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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 746 results for: UTY

Showing: 601 - 620 of 746 results

  9. Kit Free RNA Extraction

    Type
    Protocol
    ...Option #1 - Solution D Protocol Before starting this protocol, make sure to prepare solution D (see reagent...or lyse tissues or cells in Solution D. For tissues: use 1 mL of Solution D per 100 mg of cells. For cultured...resuspension. This protocol provides two options: using Solution D (see reagent section for recipe) or using an...all-in-one acid guanidinium thiocyanate-phenol solution such as TRIzol®. For the precipitation step, two...reagents RNase-free (Use an RNase decontamination solution, such as RNaseZap® or RNase AWAY®, may be used... Vortexer -20°C freezer -80°C freezer Reagents Solution D (for Protocol Option #1 ): 4 M Guanidinium Thiocyanate...alcohol (49:1) 75% Ethanol RNase-free water or TE solution RNase free tubes: microcentrifuge tubes, 4 mL ...

  10. Virus Protocol - Generating Stable Cell Lines

    Type
    Protocol
    ...viral dilution to each well (each well gets one dilution, so a 6-well plate will hold 5 dilutions plus ...just a sample of possible dilutions. You may want to try higher/lower dilutions depending on your downstream...the results of your titration. Mix the dilutions well. Dilution Volume of Lentivirus (μL) Volume of DMEM...that already contain 0.5 mL of virus solutions at various dilution. Make sure to use the polybrene-containing...batch of DMEM complete + 10 µg/mL polybrene by diluting 20 µL of 10 mg/mL polybrene into 20 mL media. ...aliquot on ice prior to use. Prepare a range of dilutions of the lentivirus in DMEM complete + 10 µg/mL ...polybrene-containing media to make the cell solution in this step. To seed the cells: Prepare a batch of cells as ...

  11. Protocol - How to Run an Agarose Gel

    Type
    Protocol
    ...swirling the flask occasionally as the solution heats up.). Caution HOT! Be careful stirring, eruptive boiling...do you get better resolution of bands? A few simple ways to increase the resolution (crispness) of your...gel electrophoresis, including tips to improve resolution and separation of bands. Protocols... completely dissolved (but do not overboil the solution, as some of the buffer will evaporate and thus...continue towards a boil. Keep an eye on it the solution has a tendancy to boil over. Placing saran wrap...necessary if you pay close attention. Let agarose solution cool down to about 50 °C (about when you can comfortably...0.2-0.5 μg/mL (usually about 2-3 μl of lab stock solution per 100 mL gel). EtBr binds to the DNA and allows...

  18. Antibodies 101: Affinity Reagents

    Type
    Blog Post
    ...applications, smaller affinity reagents means higher resolution. Many of the below are also small enough to be...
Showing: 601 - 620 of 746 results