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We narrowed to 1,076 results for: TOR

Showing: 601 - 620 of 1076 results

  1. No Llamas Required - Synthetic Nanobodies Against Membrane Proteins

    Type
    Blog Post
    ...subcloned (using FX cloning) into the expression vector pSb-Init, which attaches a Myc-tag and a His6-tag... and isolate sybodies targeting the SARS-CoV-2 Receptor Binding Domain found on the membrane-bound spike... Synthetic nanobodies targeting the SARS-CoV-2 receptor-binding domain. BioRxiv.  https://doi.org/10.1101....16.045419.  Wrapp D, De Vlieger D, Corbett KS, Torres GM, Wang N, Van Breedam W, Roose K, van Schie L...

  2. Quick Guide to Working with Drosophila Part 1: Getting Started with Flies

    Type
    Blog Post
    ...daytime. They mostly stop emerging at night. An incubator with a light cycle makes for very predictable ... phenotypes, check out the Classical Genetics Simulator website. One interesting thing to know is that...research moving. Below, you’ll find some great introductory resources. As you dive deeper into Drosophila... the upper right hand corner. They have video tutorials under the help tab like this one to get you started...

  3. Plasmids 101: Choosing an Antibiotic Resistance Gene

    Type
    Blog Post
    ...Ampicillin Ampicillin, often just called amp, is the historical king of the antibiotic resistance realm. Amp ...thankfully, most competent cells do not express this factor. Zeocin is handy because it can be a one-stop shop...Hadley, W. K., & Brooks, G. F. (1986). Effect of storage temperature and pH on the stability of antimicrobial...bonded proteins in its cytoplasm. Microbial Cell Factories, 11, 56. https://doi.org/10.1186/1475-2859-11-...

  4. Technical Design of a Western Blot

    Type
    Blog Post
    ...like rotting eggs but has a longer shelf life when stored properly. Because BME is not as strong as DTT, ..., while expensive, reduce protocol time, can be stored at 4 °C, and are very consistent gel-to-gel.  Ladder...multiple times. It doesn’t degrade during long-term storage and has both high binding capacity and high protein...inexpensive and easily made in the lab. They can be stored at your bench during the procedure and at 4 °C ...Fluorescent proteins are stable, so blots can be stored for weeks to months without loss of signal. While...

  5. New CRISPR Tools: Cas7-11 and PASTE

    Type
    Blog Post
    ... weird black pellet's DNA yielded a new CRISPR effector protein they named Cas7-11. Testing showed that.... Eugene Koonin and Kira Makarova, longtime collaborators of Omar and Jonathan and experts in CRISPR evolution...single protein, shedding the complexity of its ancestors. They classified the system as Class I, Type III-E...Programmable RNA targeting with the single-protein CRISPR effector Cas7-11. Nature. 2021 Sep;597(7878):720-725. doi...

  6. Plasmids 101: Stringent Regulation of Replication

    Type
    Blog Post
    ...carefully regulated by a mix of positive and negative regulators. The genome of E. coli is about 4.6 million ...bacteria, however, the protein integration host factor (IHF) assists with the process: it bends the DNA...trading out ADP for ATP to re-activate DnaA. These regulatory mechanisms allow E. coli to control chromosomal...nar/gky457 Park, K., Han, E., Paulsson, J., & Chattoraj, D. K. (2001). Origin pairing (‘handcuffing’) ...

  7. Antibiotic Resistance: An Old Solution but a New Problem

    Type
    Blog Post
    ...possess resistance genes survive and multiply. Factors contributing to the development of resistance include...www.youtube.com/watch?v=sx1uDYSfINA. When you go to the doctor, it will very likely take time to determine if ...resistant infection or not, because scientists in the laboratory will need to isolate and grow whatever bacteria...method that is frequently used in the clinical laboratory. What can we do to stop the emergence of antibiotic...

  8. Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection

    Type
    Blog Post
    ... green fluorescent protein (GFP) from Aequorea victoria and its variants are genetically encoded fluorescent... of fluorescent proteins derived from Aequorea victoria GFP sticks out and can be considered a linker ...Janetopoulos, Chris, Tian Jin, and Peter Devreotes. "Receptor-mediated activation of heterotrimeric G-proteins...et al. "Expanded dynamic range of fluorescent indicators for Ca2+ by circularly permuted yellow fluorescent...

  9. Deep Mutational Scanning with One Pot Saturation Mutagenesis

    Type
    Blog Post
    ...site of interest can be cloned into an expression vector with Gibson assembly. While this is a comprehensive...is generated by transforming a backbone phagemid vector into an E. coli strain that is deficient in dUTPase... combine pairs of mutations together to test combinatory effects. One-pot saturation mutagenesis The ...2 instances where it does. 1) When the backbone vector has multiple BbvCI sites. If these sites have opposite...

