We narrowed to 928 results for: Por
-
TypeBlog Post...reagents. "We are proud to be able to bring such an important collection to the scientific community," says ...scientists, and the FSU team who have made this large, important plasmid deposit possible. ...
-
London Calling - Addgene’s New Europe Office in London
TypeBlog Post...Addgene's Europe office no longer handles customer support inquiries. The office is now focused on scientific... first to be located in London and focused on supporting European scientists. Born a french frog, I moved... -
Lentiviral CRISPR Libraries Enable Genome-Scale, Knockout Screening
TypeBlog Post... and tricks Wang offers this tip: “It's quite important, at every step of the process, that you are not...not losing library representation.” It’s also important that you infect enough target cells. What’s “enough... -
CASTing Off for New Shores in Human Genome Editing
TypeBlog Post...bacterial genome engineering tools, with early reports of 40%–60% efficient integration (Klompe et al....streamlined, one-size-fits-most approach. evoCAST is an important step toward that kind of gene treatment. The researchers... -
Antibodies 101: Fc Effector Functions
TypeBlog Post...starting a cascade of events that results in the pores being opened in the pathogen’s cell membrane (Dunkelberger... when holes are punched in their membranes, the pore'd pathogens quickly lyse and die. Antibody dependent... -
Hot Plasmids and Viral Preps - July 2021
TypeBlog Post...straightforward workflow for doing gene targeting, reporter generation, controllable gene knockouts, and inducible... models, toolkits like this one provide new opportunities for investigating genetic mechanisms related... -
CUT&RUN: An Improved Method for Studying Protein-DNA Interactions
TypeBlog Post...MNase to antibody bound proteins (Figure 1). Importantly, since Protein A has weaker affinity for mouse... rather than to rely on nuclei extraction. They report that this change offers a significant improvement... -
Plasmids 101: Walkthrough of Addgene’s Snapgene-Powered Quality Control Process
TypeBlog Post...for plasmid quality control are: To identify important plasmid features To align NGS sequencing results...Sanger sequencing at Addgene. By using Snapgene’s “Import primers from a list” function, we can detect all... -
ReFigure: Save Scientific Figures into Dashboards and Share Your Insights
TypeBlog Post...your data to a repository (Figshare is currently supported by ReFigure beta) you can include it in a ReFigure... ReFigure team at [email protected] Please support ReFigure by creating ReFigures and answering this... -
Oh, The Places You Can Go: Careers in Science Communication - Curriculum Development
TypeBlog Post...hands-on science lab kits and digital curricula to support them. Caitlin’s take on her job as an SME: “I write...communicators: “My recommendation would be to take any opportunity in the lab or outside the lab: write!” Caitlin... -
Chemogenetics vs. Optogenetics: Which Method Should I Choose?
TypeBlog Post... Timing Optogenetics offers a high degree of temporal resolution as receptors can be turned on and off... meet these requirements. TL;DR If precise spatiotemporal control of a small subset of neurons is necessary... -
Genetically-encoded Sparse Cell Labeling - A SPARC of Innovation
TypeBlog Post...was developed by Thomas Clandinin’s lab, and incorporates several common genetic tools in Drosophila (...: a method to genetically manipulate precise proportions of cells. bioRxiv 788679; https://doi.org/10.1101... -
The Open Repository of CRISPR Screens: CRISPR Screen Data in One Place
TypeBlog Post...screen results. Screenshots updated 2024. Importantly, metadata for each screen is captured by BioGRID...also captured as metadata. Screen results are reported as the original quantitative gene-level scores... -
Addgene's a Nonprofit? Nonprofit Awareness Day 2017
TypeBlog Post...do. From all of us to all of you. Thanks for supporting nonprofit, open science and Addgene in our mission...Collaboration with Fellow Nonprofit Seeding Labs Addgene Supports Rare Disease Research Listen to Our Interview ... -
SunTag and Fluorescent Imaging
TypeBlog Post...nuclear localization signals (NLS) increase protein import into the nucleus. In developing their SunTag technology...protein function, thereby creating a single-molecule reporter of intracellular processes. Initially, Tanenbaum... -
Early Career Researcher Toolbox: Free Tools for Making Scientific Graphics
TypeBlog Post...ve realized my figures are just as important, if not more important, than my writing. Initially I didn... -
Oh, The Places You Can Go: Careers in Science Communication - Science Writing
TypeBlog Post...He debated taking a position as an application support/sales representative in industry or a postdoc in...-on science lab kits and digital curriculum to support them. Many thanks to Hans for taking the time ... -
Viral Vectors 101: Parts of the AAV Packaging Plasmid
TypeBlog Post...capsid, “pumps” the genome into the capsid through a pore in the capsid. Rep proteins are also involved in...2020) Adeno-Associated Virus Genome Interactions Important for Vector Production and Transduction. Human ... -
Intro to Our Summer SciComm Series!
TypeBlog Post...through manuscripts, seminars, or posters. It is important to note that it is the actual communication itself...quickly! The question The first, and most important question, you should ask for any science communication... -
Three Tips for Preventing Viral Plasmid Recombination in Your Samples
TypeBlog Post...Working on the scientific support team at Addgene, I get to see everything that can go wrong with a plasmid...Growth Conditions To reduce recombination, it is important to not overgrow the plasmid at 37°C. Some researchers...