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TypeBlog Post...Hot Plasmid 3: CRISPR base editors with enhanced range and specificity. Listen to to the Segmennt of Improved...
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Addgene is Stopping Use of X (formerly Twitter)
TypeBlog Post...gradual move off X as a response to major platform changes, such as limiting access and increased priority... -
Trends in CRISPR and SynBio Technologies
TypeBlog Post... the authors Patrick Hsu, Eric Lander, and Feng Zhang acknowledge the role of data and materials repositories... -
New FLIS Plasmid (April Fools 2022)
TypeBlog Post...Phuels lab deposited a plasmid that will forever change the fly lab experience. The Frequency Light Interval... -
Participate in the Pets of Addgene Scavenger Hunt
TypeBlog Post... Jennifer Tsang... -
We’re Going Viral This Week!
TypeBlog Post... Jennifer Tsang... -
Congrats to the 2019 Deck The Lab Winners
TypeBlog Post... Jennifer Tsang... -
Export Control Regulations Lifted for Plasmids Containing Rabies and VSV-G Genes
TypeBlog Post...export controls and regulations are complex and may change suddenly. Please email [email protected] for the... -
New Videos: Addgene Lab Tips
TypeBlog Post...hopefully make your life a little easier. These can range from technical tips as in our episode on Barcoding... -
Expanded Access to Rabies Plasmids
TypeBlog Post...our normal process. Due to recent regulation changes, an export control license for rabies materials... -
Affinity Purification of Recombinant Antibodies with Protein A or Protein G
TypeProtocol...Option 2 with a buffer exchange/concentration using an Amicon 30 kDa MWCO buffer exchange column. (Optional... Day 1: Purify antibody Day 2 or later: Buffer exchange Equipment Class II, Type A2 Biological Safety ...above 1.0 mg/mL proceed to Option 1 with a buffer exchange using a Zeba Spin 7 kDa MWCO desalting column....the same recombinant antibody. Section 2: Buffer exchange Choose Option 1 or Option 2 based on the concentration...concentration of the pooled sample above. Option 1: Buffer exchange using a desalting column The 10 mL Zeba Spin Desalting...sterile sodium azide to 1 mM. Option 2: Buffer exchange and concentration with an Amicon column Apply ...least 4 additional times to ensure a full buffer exchange. Discard the flow through into a waste container... -
Addgene’s New Way to Help You Share Science
TypeBlog Post...they’ve used at their lab benches. It’s these exchanges with leading scientists (and our growing collection... -
Depositor Week 2020 is Happening Now. Join us!
TypeBlog Post... Jennifer Tsang... -
Our New Antibody Mascot is....
TypeBlog Post...out to you and received a plethora of options, ranging from Affie to Yncredibody. After an agonizing week... -
#DeckTheLab with Tinsel and Test Tubes, Fa La La La La La La La La
TypeBlog Post...might decorate your lab in pipette tip snowflakes, hang stockings filled with tube rack “gifts”, or prepare... -
Streaking for Single Colonies: The Streak Plate Challenge
TypeBlog Post... Jennifer Tsang... -
Countdown to Halloween @Addgene
TypeBlog Post...guess each plasmid? Our lab paid tribute to the changing seasons – and they also invited Father Time to... -
Interview with Bart Newland, Addgene’s Newest Board Member
TypeBlog Post... Jennifer Tsang... -
New Viral Vectors - Spring 2025
TypeBlog Post...diverse needs of our community and support a wide range of experimental designs. Here are nine great additions... -
Celebrating One Million Plasmids Shared!
TypeBlog Post... Jennifer Tsang...