...activated upon binding of CNO thus showing no constitutive activity. There are an array of DREADDs that...

...control signaling provides greater spatiotemporal resolution than pharmacological or genetic manipulation ...

...the fluorescent protein properties need to be scrutinized under relevant conditions. The properties of ...

...luminescence to get around these issues, but this solution wasn’t practical due to the low intensity of luminescent...

...recombineering to create insertions, deletions, and substitutions - at the same efficiency as Gibson Assembly ...

...less than an hour, thus a population of cells constitutively expressing a targeted Cas9 should possess indels...

... use in various cell types and in live mice. Therapeutic uses in humans are also on the table. “In the...

...Goal setting is easier with more perspectives contributing to the process Senior people (of which there...

...their advice and time. Consider this option but be cautious and make sure to choose one that comes highly ...

...chance they will bind that sequence. That’s the revolutionary aspect: TALENs take a technology that was difficult...

... Mix the dilutions well Note: the 1:10 dilution can usually be omitted because this dilution typically... serial dilutions of the lentivirus into DMEM complete containing 10 μg/mL polybrene: Dilution Volume ...quantified. Add 150 μL of a viral dilution to each well (each well gets one dilution) Also include an untransduced... colonies for at least 2 of the dilutions. Pro-Tip The higher dilution wells tend to provide more accurate...present in the lower dilutions, and single colonies are visible in the higher dilutions. Last reviewed on...staining resistant colonies with crystal violet solution and, therefore, the cells cannot be used for later...DMEM complete containing 10 μg/mL polybrene by diluting 20 μL of 10 mg/mL polybrene into 20 mL of media...

...fluorescent protein (i.e. GFP, RFP) Detecting GFP reconstitution across synaptic partners (GRASP) assays No...

...unexpected: termites. “I was really interested in the evolution of social behaviour in social insects, obviously...

...information available. When I am speaking at a new institution, for example, I will often see a whole bunch ...

...fluorescent markers to visualize localization and distribution of gut bacteria without fixation or preparation...

...are more evenly controlled. The Grefen Lab has utilized the 2in1 cloning system to generate a series of...

...microenvironments shape a tumor’s evasiveness, evolution, and response to treatment. Some microenvironment...

...applications, smaller affinity reagents means higher resolution. Many of the below are also small enough to be...

...directly-conjugated antibody will provide better resolution as these immune complexes are smaller than a ...

...the ssAAV genome has a mutation in the terminal resolution site (TRS) to form a vector genome with wild-...