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Showing: 601 - 620 of 841 results
  1. New Videos: Addgene Lab Tips

    Type
    Blog Post
    ...hopefully make your life a little easier. These can range from technical tips as in our episode on Barcoding...
  2. Affinity Purification of Recombinant Antibodies with Protein A or Protein G

    Type
    Protocol
    ...Option 2 with a buffer exchange/concentration using an Amicon 30 kDa MWCO buffer exchange column. (Optional... Day 1: Purify antibody Day 2 or later: Buffer exchange Equipment Class II, Type A2 Biological Safety ...above 1.0 mg/mL proceed to Option 1 with a buffer exchange using a Zeba Spin 7 kDa MWCO desalting column....the same recombinant antibody. Section 2: Buffer exchange Choose Option 1 or Option 2 based on the concentration...concentration of the pooled sample above. Option 1: Buffer exchange using a desalting column The 10 mL Zeba Spin Desalting...sterile sodium azide to 1 mM. Option 2: Buffer exchange and concentration with an Amicon column Apply ...least 4 additional times to ensure a full buffer exchange. Discard the flow through into a waste container...
  3. Our New Antibody Mascot is....

    Type
    Blog Post
    ...out to you and received a plethora of options, ranging from Affie to Yncredibody. After an agonizing week...
  4. Countdown to Halloween @Addgene

    Type
    Blog Post
    ...guess each plasmid? Our lab paid tribute to the changing seasons – and they also invited Father Time to...
  5. New Viral Vectors - Spring 2025

    Type
    Blog Post
    ...diverse needs of our community and support a wide range of experimental designs. Here are nine great additions...
Showing: 601 - 620 of 841 results