We narrowed to 1,006 results for: RING

...polyclonal antibodies include any antibodies produced during the immune response.   Polyclonal antibodies...If your protein might become slightly denatured during your experimental protocol, using a polyclonal ... (2019) Antibody Purification and Storage. Cold Spring Harb Protoc 2019:pdb.top099101. https://doi.org...

...within both the synthetic biology and genome engineering fields. We’ll walk you through how to apply this... Engler & Marillonet. Golden Gate and genome engineering In early 2011, the Bogdanove and Voytas groups... Mary Gearing...

...mining tool, Transcriptomine. We are currently nearing the completion of an 18-month effort to overhaul...identifying plasmids in the research literature and acquiring them in a timely manner. Addgene represents an...an excellent solution to the problem of sharing plasmids, providing researchers with a one-stop shop for...

...disparities. But as the science moves forward at a blistering pace, it is becoming ever more urgent for scientists...ways of collaborating, and to organize events that bring different voices together to talk about genetics...running these events will be important both in preparing you for future work in these areas, and in helping...

...characterized in an unrelated worm (C. elegans) [2]. Delivering dsRNA matching a specific mRNA sequence triggers...and silences its expression. More specifically, during the process of RNAi, an enzyme dubbed Dicer cuts...smaller dsRNA fragments are referred to as small interfering RNAs (siRNA) and they bind to a class of Argonaute...

...out your samples. This can be useful if you’re transferring samples from 96 wells plates onto agar q-trays...bacterial samples at the same time as opposed to preparing each sample individually. Enter the BIOROBOT! ...brushes the information onto the vials instead of requiring us to put a label on each tube by hand. We’ll ...

...simplicity of gene activation: rather than requiring protein engineering for each loci, CRISPR/Cas systems only...regulation. This can be used to activate transcription during either initiation or elongation, depending on which...

...faster in the following test and skew results measuring locomotion. Mice may need to be given some time...optional handling animals Experiment Behavioral scoring systems (experimenter differences in behavior observation...of our viral vectors blog posts Find tips for preparing AAVs View our Beginner's Guide to Viral Vectors...

...create a successful science blog  5) Get creative – Bring your knacks into play Perhaps writing just isn’t...projects, you can set up a citizen science project. Answering the big science questions around climate change...guest blogger, Sarah Schmidt! Sarah Schmidt is engineering resistance against Fusarium wilt disease in bananas...

...produced when the candidate proteins’ interaction brings the pieces of the FP back together (Figure 1). ...interaction in vivo or in vitro, and can be used for measuring protein expression or localization in cells, or...yeast TREX-2 mRNA export complex: components and anchoring nucleoporin. Lu et al. Plant J. 2010 Yuhai Cui...

...University. Some enzymatic fusions are godsends. Tethering dCas9 to the histone acetyltransferase p300 or... 2018). This means that off-target loci – in neighboring chromatin or throughout the genome – could be...that site. So, when DNMT1 methylates the genome during DNA replication, it doesn’t methylate sites blocked...

...extension begins with a C base can cause unwanted base pairing (left), while pegRNAs that use other bases in this...the cell to use the edited strand as a template during DNA repair. Test multiple nick sites, starting ... linkers that minimize unwanted intra-RNA base pairing with the primer binding site (Nelson et al., 2022...

...here!   Zhang, Z., et al. (2023). Efficient engineering of human and mouse primary cells using peptide-assisted...effective at breaching the blood-brain barrier and delivering a transgene, with neuron bias observed in young...the Gradinaru lab, AAV1-X1, was developed by transferring the 7-mer peptide region from variable region...

...publications in the fields of research they support. Monitoring these types of accounts can help you find papers...papers later. Pro tip Learn more about authors by exploring author pages (for example, Melina Fan’s author... Instead, you create your own research feed by entering keywords, concepts, journals, and/or papers of...

...approach that problem in a novel way. Finally, engineering new applications to solve problems will provide...’s a good idea to move around to different labs during your career; you learn new techniques and interact...get feedback from the funding body through their scoring system or meeting with their grant submission panel...

...the moderator should be taking good notes and discovering interesting stories, riffs or opinions that each... a fun discussion. People are much better at remembering these things on a relaxed phone call than when...The idea is to ask questions in a directed way to bring out these interesting stories and remind the panelists...

... unable to participate in genetic recombination during meiosis. Also, flies are not viable if they are...know is that males do not recombine chromosomes during meiosis. This can sometimes be a handy feature ...Like Addgene, the Drosophila community is highly sharing-oriented, and there are many resources to help ...

...separate proteins should be useful for monitoring cellular events occurring at varied time scales.  Table 1:...the FP timers, add an entire new dimension to monitoring localization; using FP timers, researchers can...

...model Arabidopsis (Weiss et al., 2025). By co-delivering TRV1 with TRV2 that encodes ISYmu1 and an ωRNA...hematopoietic stem cells and track clonal dynamics during differentiation after they introduced mutations...tissue tropism for gene therapy and ex vivo cell engineering applications and is especially well-suited for...

...more gRNAs must be used along the same chromosome. During DNA repair, there is a chance that the two breakpoints...Harnessing type I CRISPR–Cas systems for genome engineering in human cells. Nature Biotechnology, 37(12),...CRISPR-Cas Systems INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons Additional resources...