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TypeBlog Post... sure to include various positive and negative controls to “convince [any] old skeptic professor who's...conference is a big multidisciplinary one.” Cepko strongly emphasizes that people should “follow their passions...
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In Living Color: The Skinny on In Vivo Imaging Tools
TypeBlog Post... light in the near-infrared portion of the electromagnetic spectrum – the spectral region in which mammalian...Nature Communications last year, “The capability to control spectral properties of PA FPs with light of specific... -
We're Updating Our Hold for Publication Policy
TypeBlog Post...policy gives us time to complete necessary quality control and legal approvals so the plasmids are ready for...start out with this hold! You will always have control over when your plasmids are distributed. Next month... -
Addgene-Seeding Labs Plasmid Grant: Accelerating Science Globally - 5 Scientists, 4 Countries & 3 Continents!
TypeBlog Post...researchers in developing countries. We are proud to introduce the 5 scientists who were awarded the Addgene-...technology to engineer plants that are able to destroy DNA viruses, similar to the anti-phage CRISPR effect... -
Fluorescent Proteins 101: Fluorescent Biosensors
TypeBlog Post...a reporter signal. Reporter signals may be electrochemical or light-based, with luminescent and fluorescent... being especially popular. We’ll give you an introduction to fluorescent biosensors, but keep in mind ... -
New CRISPR Web Resources and #12DaysofCRISPR Recap
TypeBlog Post...Each plasmid page now has a helpful graphic to introduce you to the plasmid class covered on that page....Using CRISPR/Cas9 and Plasmids 101: NGS Quality Control for Pooled Libraries Day 12: New website resources... -
Antibodies 101: Affinity Tags
TypeBlog Post...You’ve designed the perfect experiment – controls, conditions, and everything in between – now all you... Susanna Stroik... -
Hot Plasmids September 2018 - Optogenetics, RNA Localization, Fluorescent Protein, and Base-editing Tools
TypeBlog Post... Listen this episode of Hot Plasmids! Light controls the activity of a microtubule-associated protein...studying cellular dynamics. The photoinducible control of protein function is more specific and much faster... -
The Golden Stopwatch Award: Recognizing Facilitators of Research
TypeBlog Post...packed with tech transfer resources (summaries, electronic MTA approval, archive of old MTAs, and more) ... Transfer Information Tech Transfer News: A Reintroduction to the UBMTA ... -
Single-cell tracking of lineage and identity with CellTag
TypeBlog Post...cell types. MEFs were reprogrammed into iEPs by retroviral expression of transcription factors Foxa1 and.... Adding Mettl7a1 to the standard Foxa1-Hnf4α retroviral overexpression cocktail yielded a three-fold ... -
CRISPR Challenges: Standardization and Homology Directed Repair
TypeBlog Post...surprised to see that it would open not with an introduction to CRISPR nucleases, but with a session on DNA...editing rates across multiple systems. Read our introductory blog post, then check out this RNP protocol ... -
Fluorescent Biosensors for Measuring Autophagic Flux
TypeBlog Post... A fluorescent probe that releases an internal control Another autophagy biosensor is the GFP-LC3-RFP-LC3ΔG...it remains in the cytosol acting as an internal control. Autophagic flux is estimated by the GFP/RFP ratio... -
Antibodies 101: Avoiding the Mouse on Mouse Mess in IHC
TypeBlog Post...endogenous IgGs, you should include no primary antibody controls in your experiments. If you do find high levels...resources on the Addgene blog Antibodies 101: Introduction to Immunofluorescence Antibodies 101: Affinity... -
What the HEK?
TypeBlog Post...Culture Adenoviral Vector Production and Troubleshooting Additional resources on Addgene.org AAV ... Susanna Stroik... -
Even more elegant: Single injection CRISPR/Cas9 in C. elegans
TypeBlog Post...elegans, they inserted the SEC into a synthetic intron between a fluorescent protein (FP) and a 3xFLAG...resulting loxP scar is located in this synthetic intron, and only the fluorescent protein and 3xFLAG-tag... -
Advice for Moving Into Sales After Your Science Postdoc
TypeBlog Post...build, sales and marketing gives you a great introduction to the financial world. It teaches you about...you can find some great information on ELISA troubleshooting, ELISA kits and cell death assays. You can ... -
Pushing the Limits of DNA Assembly
TypeBlog Post...necessarily have to be. Regions of homology can be introduced to fragments via PCR using primers that contain...patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc. Many thanks to ... -
Scientists Map the SARS-CoV-2-Human Interaction Network
TypeBlog Post...Nsp5. Our set also includes a GFP construct as a control. For easier portability, all viral genes are flanked...clone selection. Expression is driven by a fairly strong EF1alpha promoter. We have verified expression ... -
25 Work From Home Tips for Scientists
TypeBlog Post... one figure. If you aren’t there yet, write introductions to potential publications or prepare a review...isn’t for a while. Getting started early on introductions and literature reviews could make the months... -
Creating accessible biology activities in schools with BioBits
TypeBlog Post...build circuits in physics class to learn about electronics or mix chemicals together in chemistry to see... way into high school classrooms, like gel electrophoresis to separate DNA or transforming cells with ...