  10. Antibodies 101: Normalization and Loading Controls for Western Blots

    Type
    Blog Post
    ...Ah, the notorious western blot: we meet again. So useful, yet so finicky to design and optimize. Today...due to loading and transfer errors — not to any factors from before the proteins even hit the gel. But ...all, while overloaded proteins may saturate the detector. In between these extremes lies the linear detection...concentration as well as by antibody dilution — both factors we’ve covered in our previous blog posts (1, 2)...

  11. Proximity Labeling: A Powerful Tool for Protein Complex Purification and Proteomic Mapping

    Type
    Blog Post
    ...Split-BioID: A conditional proteomics approach to monitor protein-protein interactions Split-BioID combines... a split BirA* molecule that can reassemble to restore activity when fused to two interacting proteins... in one experiment a scientist can confirm and monitor the interaction of two proteins while also biotin...Split-BioID a conditional proteomics approach to monitor the composition of spatiotemporally defined protein...

  12. Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

    Type
    Blog Post
    ...have previously used histone deacetylase (HDAC) inhibitors to increase transcription of the integrated HIV...turned to the CRISPR/Cas9 Synergistic Activation Mediators (SAM) system available from Addgene. This system...TALENs focused primarily on disrupting CCR5, the receptor that HIV-1 uses to enter a cell. ZFNs and CRISPR...W. CRISPR/gRNA-directed synergistic activation mediator (SAM) induces specific, persistent and robust ...

  13. Troubleshooting and Optimizing a Western Blot

    Type
    Blog Post
    ...reduced by adding a protease inhibitor cocktail and phosphatase inhibitors to your lysis buffer. Additionally...ladder runs off the gel. Luckily, this is easily monitored in real time, as the dye front (and many ladders...resources with examples, like BioRad’s Western Blot Doctor page, or robust discussions, like ResearchGate’...longer time Protein degradation Add protease inhibitors to lysate Insufficient denaturing conditions...

  14. Antibodies 101: Validation

    Type
    Blog Post
    ...to sort cells based on the expression of a new receptor that is of interest to you. You found a couple...may not be the most accessible option for many laboratories. Additionally, it can be difficult to distinguish...understand if protein:protein interactions could be a factor in your analysis. Example: You are planning an ...Koch-Nolte F, Pascual N, Rodríguez-Barbosa J-I, Torensma R, Turley H, Pulford K, Banham AH (2015) The European...

  15. Welcome to the Lab Bench! New Protocol Series Helps Trainees Get Started in the Lab

    Type
    Blog Post
    ...protocols for plasmid cloning and working with viral vectors, but what we lacked were videos guiding completely...completely new members of the lab or those taking introductory lab courses. The Intro to the Lab Bench series... need a refresher on some best practices for introductory science techniques. It’s also great for teachers...

  16. Antibodies 101: Introduction to Antibodies

    Type
    Blog Post
    ...improve its stability. Additionally, the choice of vector can impact the expression level of an antibody ...lesional skin from a psoriasis patient, showing an inflammatory phenotype. Each protein was detected using an... cellular pathways by binding to cell surface receptors. These applications are typically used for investigating...Arbabi-Ghahroudi M (2017) Camelid Single-Domain Antibodies: Historical Perspective and Future Outlook. Front Immunol...

  17. Trends in CRISPR and SynBio Technologies

    Type
    Blog Post
    ...fields.  Addgene is a global nonprofit plasmid repository. Over 2,000 labs have deposited plasmids to Addgene...Zhang acknowledge the role of data and materials repositories in aiding the dramatic spread of the Cas9 technology...accelerated through a combination of open-source distributors such as Addgene, as well as a number of online...

  19. Selecting Your Plasmid Purification Kit

    Type
    Blog Post
    ...any applications. DNA quantity is fairly self explanatory and achieved by performing different sized preps...about!) are a significant source of waste in laboratories. To help combat this, some manufacturers have...Minimized amount of silica ACT environmental impact factor label Non-silica based, avoids use of chaotropic...kit for your lab is dependent on many different factors as we detailed above, so be sure to pick what kit...

  20. Top Requested Plasmid of 2017 - pMD2.G

    Type
    Blog Post
    ...2016 were CRISPR plasmids), but so are lentiviral vectors, many of which are used to deliver Cas9 and other....G from Didier Trono’s lab! Lentivirus envelope vector pMD2.G This plasmid has been requested nearly 6,000... our Viral Production Process Find other Viral Vectors ...
Showing: 601 - 620 of 1076 results