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We narrowed to 951 results for: cha

Showing: 601 - 620 of 951 results

  1. Of Myc and Men

    Type
    Blog Post
    ...protein tagging. In two studies investigating mechanisms of endoplasmic reticulum localization, Munro ...also find anti-Myc [9E10] Fab fragments and single-chain variable fragments.  Abi, on the other hand...glucose-regulated protein and immunoglobulin heavy chain binding protein. Cell 46:291–300. https://doi.org...

  2. 3D Printing Meets CRISPR Cas9

    Type
    Blog Post
    ...Byron Rubin, a crystallographer working with Jane Richardson, provided an initial solution to this problem...problem with the development of Byron’s Bender – a mechanical device that could be used to bend 1/8th inch ... the nascent polypeptide emerges from the exit channel.   He then said “I hadn’t realized how perfectly...

  3. Experimenting with New Careers while in Grad School

    Type
    Blog Post
    ...gave me a place to go once a week to get recharged. The fun change of pace helped break up the negativity...BadAstronomer, Emily Graslie from The Brain Scoop YouTube channel @Ehmee, and David Shiffman @WhySharksMatter and...

  4. Plasmids 101: Secondary Nanobody Toolbox

    Type
    Blog Post
    ... an unusual type of IgG antibody called a heavy chain antibody (HCab), which are unique to camels, llamas...and meticulously screening, optimizing, and characterizing several anti-mouse and -rabbit nanobodies. ...secondary nanobody, it’s important to review a few characteristics of your primary antibody: species it was raised...

  5. INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons

    Type
    Blog Post
    ...CRISPR-associated Transposase) by the Zhang group. Mechanism of CRISPR-transposons One of the CRISPR-transposon...in each type of CRISPR-Transposon system. (B) Mechanism of RNA-guided transposition in type I-F INTEGRATE...bp target-site duplication (TSD), a hallmark characteristic of the Tn7-family of transposons. Insertion...

  6. CRISPR 101: RNA Editing with Cas13

    Type
    Blog Post
    ...adenosine to inosine in RNA, creating a functional A->G change. They fused ADAR deaminase domains (ADARDD) to ...introducing a C opposite the target A to be edited. This change specifies the edit to be made when multiple As ... their RESCUE (RNA Editing for Specific C-to-U Exchange) system (Abudayyeh et al., 2019). To avoid some...

  7. Negative Can Be Positive: Open AAV Data with Addgene

    Type
    Blog Post
    ... peripheral nervous system presents a special challenge to scientists. What works well in one cell or ...vectors in neuroscience Let’s consider my first challenge when I started working with AAV vectors. I wanted...specifically to allow scientists to evaluate how small changes in vector administration can influence AAV expression...

  8. Summer SciComm Series: Modes of Communication

    Type
    Blog Post
    ...graphics for visuals. However, it’s much more challenging to design a hands-on interactive activity using...Flexible. Low barrier to entry. Ample distribution channels both digitally and physically. Easy to incorporate...Media Medium to high Combines pros of multiple channels/balance out cons. Flexible. Can allow people to...

  9. Minigenomes - a Safe Way to Study Dangerous Viruses Like the Ebola Virus

    Type
    Blog Post
    ...investigate viral processes in extreme detail. The mechanism of a particular observation can be easily studied...viral sequences (e.g. promoters) can be studied by changing the minigenome cis-acting sequences and monitoring...24093048. PubMed Central PMCID: PMC3787895. 6. Weik, Michael, et al. "The Ebola virus genomic replication promoter...

  10. Tips for Screening with Yeast Two Hybrid Systems

    Type
    Blog Post
    ...Two hybrid systems were developed in Saccharomyces cerevisiae in 1989 and are still used extensively ...host strain can help. In order to reduce the chance that the configuration of your fusion proteins ...J.L., Boles, E., André, D., and Frommer, W.B. K+ channel interactions detected by a genetic system optimized...

  11. Transferable Skills Guide: External Collaborations

    Type
    Blog Post
    ..., I often found people outside my university approachable, friendly, and helpful. Finally, don’t forget...relationships. Personally, I find this to be the most challenging and time-intensive part of relationship building...people outside your organization just to have a quick chat can be rather tricky (think people based in different...

  12. How to Write a Scientific Review Article

    Type
    Blog Post
    ... and forth with. That being said, we probably exchanged dozens of drafts of the manuscript. This is the...transform the document into something cohesive-- change the sentences, make it flow, and start telling ...Overall, writing a review can be overwhelming and challenging. My best advice is don’t overthink it. And to...

  14. The PAM Requirement and Expanding CRISPR Beyond SpCas9

    Type
    Blog Post
    ... PAMs, respectively (Walton et al., 2020). Characterization of Cas9 from additional bacterial species ...additional Cas9 species PAM sequence may not be characterized Non-SpCas9's bind a variety of PAM sequences.... Furthermore, non-SpCas9's may have other characteristics that make them more useful than SpCas9. For...

  15. Tips for CRISPR Gene Editing in Mice

    Type
    Blog Post
    ...pre-designed plasmids containing these variants ready for purchase. But what is the easiest and fastest way to use...choose the most effective ones to increase your chances of gene modification in vivo. Find Plasmids for...than the delicate RNA and therefore there has less chance of contamination with RNase leading to degradation...

  16. Tips for Using FRET in Your Experiments

    Type
    Blog Post
    ...FRET has also been used to measure conformational changes, cleavage activity using FRET-based biosensors,...method useful for dynamic experiments in which FRET changes are large. Two other methods are commonly used... such as pH or autofluorescence background, can change the fluorescence decay time and have to be taken...

  17. Data Freedom: The Expansion of Data Sharing in Research Publications

    Type
    Blog Post
    ... but sufficiently annotated data used to create charts and tables in the paper are in scope. For images...end” figure or table, clicking on the published chart could reveal the underlying data and allow other...work against transparency. This opens science to charges of elitism and for scientists to be hiding behind...

  18. Quick Guide to Near-Infrared Fluorescent Proteins

    Type
    Blog Post
    ...blue-light controlled optogenetics, such as channelrhodopsins and tools based on light-oxygen-voltage (LOV...tell us about cellular events via  fluorescence changes occurring due to protein synthesis, degradation...developed based on mIFP6. Learn more about the characteristics, performance, and applications of near-infrared...

  19. Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of Neurons

    Type
    Blog Post
    ...OFP (orange), is expressed when Cre is absent; stochastic Cre excision results in one of three recombinations...First, the imaging of Brainbow mouse tissue was challenging due to low fluorescence intensity, caused partially...fluorescent proteins to find those with ideal characteristics (low aggregation, high photostability, and ...

  20. CRISPR 101: Off-Target Effects

    Type
    Blog Post
    ...activates/represses gene expression by various mechanisms. The RNA guide is typically 20 nts and the genomic...off-target events Even after you’ve reduced the chance of off-target effects, you still need to do your... knock-out cell line to perform downstream characterization of, isolating 2 or 3 distinct clones to perform...
Showing: 601 - 620 of 951 results