Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

We narrowed to 1,006 results for: RING

Showing: 601 - 620 of 1006 results

  1. How to Make Friends and Meet People at a Scientific Conference

    Type
    Blog Post
    ...of experimental problems or questions you are pondering and be ready to talk about them. People love to... to help especially when solving problems.  During the conference The conference may start in the airport...post. Stay in touch by liking, commenting and re-sharing their posts. LinkedIn users notice this sort of...

  2. Advice for Starting a Biotech Company from a Grad Student turned Entrepreneur

    Type
    Blog Post
    ...Sample6, for advice on how to take an idea developed during graduate school and turn it into a biotech company...changed, but Sample6 still leverages the core engineering and scientific concepts to engineer bacteriophages...co-founders raised $6 million. The biggest challenge during this time was learning to communicate effectively...

  3. Human Germline Editing Using CRISPR

    Type
    Blog Post
    ... really, and what new questions does this paper bring up? We’ll sift through the paper to understand what...results in fewer eggs. However, the potentially staggering costs of such a treatment on top of the expense... Mary Gearing...

  4. Summer SciComm Series: Cognitive Load

    Type
    Blog Post
    ...placing on your audience when you're in the midst of sharing your science - which can drive away even the most... them restate or summarize the concepts you’re covering. Don't show frustration if they're incorrect; ...as people discuss their experiences. If you’re hearing multiple people with similar misconceptions, it...

  5. Antibodies 101: Polyclonal Antibodies

    Type
    Blog Post
    ...polyclonal antibodies include any antibodies produced during the immune response.   Polyclonal antibodies...If your protein might become slightly denatured during your experimental protocol, using a polyclonal ... (2019) Antibody Purification and Storage. Cold Spring Harb Protoc 2019:pdb.top099101. https://doi.org...

  6. Plasmids 101: Golden Gate Cloning

    Type
    Blog Post
    ...within both the synthetic biology and genome engineering fields. We’ll walk you through how to apply this... Engler & Marillonet. Golden Gate and genome engineering In early 2011, the Bogdanove and Voytas groups... Mary Gearing...

  7. Working with Nuclear Receptors

    Type
    Blog Post
    ...mining tool, Transcriptomine. We are currently nearing the completion of an 18-month effort to overhaul...identifying plasmids in the research literature and acquiring them in a timely manner. Addgene represents an...an excellent solution to the problem of sharing plasmids, providing researchers with a one-stop shop for...

  9. Early Career Researcher Toolbox: Finding Relevant Papers

    Type
    Blog Post
    ...publications in the fields of research they support. Monitoring these types of accounts can help you find papers...papers later. Pro tip Learn more about authors by exploring author pages (for example, Melina Fan’s author... Instead, you create your own research feed by entering keywords, concepts, journals, and/or papers of...

  11. Science communication: 9 Strategies to Get Your Foot in the Door

    Type
    Blog Post
    ...create a successful science blog  5) Get creative – Bring your knacks into play Perhaps writing just isn’t...projects, you can set up a citizen science project. Answering the big science questions around climate change...guest blogger, Sarah Schmidt! Sarah Schmidt is engineering resistance against Fusarium wilt disease in bananas...

  12. Engaging with science and society at pgEd

    Type
    Blog Post
    ...disparities. But as the science moves forward at a blistering pace, it is becoming ever more urgent for scientists...ways of collaborating, and to organize events that bring different voices together to talk about genetics...running these events will be important both in preparing you for future work in these areas, and in helping...

  13. Educational Planarian Tools for Teaching Developmental Biology Techniques

    Type
    Blog Post
    ...characterized in an unrelated worm (C. elegans) [2]. Delivering dsRNA matching a specific mRNA sequence triggers...and silences its expression. More specifically, during the process of RNAi, an enzyme dubbed Dicer cuts...smaller dsRNA fragments are referred to as small interfering RNAs (siRNA) and they bind to a class of Argonaute...

  14. Lab Automation at Addgene

    Type
    Blog Post
    ...out your samples. This can be useful if you’re transferring samples from 96 wells plates onto agar q-trays...bacterial samples at the same time as opposed to preparing each sample individually. Enter the BIOROBOT! ...brushes the information onto the vials instead of requiring us to put a label on each tube by hand. We’ll ...

  15. Split Fluorescent Proteins for Studying Protein-Protein Interactions

    Type
    Blog Post
    ...produced when the candidate proteins’ interaction brings the pieces of the FP back together (Figure 1). ...interaction in vivo or in vitro, and can be used for measuring protein expression or localization in cells, or...yeast TREX-2 mRNA export complex: components and anchoring nucleoporin. Lu et al. Plant J. 2010 Yuhai Cui...

  16. Simple CRISPR-based Epigenetic Editing: dCas9-directed DNA Demethylation

    Type
    Blog Post
    ...University. Some enzymatic fusions are godsends. Tethering dCas9 to the histone acetyltransferase p300 or... 2018). This means that off-target loci – in neighboring chromatin or throughout the genome – could be...that site. So, when DNMT1 methylates the genome during DNA replication, it doesn’t methylate sites blocked...

  17. Design Tips for Prime Editing

    Type
    Blog Post
    ...extension begins with a C base can cause unwanted base pairing (left), while pegRNAs that use other bases in this...the cell to use the edited strand as a template during DNA repair. Test multiple nick sites, starting ... linkers that minimize unwanted intra-RNA base pairing with the primer binding site (Nelson et al., 2022...

  18. Hot Plasmids February 2024

    Type
    Blog Post
    ...here!   Zhang, Z., et al. (2023). Efficient engineering of human and mouse primary cells using peptide-assisted...effective at breaching the blood-brain barrier and delivering a transgene, with neuron bias observed in young...the Gradinaru lab, AAV1-X1, was developed by transferring the 7-mer peptide region from variable region...

  19. 6 Tips for Grant Writing

    Type
    Blog Post
    ...approach that problem in a novel way. Finally, engineering new applications to solve problems will provide...’s a good idea to move around to different labs during your career; you learn new techniques and interact...get feedback from the funding body through their scoring system or meeting with their grant submission panel...

  20. How-to: 5 Steps to a Great Panel Discussion

    Type
    Blog Post
    ...the moderator should be taking good notes and discovering interesting stories, riffs or opinions that each... a fun discussion. People are much better at remembering these things on a relaxed phone call than when...The idea is to ask questions in a directed way to bring out these interesting stories and remind the panelists...
Showing: 601 - 620 of 1006 results