...for plasmid quality control are: To identify important plasmid features To align NGS sequencing results...Sanger sequencing at Addgene. By using Snapgene’s “Import primers from a list” function, we can detect all...

...your data to a repository (Figshare is currently supported by ReFigure beta) you can include it in a ReFigure... ReFigure team at [email protected] Please support ReFigure by creating ReFigures and answering this...

...hands-on science lab kits and digital curricula to support them. Caitlin’s take on her job as an SME: “I write...communicators: “My recommendation would be to take any opportunity in the lab or outside the lab: write!” Caitlin...

...He debated taking a position as an application support/sales representative in industry or a postdoc in...-on science lab kits and digital curriculum to support them. Many thanks to Hans for taking the time ...

...straightforward workflow for doing gene targeting, reporter generation, controllable gene knockouts, and inducible... models, toolkits like this one provide new opportunities for investigating genetic mechanisms related...

...do. From all of us to all of you. Thanks for supporting nonprofit, open science and Addgene in our mission...Collaboration with Fellow Nonprofit Seeding Labs Addgene Supports Rare Disease Research Listen to Our Interview ...

...nuclear localization signals (NLS) increase protein import into the nucleus. In developing their SunTag technology...protein function, thereby creating a single-molecule reporter of intracellular processes. Initially, Tanenbaum...

...starting a cascade of events that results in the pores being opened in the pathogen’s cell membrane (Dunkelberger... when holes are punched in their membranes, the pore'd pathogens quickly lyse and die.  Antibody dependent...

...capsid, “pumps” the genome into the capsid through a pore in the capsid. Rep proteins are also involved in...2020) Adeno-Associated Virus Genome Interactions Important for Vector Production and Transduction. Human ...

...Product Run PCR to amplify your insert DNA. It is important to use a high fidelity taq polymerase to minimize.... The fidelity of the polymerase becomes more important the longer the expected PCR product is. You should...temperature based on the melting temperature (Tm) of the portion of the primer that hybridizes to the sequence to...some DNA during the gel purification step, it is important to digest plenty of starting material. We recommend...be cut with both enzymes, and therefore it is important that the digest goes at least 4 hours and as long...running a gel for purification purposes it is important to have nice crisp bands and to have space to ...expected. When cloning by PCR, it is especially important to run the product on a gel. This allows you to...

...446 Benzamide, Millipore Sigma 12072 Antipain, Millipore Sigma 10791 Leupeptin, Millipore Sigma L2884 Aprotinin...monobasic monohydrate, Millipore Sigma 71507-250G Sodium phosphate dibasic, Millipore Sigma S7907-500G 1 ...that sharing the full details of our protocols supports reproducibility and accelerates science. Here,...information is solely for transparency intended to support reproducibility in science. Safety Warnings Sodium...1 M Trizma hydrochloride solution pH 9.0, Millipore Sigma T2819-1L 250 mL sterile bottle, VWR 430281 0.45... Aprotinin, Millipore Sigma A6279 Zeba Spin Desalting Columns, 7 kDa MWCO, 10 mL, Thermo Fisher 89893 ...89893 Amicon Ultra-15 30 kDa MWCO column, Millipore Sigma UFC903024 50 mL LoBind tubes, VWR 76289-498 Before...

...enhanced GFP (EGFP)-type termini. mNeonGreen was reported as the brightest monomeric green or yellow fluorescent... or for live cell imaging of fusions and FRET reporters. mTFP1 is also insensitive to physiologically ...

...through manuscripts, seminars, or posters. It is important to note that it is the actual communication itself...quickly!  The question   The first, and most important question, you should ask for any science communication...

...Working on the scientific support team at Addgene, I get to see everything that can go wrong with a plasmid...Growth Conditions To reduce recombination, it is important to not overgrow the plasmid at 37°C. Some researchers...

... assay, the pseudoviruses express a luciferase reporter which is used as a readout for infection of ACE2...neutralize the virus,” says Balazs, emphasizing the importance of the second vaccine dose in mounting a robust...

... new services. “We appreciate NIH’s vision and support for this project. This grant will allow Addgene... part of this initiative! The project will be supported by a NIH BRAIN Initiative grant (NS119916). The...

... of being honest and straightforward, it’s an important part of being a “good scientific citizen” and ...Press journals, including Cell Metabolism, Cell Reports and Joule. In September 2017 she moved away from...

...Gerald Schwank, first author of the cystic fibrosis report. “This is a big advantage over induced pluripotent...and many others. “Those off-target effects are important,” she said. “We also really need to get a handle...

...other areas of scientific research. We continue to support those involved in COVID-19 research, but our new...make science a better place. You will receive opportunities to collaborate with other scientists and to ...

...be shared with others. The Web Importer plug-in lets you directly import papers from web pages and academic...