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...Neuromodulators like dopamine and norepinephrine have important functions in the brain but have been difficult... are blocked by alpha1-AR antagonist. This is important because alpha2-AR can provide inhibitory feedback...

...using the BE4max architecture. The new editors reported in this study have ~10-100-fold lower average ... gRNA multiplex. Read the paper in Scientific Reports Find the plasmids Enhanced CRISPR-based DNA demethylation...

...physical scientists are underrepresented. It’s important to note that these results could be biased for...domains more often than other scientists   Figure 5: Portion of Tweets with Scientific Domains, Categorized ...

...through the ‘front door,’ the homepage is still important. It needs to express value, clearly communicate... what a site offers to their users, and most importantly, allow those users the ability to quickly access...

...transgene cargo capacity, enhancing anterograde transport efficiency, and engineering viral capsids that...openness and sharing in science.  We’re excited to support the neuroscience community by sharing not only ...

... interests. We do this by promoting citation, supporting sophisticated search, and curating plasmid collections...other resources. If you think we’re missing an important collection or if you think a particular plasmid...

...reagents. "We are proud to be able to bring such an important collection to the scientific community," says ...scientists, and the FSU team who have made this large, important plasmid deposit possible.   ...

...Addgene's Europe office no longer handles customer support inquiries. The office is now focused on scientific... first to be located in London and focused on supporting European scientists. Born a french frog, I moved...

... and tricks Wang offers this tip: “It's quite important, at every step of the process, that you are not...not losing library representation.” It’s also important that you infect enough target cells. What’s “enough...

...recommends that you sequence any portion of the plasmid that is important for your experiments. There are... Digest Introduction Sequence verification of important plasmid features (such as the gene/insert, fusion...sequences the plasmid to verify tags, mutations and a portion of the insert, but we do not sequence the entire...an example of a trace file from a high-quality portion of a sequencing reaction: Sequence near the beginning...unreliable. This is an example of a trace file from a portion of a sequencing reaction with high background: ...

...starting a cascade of events that results in the pores being opened in the pathogen’s cell membrane (Dunkelberger... when holes are punched in their membranes, the pore'd pathogens quickly lyse and die.  Antibody dependent...

...bacterial genome engineering tools, with early reports of 40%–60% efficient integration (Klompe et al....streamlined, one-size-fits-most approach. evoCAST is an important step toward that kind of gene treatment. The researchers...

...straightforward workflow for doing gene targeting, reporter generation, controllable gene knockouts, and inducible... models, toolkits like this one provide new opportunities for investigating genetic mechanisms related...

...   Timing Optogenetics offers a high degree of temporal resolution as receptors can be turned on and off... meet these requirements. TL;DR If precise spatiotemporal control of a small subset of neurons is necessary...

...was developed by Thomas Clandinin’s lab, and incorporates several common genetic tools in Drosophila (...: a method to genetically manipulate precise proportions of cells. bioRxiv 788679; https://doi.org/10.1101...

...ve realized my figures are just as important, if not more important, than my writing. Initially I didn...

...do. From all of us to all of you. Thanks for supporting nonprofit, open science and Addgene in our mission...Collaboration with Fellow Nonprofit Seeding Labs Addgene Supports Rare Disease Research Listen to Our Interview ...

...nuclear localization signals (NLS) increase protein import into the nucleus. In developing their SunTag technology...protein function, thereby creating a single-molecule reporter of intracellular processes. Initially, Tanenbaum...

...MNase to antibody bound proteins (Figure 1). Importantly, since Protein A has weaker affinity for mouse... rather than to rely on nuclei extraction. They report that this change offers a significant improvement...

...for plasmid quality control are: To identify important plasmid features To align NGS sequencing results...Sanger sequencing at Addgene. By using Snapgene’s “Import primers from a list” function, we can detect all...