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We narrowed to 968 results for: lat

Showing: 601 - 620 of 968 results

  1. Plasmids 101: TOPO Cloning

    Type
    Blog Post
    ... Pre-warming your antibiotic-containing plate prior to plating your transformation may allow you to see...TOPO technique specifically uses Vaccinia virus-isolated topoisomerase I as this enzyme recognizes the ...

  2. Comparing Cas9 to NgAgo: Can the Argonautes Best CRISPR?

    Type
    Blog Post
    ...protein could cleave DNA in vitro, directed by phosphorylated DNA guides. Unfortunately, Swarts et al. were...able to mediate homology-directed repair when a template is supplied. Find Plasmids from the NgAgo Publication...in GC-rich regions. DNA guides NgAgo uses 5’ phosphorylated DNA guides (so called “gDNAs”) rather than ...

  3. Plasmids 101: A Brief History of Plasmids and an Improved eBook!

    Type
    Blog Post
    ...plasmid” derived as a hybrid of "cytoplasm" and "id" (Latin for 'it'), as “a generic term for any extrachromsomal...integrated into the chromosome” was proposed a few years later by Élie Jacob and François Wollman and became the...PMCID: PMC427208. Novick, R P et al. “Uniform Nomenclature for Bacterial Plasmids: A Proposal.”Bacteriological...

  5. Another Pathway into Cells: iTOP

    Type
    Blog Post
    ... Primary cells recapitulate the natural biology of a cell type of interest better than immortalized lines...to describe this method of protein transduction. Later studies showed that iTOP functions through macropinocytosis... sorted into single wells to generate clonal populations. After iTOP treatment, both primary (hESCs) and...

  6. Using CRISPR/Cas9 to Edit Disease Out of the Genome

    Type
    Blog Post
    ...targeting the genes responsible for both early onset and late onset AD. In this study, the group showed that CRISPR...Alzheimer’s disease, as well as those that play into late onset AD (5).     CRISPR/Cas9 corrects Alzheimer...CRISPR to disease correction in adult stem cells isolated from two patients with cystic fibrosis. They were...

  7. 25 Work From Home Tips for Scientists

    Type
    Blog Post
    ...at Addgene began working from home last week to flatten the curve, but working remotely can be more difficult...this period, check out these tips and resources collated by Addgenies to help you keep calm and science...local businesses (ex: purchasing a gift card to use later or ordering take-out) or volunteering to foster ...

  8. Creating accessible biology activities in schools with BioBits

    Type
    Blog Post
    ...expensive) the different machines are, and how many regulations are in place to keep everyone safe. None of this...fluorescent protein through transcription and translation. Unfortunately, having live cells in a classroom...hands-on educational activities using the BioBits platform, please get in touch with us! We’ll work with ...

  9. Fluorescent Protein Travel Awards - FLiPs and Fluorescent Protein Biosensors

    Type
    Blog Post
    ...ways to apply fluorescent proteins everyday.  The late Michael Davidson and Roger Tsien played enormous...enzyme active site and shutting off the enzyme. Later exposure to cyan light stopped Dronpa oligomerization...Fluorescent Light-inducible Proteins, or FLiPs.  Xin later optimized these first generation FLiPs by mutating...

  10. How to Lead a Great Meeting

    Type
    Blog Post
    ...communicate outcomes to the rest of their team. Here's a template for project meetings. Team Meetings: For standing...members still have their voices heard. Here's a template for team meetings. One-on-Ones: Managers should... agenda template...

  11. Resume Writing for Non-academic Science Careers

    Type
    Blog Post
    ...people choose a reversed chronological style (the latest to the earliest) or a functional one. Pick one ...when the research topic you have done is closely related to the job you are applying for, in which case ...in Experimental Medicine. She is the Community Relations Director for Curiosity Collider (hyperlink https...

  12. Plasmids 101: Mammalian Vectors

    Type
    Blog Post
    ...be covering this ever-versatile fluorophore in a later post, so stay tuned! Transient transfection and ...transfection A stable transfection is used to create a population of cells that have fully and successfully incorporated... COS-1, 293HEK Inhibits termination step of translation 2-10 ug/mL G418/Geneticin neo HeLa, NIH3T3,...

  13. From Lab Bench to Leadership: Transitioning into Management as a PhD

    Type
    Blog Post
    ...lab or pivot to a managerial role. For those contemplating the leap into management, the journey can be...being a great scientist doesn’t automatically translate to being an effective manager. Mismanaging (no... Kim Scott Courses: Leadership programs on platforms like Coursera, LinkedIn Learning, or edX. Podcasts...

  14. Viral Vectors 101: Gamma-Retroviral Packaging Systems

    Type
    Blog Post
    ...hepatitis virus post‐transcriptional regulatory element; sequence that stimulates the expression of transgenes...-retroviral plasmids have similar names? The nomenclature of the plasmid backbone is meant to be descriptive...

  15. Building and Validating Recombinant Antibodies: A Case Study

    Type
    Blog Post
    ...plasmids for expression of mAbs, the Trimmer lab isolated RNA from cryopreserved hybridomas, immortalized...then used to generate cDNA, which  was used as a template for PCR amplification with a degenerate primer...grown in tissue culture, they were also able to isolate RNA from non-recoverable hybridoma stocks, without...

  16. Hot Plasmids - February 2022

    Type
    Blog Post
    ...miniature CRISPR-Cas system for mammalian genome regulation and editing Anti-FLAG Frankenbodies for modular... genes, researchers at the Genetic Pertubation Platform at the Broad Institute have expanded the genetic...miniature CRISPR-Cas system for mammalian genome regulation and editing by: Rachel Leeson The large size ...

  17. Fluorescent CRISPR Reporters: SRIRACCHA and GEmCherry2

    Type
    Blog Post
    ..., integrated reporter for assaying CRISPR/Cas-stimulated HDR activity) The Madison lab created SRIRACCHA...Joung JK (2014) CRISPR-Cas systems for editing, regulating and targeting genomes. Nature Biotechnology 32...integrated surrogate reporter for assaying CRISPR/Cas9-stimulated homology-directed repair. Journal of Biological...

  18. Developing a cold tolerant E. coli using specialized chaperones

    Type
    Blog Post
    ...contained cold tolerant chaperones (the Cpn 10/60 isolated from Oleispira antarctica, and the P. haloplanktis...psychrophilic systems as well as engineering and regulating psychrophilic recombinant proteins.  To infinity...2019 All-India iGEM Meet-up, which served as a platform for all the Indian iGEM teams to interact with...

  20. A Deep Dive into BioBuilder

    Type
    Blog Post
    ...cellular extracts to perform transcription and translation in vitro, and the new BioBuilder module allows... mentors, and provides a nationally recognized platform in March to exhibit projects at any stage of completion...breakdown, production of fire-resistant housing insulation, and cleaning up salt runoff from winter icing.BioBuilderClub...
Showing: 601 - 620 of 968 results