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We narrowed to 888 results for: TRI

Showing: 601 - 620 of 888 results

  1. Finding Your Science Policy Path

    Type
    Blog Post
    ...This post was contributed by Steph Guerra, a AAAS Science and Technology Policy Fellow at the Veterans... challenging if you are trying to apply for an outright job in a field where you have no specific scientific...

  2. Tips for a 1st Time CRISPR User (by a 1st Time CRISPR User)

    Type
    Blog Post
    ...all know that in the lab there are often little tricks that are essential for experiments but that nobody... not (Figure 4). 5. In theory you could use densitometric analysis to quantitate the intensity of the ...

  3. Antibodies 101: Designing Your First Flow Panel

    Type
    Blog Post
    ...your cells. While proteins themselves can emit intrinsic fluorescence when excited with ultraviolet (UV...use a dimmer fluorophore. However, these aren't strict rules and in practice the differences in brightness...

  6. A Needle in a Base-Stack: Cas9 Structural Biology

    Type
    Blog Post
    ... nuclease function. Stem loops 2 and 3 are not strictly necessary for in vitro cleavage, but they improve...Figure 3 crystal structure, reflecting the less stringent recognition mechanism that can tolerate mismatches...

  7. Antibody Validation for Flow Cytometry

    Type
    Blog Post
    ...different fix/perms (methanol, PFA-saponin, PFA-triton). The fix/perm solutions can make a significant...compared, 4% PFA and 0.1% saponin; 4% PFA and 0.1% Triton X-100; and methanol, we found that the optimal ...

  8. Delivery Methods for Generating iPSCs

    Type
    Blog Post
    ...chimeric offspring shows the ability of the iPSC to contribute to all three germ layers, and the ability of ...iPSCs to form functional germ cells. The highest stringency test for mouse iPSCs, tetraploid complementation...

  11. 'Tis the Season to #DeckTheLab

    Type
    Blog Post
    ...bar is set very high from last year’s impressive entries, but we have faith our community of creative scientists...

  12. Addgene's 20th Anniversary Party

    Type
    Blog Post
    ...main purpose of the party was to celebrate the contributions of each and every Addgenie, from our co-ops ...

  13. It’s CRISPR Week at Addgene!

    Type
    Blog Post
    ...have been shared over 140,000 times to over 75 countries. That’s a lot of CRISPR! As we’ve shared these...

  14. We’re Going Viral This Week!

    Type
    Blog Post
    ...platform for scientists using AAV to find and contribute experimental information, such as titer, serotype...

  17. The Twelve Days of CRISPR

    Type
    Blog Post
    ...third day of CRISPR, Addgene gave to me: tips and tricks for designing gRNAs for CRISPR editing. On the ...
Showing: 601 - 620 of 888 results