We narrowed to 921 results for: Por

1. Hot Plasmids and Viral Preps - July 2021

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   Blog Post

   ...straightforward workflow for doing gene targeting, reporter generation, controllable gene knockouts, and inducible... models, toolkits like this one provide new opportunities for investigating genetic mechanisms related...

2. Chemogenetics vs. Optogenetics: Which Method Should I Choose?

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   Blog Post

   ...   Timing Optogenetics offers a high degree of temporal resolution as receptors can be turned on and off... meet these requirements. TL;DR If precise spatiotemporal control of a small subset of neurons is necessary...

3. Genetically-encoded Sparse Cell Labeling - A SPARC of Innovation

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   Blog Post

   ...was developed by Thomas Clandinin’s lab, and incorporates several common genetic tools in Drosophila (...: a method to genetically manipulate precise proportions of cells. bioRxiv 788679; https://doi.org/10.1101...

4. The Open Repository of CRISPR Screens: CRISPR Screen Data in One Place

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   Blog Post

   ...screen results. Screenshots updated 2024. Importantly, metadata for each screen is captured by BioGRID...also captured as metadata.   Screen results are reported as the original quantitative gene-level scores...

5. Early Career Researcher Toolbox: Free Tools for Making Scientific Graphics

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   Blog Post

   ...ve realized my figures are just as important, if not more important, than my writing. Initially I didn...

6. Viral Vectors 101: Parts of the AAV Packaging Plasmid

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   Blog Post

   ...capsid, “pumps” the genome into the capsid through a pore in the capsid. Rep proteins are also involved in...2020) Adeno-Associated Virus Genome Interactions Important for Vector Production and Transduction. Human ...

7. Addgene's a Nonprofit? Nonprofit Awareness Day 2017

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   Blog Post

   ...do. From all of us to all of you. Thanks for supporting nonprofit, open science and Addgene in our mission...Collaboration with Fellow Nonprofit Seeding Labs Addgene Supports Rare Disease Research Listen to Our Interview ...

8. CASTing Off for New Shores in Human Genome Editing

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   Blog Post

   ...bacterial genome engineering tools, with early reports of 40%–60% efficient integration (Klompe et al....streamlined, one-size-fits-most approach. evoCAST is an important step toward that kind of gene treatment. The researchers...

9. Antibodies 101: Fc Effector Functions

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   Blog Post

   ...starting a cascade of events that results in the pores being opened in the pathogen’s cell membrane (Dunkelberger... when holes are punched in their membranes, the pore'd pathogens quickly lyse and die.  Antibody dependent...

10. CUT&RUN: An Improved Method for Studying Protein-DNA Interactions

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    Blog Post

    ...MNase to antibody bound proteins (Figure 1). Importantly, since Protein A has weaker affinity for mouse... rather than to rely on nuclei extraction. They report that this change offers a significant improvement...

11. Plasmids 101: Walkthrough of Addgene’s Snapgene-Powered Quality Control Process

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    Blog Post

    ...for plasmid quality control are: To identify important plasmid features To align NGS sequencing results...Sanger sequencing at Addgene. By using Snapgene’s “Import primers from a list” function, we can detect all...

12. ReFigure: Save Scientific Figures into Dashboards and Share Your Insights

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    Blog Post

    ...your data to a repository (Figshare is currently supported by ReFigure beta) you can include it in a ReFigure... ReFigure team at [email protected] Please support ReFigure by creating ReFigures and answering this...

13. Oh, The Places You Can Go: Careers in Science Communication - Curriculum Development

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    Blog Post

    ...hands-on science lab kits and digital curricula to support them. Caitlin’s take on her job as an SME: “I write...communicators: “My recommendation would be to take any opportunity in the lab or outside the lab: write!” Caitlin...

14. Oh, The Places You Can Go: Careers in Science Communication - Science Writing

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    Blog Post

    ...He debated taking a position as an application support/sales representative in industry or a postdoc in...-on science lab kits and digital curriculum to support them. Many thanks to Hans for taking the time ...

15. SunTag and Fluorescent Imaging

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    Blog Post

    ...nuclear localization signals (NLS) increase protein import into the nucleus. In developing their SunTag technology...protein function, thereby creating a single-molecule reporter of intracellular processes. Initially, Tanenbaum...

16. Three Tips for Preventing Viral Plasmid Recombination in Your Samples

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    Blog Post

    ...Working on the scientific support team at Addgene, I get to see everything that can go wrong with a plasmid...Growth Conditions To reduce recombination, it is important to not overgrow the plasmid at 37°C. Some researchers...

17. Antibody Neutralization Response Against Pseudoviruses Expressing SARS-CoV-2 Spike Protein Variants

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    Blog Post

    ... assay, the pseudoviruses express a luciferase reporter which is used as a readout for infection of ACE2...neutralize the virus,” says Balazs, emphasizing the importance of the second vaccine dose in mounting a robust...

18. Addgene Receives NIH BRAIN Initiative Grant to Create Open-Access Recombinant Antibody Resource

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    Blog Post

    ... new services. “We appreciate NIH’s vision and support for this project. This grant will allow Addgene... part of this initiative! The project will be supported by a NIH BRAIN Initiative grant (NS119916). The...

19. Bright Monomeric Fluorescent Proteins: mNeonGreen, mTFP1, and mWasabi

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    Blog Post

    ...enhanced GFP (EGFP)-type termini. mNeonGreen was reported as the brightest monomeric green or yellow fluorescent... or for live cell imaging of fusions and FRET reporters. mTFP1 is also insensitive to physiologically ...

20. Intro to Our Summer SciComm Series!

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    Blog Post

    ...through manuscripts, seminars, or posters. It is important to note that it is the actual communication itself...quickly!  The question   The first, and most important question, you should ask for any science communication...