one ...make sure your plasmid and E. coli strain are compatible! Check out this list of common E. coli genotypes...
  2. CRISPR Challenges: Standardization and Homology Directed Repair

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    Blog Post
    ...common lexicon for genome editing studies. These complementary goals are aimed at improving translation of ... loosely paraphrased in the tweet below. The complexity and challenges of homology directed repair (HDR... I think I may have finally grasped just how complicated DNA repair pathways are. I fully admit to being...
  3. Another Pathway into Cells: iTOP

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    Blog Post
    ...of their luciferase assay, they concluded that components in the buffer used for protein purification must...percentages (78-79% after two rounds of transduction.) Compared to CCP transduction, iTOP is at least four times...Astolfo et al. were eager to see if iTOP would be compatible with CRISPR/Cas9 genome editing. Although CRISPR...
  4. Advice for Moving Into Sales After Your Science Postdoc

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    Blog Post
    ... life science researchers. Many biotechnology companies are looking for PhD or Master’s level scientists...have done?” and “Would their academic experience compliment this sales and marketing role?”. Studying for...entrepreneurial bug and has set up an ELISA kit company on whose website you can find some great information...
  5. Pushing the Limits of DNA Assembly

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    Blog Post
    .... Generation of sgRNA libraries, with maximum complexity, is achieved with synthetic oligos with a randomized... genome. Many of these techniques require more complex DNA assemblies and improvements in these methodologies...the limitations associated with otherwise more complicated assembly approaches, and opens the door for new...
  6. What Good Citizenship Can Do for Reproducibility in Science

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    Blog Post
    ... It doesn’t have to be this way. We can never completely eliminate the risk that a minor variation in ...Press.  She trained originally in cell biology, completing her PhD at the MRC LMB in Cambridge UK and then... Vice President. Her current responsibilities encompass overall leadership of the Cell Press editorial...
  7. FlipGFP, a novel fluorescence protease reporter to study apoptosis

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    Blog Post
    ...responsible for the degradation of over 600 cellular components ultimately leading to cell fragmentation and ...FlipGFP. FlipGFP increases fluorescence 100-fold compared to other reporters (Zhang et al., 2019). How does...and (3) β-strand 11 (β11). Each part contains components and residues that are necessary for fluorescence...
  8. Revamp Your Lab Meetings With Creative Virtual Collaboration

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    Blog Post
    ...writing and this recent article published in PLOS Computational Biology (Frassl et al., 2018): “Science is increasingly...collaboratively writing a multi-authored paper. PLoS Comput Biol 14:e1006508 . https://doi.org/10.1371/journal.pcbi...Simple Rules for a Successful Collaboration. PLoS Comput Biol 3:e44 . https://doi.org/10.1371/journal.pcbi...
  9. Summer SciComm Series: A PhD in Science Communication

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    Blog Post
    ... I asked participants to read short abstracts comparing the mortality rates between two controversial ...and biased. As a result, the control abstract comparing two fictional medical issues – chronic and acute...community. I brought along printed copies of studies comparing the filtration efficacy of different materials...
  10. R Bodies: Membrane-Rupturing Microscopic Tools

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    Blog Post
    ...sources. While the importance of understanding these complex processes can’t be overstated, we can also learn...degradation under a variety of conditions. They are composed of 4 proteins encoded in a single operon and were...
  11. Resume Writing for Non-academic Science Careers

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    Blog Post
    ...experience using image analysis software. If you use computer programs to predict protein structures, then you... you might know a thing or two about computer modeling. Make sure to focus on these skills. The only exception... into a communications role instead after she completed her MSc in Experimental Medicine. She is the Community...
  12. Plasmids 101: How to Verify Your Plasmid Using a Restriction Digest Analysis

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    Blog Post
    ...than a day. Diagnostic restriction digests are comprised of 2 separate steps: 1) incubating your DNA with... construct. Consider buffer and temperature compatibility when digesting with more than one enzyme. Consult...will allow you to approximate the DNA mass of comparably intense samples of similar size. Run the gel ...
  13. Addgene’s Viral Service - Why Virus? Why Now?

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    Blog Post
    ...service supporters We would like to thank the companies who supported this project with funding. Alnylam...donations. That seed money was used to build a completely self-supporting service so that Addgene is not...distribution, this project involved every Addgenie in the company. Our Finance, Business and Legal teams had to pave...
  14. Developing a cold tolerant E. coli using specialized chaperones

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    Blog Post
    ...However, one of the many proteins which are not compatible with regular mesophilic systems are the psychrophilic...chaperones protein resulted in higher levels of growth compared to the E. coli strain expressing the empty vector... Medal in the Undergrad category at the iGEM Competition 2019. He has an active interest in scientific...
  15. OCaMP: A New Calcium Indicator for Neural Imaging

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    Blog Post
    ...needs to emit a different color of light and be compatible with the lasers available in your microscope ...jRGECO1a and jRCaMP1a are widely used as well. To complement these tools, we recently developed a new orange...1030 nm, enabling two-photon imaging without compromising on brightness, ∆F/F0, or signal-to-noise ratio...
Showing: 601 - 620 of 966 results