...online on Addgene's CRISPR Library Reference page.    References: Genetic Screens in Human Cells Using...as an answer to that question. Additionally, a concurrent Nature Biotechnology article describes the development...GeCKO library, each library has been enriched for different gene targets. These pooled libraries include enrichment...

...compare and analyze any differences between our sequence results and the reference sequences. Using Snapgene...features To align NGS sequencing results with reference sequences To design experiments for further plasmid...Additioinal Resources on Addgene.org Molecular Biology Reference Pages Basic Molecular Biology Protocols Diagnostic...

...Side-by-side comparison of the same experiment from different cell lines/model systems ReFigure allows you ...access life sciences data. You can use ReFigure to coherently save analyses with the relevant figures from ...submitting your data to a repository (Figshare is currently supported by ReFigure beta) you can include it... in a few minutes, visit our YouTube Channel. Currently we can only connect figures from PubMed Central...

...similar purposes, both techniques offer researchers different advantages and limitations.  The four main factors...study. Figure 1: A schematic showing the differences in chemogenetics and optogenetics in a mouse ...manipulated or turned off. In chemogenetics, while different concentrations of ligands can be injected, this... article and sharing their expertise with us. References Urban DJ, Roth BL (2015) DREADDs (Designer Receptors...

...buffer, or some other variations. Does it make a difference? Short answer: yes. This last step will influence...preparation can be eluted in water or in your buffer of preference, and it will be stable for years. Plasmid DNA...choose the best way to store and elute the DNA. References Murakami M (2013) Evaluation of DNA Plasmid Storage... Addgene.org Check out the Molecular Biology Reference Find protocols on plasmid cloning Find plasmids...

...tools for your next experiment! References and Resources References Wood, V., et al., Hidden in plain...this many genes are truly dispensable or are the current identification methods missing something? Here ...an endogenous gene. Each trap uses a slightly different approach (described in detail below), but fundamentally...

...but in time I realized its value. My master’s strengthened my critical thinking and problem solving abilities...along a career path in these two fields looks different however. In industry the career ladder is larger...training can prepare you to present your work at conferences, help with manuscript and grant preparation, ...generalist? Working on a variety of projects and in different fields of research is one of the best parts of...

...RepCap plasmid and its products. Created with BioRender.com   Replication (rep) gene The rep gene encodes... proteins. Alternative splicing generates the different C-terminal regions of the Rep proteins. While ...serotypes are responsible for serotype-specific differences in tissue and/or cell type tropism. Introducing... learn more about the AAV transfer plasmid! References: Naso MF, Tomkowicz B, Perry WL III, Strohl ...

...general primer design, as well as primer design for different cloning applications. Primer3 and Primer3Plus...calculate how much insert and backbone you need for different types of cloning methods, such as restriction ... any cloning method. You can select from five different insert:backbone ratios. In-Fusion cloning molar...Resources on Addgene.org: Read our Molecular Biology Reference Find molecular biology protocols Browse our protocol...

...Open Repository of CRISPR Screens BioGRID ORCS currently houses over 890 CRISPR screens in 629 cell lines...community, but also allows cross-interrogation of different datasets that might otherwise be hard to identify...breast cancer" returns 40 screens performed in 26 different breast cancer cell lines. Screenshot updated 2024...to analyze raw sequence data.  This format is inherently flexible and allows the broadest possible scope...

...pretend that that there aren’t problems with the academic enterprise or that there weren’t some pretty hefty...the News. While research experience is key to my current position, I think it’s largely my work developing...information distillation, etc) are transferable to many different fields. If you start thinking about preparing ...

...transposase has been discovered in the genomes of many different fish species in a variety of non-functional forms... vectors were developed into 16 variants with different selection markers (blasticidin, neomycin, puromycin... each contain two SfiI restriction sites with different overhangs depending upon whether they are on the...only likely to grow in the coming decades.  References 1. Kowarz E, et al. Optimized Sleeping Beauty...

...calcium sensors, different iGABASnFR have been developed and are suited for different applications. The... a few versions of these jGCaMP7 sensors with different properties (for example, overall brightness or...on/off kinetics) that makes them suitable for different applications. We recently released a new variant...

...of autophagosomes. This approach, however, has inherent limitations because it turns a dynamic process...provide an overview of four autophagy biosensors currently available from Addgene. Rosella autophagy biosensor...wavelength, Keima emits at 620 nm. The ratio of signal strength excited at 586 nm to 440 nm serves as a measure...Find the GFP-LC3-RFP-LC3∆G plasmid at Addgene! References Klionsky, Daniel J., Ana Maria Cuervo, and Per...

...pace Constant worry about grants and renewals What is different about non-academia? Scientific publications...interact with scientists just like you What is different about academia? Publishing in peer reviewed journals...especially with peer mentors, to discuss your strengths, weaknesses, interests and passions is another...

...transductions. If you’ve ever wondered how they are different from or similar to the other Ts, we have your ... and with the right safety considerations, they aren't dangerous for controlled lab usage. Using viral...that you can swap out your virus type to achieve different biological outcomes. For example, some viruses...target cells or organism, some primary cells and differentiated cell types are picky when it comes to transfection...

...hiring a scientist. Of course, we have different criteria for different roles but for almost every position...contacting alumni of your lab or university or use your current contacts to ask who they might suggest you speak...effectively is a valuable skill. There are many different kinds of grants that you can apply for. If you...

...Khammash lab can also be found here.   References and Resources References Romano E, Baumschlager A, Akmeriç...functional BLADE variants, too. The team tested different linkers between VVD and the DNA-binding domain... Addgene.org Check out the Molecular Biology Reference Download Addgene's Plasmids 101 eBook ...

... the same. Over the next few years genes from different bacterial (and eventually mammalian) species were...ubiquitous tool that can be diversely applied to many different experiments. Addgene was founded in order to store... keeping with our goal of creating a one-stop reference guide for plasmids we've updated and added new...: 24043817. PubMed Central PMCID: PMC3785787. References: Lederberg, Joshua. "Cell Genetics and Hereditary...

...diverse range of projects at BosLab. Some are entrepreneurs using our space to perform early-stage experiments...yeast transformations! Dennis Oleksyuk, Raphael Laurenceau, and Tami Pudina have initiated a soil microbiome...the data to characterize the microbiomes of the different samples. The goal is to not only learn something...something about the microbial diversity in different soil conditions/locations but to engage the local community...