We narrowed to 928 results for: TIM;
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TypeBlog Post... or yellow fluorescent protein at the time. It is 1.5 to 3 times brighter than the most commonly used ...
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Why Add Sucrose? Improved Yields for Adeno-associated Virus Preparation
TypeBlog Post... be spending less time and resources resupplying our current inventory and more time providing new materials... -
Plasmids 101: Origin of Replication
TypeBlog Post...you have only one plasmid type in your cells at a time? Do you want to make a lot of your plasmid? Is the...streaked bacteria have higher copy numbers – for optimal results always pick a single colony and do not ... -
Hot Plasmids - June 2020 - Barcoded CRISPR Library, Sparse Cell Labeling, Calcineurin Reporter, and DNA Staining Dye Alternative
TypeBlog Post...tracing of the performance of each gRNA multiple times within the same experiment by counting both the ...restriction enzyme digestion of single molecules in real-time. Find plasmids expressing tTALE-FP Shin et al., ... -
Summer SciComm: Science Storytelling
TypeBlog Post... experiment has not worked…again…for the seventh time…. I find that once I sit down and talk to a scientist...the midst of all these storytelling devices, we sometimes forget the most important thing about science:... -
Addgene Begins Distribution of Recombinant Antibodies
TypeBlog Post...neuroscience research and other disciplines, over time. The resource may also expand to include other affinity...collection to become more useful to everyone over time. Through our incredible community, including your... -
Three Tips for Preventing Viral Plasmid Recombination in Your Samples
TypeBlog Post... replicate, and the transgene will be lost. Over time, bacteria containing the recombined vector backbone...post. Check yourself before you recA yourself. Optimize Growth Conditions To reduce recombination, it ... -
What the HEK?
TypeBlog Post...community, many cellular research tools have been optimized specifically for and in HEKs. These systems built...are immortalized, but cell health can degrade over time in culture. This can affect factors that could skew... -
Plasmid Cloning by PCR
TypeBlog Post... making a copy of a piece of DNA and at the same time adding restriction sites to the ends of that piece...both function in the same buffer, as this will save time later In our example, we will use EcoRI and NotI... -
Plasmids 101: A Brief History of Plasmids and an Improved eBook!
TypeBlog Post... that could be transferred between cells. At the time, these extranuclear agents of heredity were thought...the widely adopted name for these elements. At the time, the use of episome seemed appropriate, especially... -
Donations from Addgene to Yield Answers for Rare Disease Researchers
TypeBlog Post...described in 1971,” McSweeney said. “But, it is still sometimes wrongly diagnosed as a seizure disorder.” In 2012... working on several genetic diseases at the same time. The limitation of budget restrained us from costly... -
Interview: Ed Boyden on Optogenetics, Neuroscience, and the Future of Neuroengineering
TypeBlog Post...compete against each other. Getting two-color stimulation has been a holy grail of the field. The problem...to try these optogenetic tools out for the first time? Boyden: There are always considerations for each... -
"Build Your Plasmid": The Game - Play to Teach
TypeBlog Post...playing with some cards and I had a quick flashback to time spent playing a card game called “Magic”. There ...certain target cell population. Who is the winner? Ultimately, society should be! This is a collaborative game... -
New and Upcoming Viral Vectors - September 2019
TypeBlog Post... four variants of these biosensors, which are optimized for specific parameters: jGCaMP7s is the most...specificity of influence measurements by reducing photostimulation of the axons and dendrites of non-targeted... -
Addgene-Seeding Labs plasmid grant round 3: microbial drug resistance and biofilms, antidiabetic plant components, visualizing brain injury - and a glowing robot fish
TypeBlog Post...understanding of PZA action and could potentially stimulate the development of useful diagnostic methods and...non-linear microscopy methods will be used to stimulate and manipulate neural populations in order to ... -
Unique and persistent IDs for improved reproducibility: Addgene now supports RRIDs and compact identifiers for all plasmids
TypeBlog Post...reproducibility because mistakes in this can lead to wasted time or retractions. Using a catalog number for identifying...are verified and identifiable to save researchers time and make their work more reproducible. While Addgene... -
Summer SciComm Series: A PhD in Science Communication
TypeBlog Post...body and how it works. I used to stay up past my bedtime, poring over my grandparents’ medical textbooks...communication as a PhD student Fortunately, by the time I was ready to graduate with my BSc in Anatomy, ... -
Crowdfight, a Platform to Boost Scientific Collaboration During COVID-19 and Beyond
TypeBlog Post...scientists of all areas who volunteered to donate their time and expertise to help them. In less than two weeks...and easily credited. And nothing stops these one-time favors from becoming starting points for long-lasting... -
Hot Plasmids - December 2019 - CRISPR pooled library, LipoGlo, CRISPRa, and Voltage Imaging Tools
TypeBlog Post...voltage indicators (GEVIs) change fluorescence in real time when neurons or muscle cells transmit electrical... and conditions that can be used to monitor real time voltage changes in worms and have made these constructs... -
Communicating Your Science With Help From ComSciCon
TypeBlog Post... and develop skill in science communication over time. Like most skills, great science communication ... to pose these why questions to yourself, but ultimately there is no substitute for feedback from others...