Plasmids 101: Protein tags
Type
Blog Post
...deciding on which one to use will depend on your application (see table below). The antibodies available for...
We narrowed to 663 results for: PPL;
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To Codon Optimize or Not: That is the Question
TypeBlog Post...synonymous codon usage bias, and its potential applications. Nucl Acids Res 15:1281–1295 . https://doi.org... -
The PAM Requirement and Expanding CRISPR Beyond SpCas9
TypeBlog Post...to dramatically increase the number of CRISPR applications. The first non-Cas9 CRISPR protein adapted for... -
Save Time with Transient Plant Leaf Transformations
TypeBlog Post...placed in an Agrobacterium solution and a vacuum is applied with a vacuum bell or desiccator. Successful infiltration... -
Beyond the Blood Brain Barrier: PHP.B Enters the Central Nervous System Via the LY6A Receptor
TypeBlog Post... interested in using AAV-PHP.B vectors? See Supplementary Table 3 in Huang et al. for a list of PHP.B ... -
CRISPR-mediated Plant Base Editors
TypeBlog Post...question probably comes to mind: can CRISPR be applied to fix the defective base precisely? Until 2016... -
CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?
TypeBlog Post...Motif (PAM), the sequence NGG. If these conditions apply to your experimental target, SpCas9 and its enhanced... -
Viral Vectors 101: Pseudotyping
TypeBlog Post...Wickersham et al., 2007). This system requires first supplying rabies glycoprotein along with the TVA receptor... -
Controlling for Off-target Effects with a New Genome-wide CRISPR Screen Design
TypeBlog Post...noise, of dsDNA breaks caused by CRISPR. See supplemental figure 6 for more details. Safe-targeting guides... -
Using Video to Share Your Science: We Share Science
TypeBlog Post...private sector researchers, or pretty much anyone applying the scientific method to a question. Videos from... -
Changing Labor Laws Bring Increased Postdoc Wages
TypeBlog Post...institutions, or institutions they're thinking about applying to, are doing - for example, your benefits may... -
The Challenges of Cell Culture
TypeBlog Post...including GSK, AstraZeneca-MedImmune, and Infors & Applikon. Due to his high level of experience he now travels... -
"Hall of Fame" AAV Enhancers from the Allen Institute for Brain Science
TypeBlog Post...when discussing raising children, it can also be applied to research. Sharing knowledge and tools is the... -
Tips and Tricks for Using Golden Gate Modular Cloning (MoClo)
TypeBlog Post...Modular Cloning Plasmids 101: Modular Cloning Applications and Kits Plasmids 101: Golden Gate Cloning ... -
Plasmids 101: Shuttle Vectors
TypeBlog Post...vector, the deficient cells are grown in media that supplies the key nutrient. The vector provides the missing... -
CRISPR 101: Any Base Transversion Editors
TypeBlog Post...researchers can now select the best tool for their application from among many options. Conclusion With these... -
Twenty Years of Sharing: Addgene's Viral Vector Service
TypeBlog Post...viral vector service in 2016. We realized we could apply the lessons learned from our plasmid lab to develop... -
Mesothelioma - Causes, Symptoms, and Treatment
TypeBlog Post...pemetrexed and cisplatin –because it cuts off the blood supply to fast-growing cancer tumors. In fact, the use... -
Avoiding the Dark Side of Fluorescent Protein Fusions with mOX FPs
TypeBlog Post... of a mystical "Force," this quote is equally applicable to many microscopy experiments with fluorescent... -
Plasmids 101: E. coli Strains for Protein Expression
TypeBlog Post...cell-density they support; however, minimal media supplemented with M9 salts may be preferable if the protein...