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We narrowed to 749 results for: UTY

Showing: 601 - 620 of 749 results

  2. Clear and Accessible Writing

    Type
    Blog Post
    ...macromolecule density is equal to the buoyant force of the solution. Contaminants of lower densities are captured ...

  10. Antibody Validation Using the Indirect ELISA Method

    Type
    Protocol
    ...different incubation options available. Caution Use the Stop Solution in a well ventilated area and avoid ...Immediately before use, mix 5 mL TMB Solution and 5 mL Peroxide Solution from the TMB substrate kit. Cap the...determine the ideal concentration. Prepare serial dilutions of the purified antigen as follows: 2 ng/µL : ... wells of each row, using a different standard solution for each row of a 96-well ELISA microplate. Cover...the wash buffer from the wells. Prepare antibody dilution buffer (0.1% BSA in PBS) as follows: Dilute 50... to the desired concentrations in the antibody dilution buffer. Pro-Tip The ideal antibody concentration...multichannel pipette, add 100 µL of primary antibody solution to the appropriate wells. Cover the plate with...

  11. Kit Free RNA Extraction

    Type
    Protocol
    ...Option #1 - Solution D Protocol Before starting this protocol, make sure to prepare solution D (see reagent...or lyse tissues or cells in Solution D. For tissues: use 1 mL of Solution D per 100 mg of cells. For cultured...resuspension. This protocol provides two options: using Solution D (see reagent section for recipe) or using an...all-in-one acid guanidinium thiocyanate-phenol solution such as TRIzol®. For the precipitation step, two...reagents RNase-free (Use an RNase decontamination solution, such as RNaseZap® or RNase AWAY®, may be used... Vortexer -20°C freezer -80°C freezer Reagents Solution D (for Protocol Option #1 ): 4 M Guanidinium Thiocyanate...alcohol (49:1) 75% Ethanol RNase-free water or TE solution RNase free tubes: microcentrifuge tubes, 4 mL ...
Showing: 601 - 620 of 749 results