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Showing: 601 - 620 of 922 results
  1. 9 Tips to Achieve Success in Academia

    Type
    Blog Post
    ... sure to include various positive and negative controls to “convince [any] old skeptic professor who's...conference is a big multidisciplinary one.” Cepko strongly emphasizes that people should “follow their passions...
  2. In Living Color: The Skinny on In Vivo Imaging Tools

    Type
    Blog Post
    ... light in the near-infrared portion of the electromagnetic spectrum – the spectral region in which mammalian...Nature Communications last year, “The capability to control spectral properties of PA FPs with light of specific...
  3. We're Updating Our Hold for Publication Policy

    Type
    Blog Post
    ...policy gives us time to complete necessary quality control and legal approvals so the plasmids are ready for...start out with this hold! You will always have control over when your plasmids are distributed. Next month...
  4. Fluorescent Proteins 101: Fluorescent Biosensors

    Type
    Blog Post
    ...a reporter signal. Reporter signals may be electrochemical or light-based, with luminescent and fluorescent... being especially popular. We’ll give you an introduction to fluorescent biosensors, but keep in mind ...
  5. New CRISPR Web Resources and #12DaysofCRISPR Recap

    Type
    Blog Post
    ...Each plasmid page now has a helpful graphic to introduce you to the plasmid class covered on that page....Using CRISPR/Cas9 and Plasmids 101: NGS Quality Control for Pooled Libraries Day 12: New website resources...
  6. Antibodies 101: Affinity Tags

    Type
    Blog Post
    ...You’ve designed the perfect experiment – controls, conditions, and everything in between – now all you... Susanna Stroik...
  7. Single-cell tracking of lineage and identity with CellTag

    Type
    Blog Post
    ...cell types. MEFs were reprogrammed into iEPs by retroviral expression of transcription factors Foxa1 and.... Adding Mettl7a1 to the standard Foxa1-Hnf4α retroviral overexpression cocktail yielded a three-fold ...
  8. Fluorescent Biosensors for Measuring Autophagic Flux

    Type
    Blog Post
    ... A fluorescent probe that releases an internal control Another autophagy biosensor is the GFP-LC3-RFP-LC3ΔG...it remains in the cytosol acting as an internal control. Autophagic flux is estimated by the GFP/RFP ratio...
  9. Antibodies 101: Avoiding the Mouse on Mouse Mess in IHC

    Type
    Blog Post
    ...endogenous IgGs, you should include no primary antibody controls in your experiments. If you do find high levels...resources on the Addgene blog Antibodies 101: Introduction to Immunofluorescence Antibodies 101: Affinity...
  10. What the HEK?

    Type
    Blog Post
    ...Culture Adenoviral Vector Production and Troubleshooting Additional resources on Addgene.org AAV ...  Susanna Stroik...
  11. Advice for Moving Into Sales After Your Science Postdoc

    Type
    Blog Post
    ...build, sales and marketing gives you a great introduction to the financial world. It teaches you about...you can find some great information on ELISA troubleshooting, ELISA kits and cell death assays. You can ...
  12. Pushing the Limits of DNA Assembly

    Type
    Blog Post
    ...necessarily have to be. Regions of homology can be introduced to fragments via PCR using primers that contain...patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc. Many thanks to ...
  13. Scientists Map the SARS-CoV-2-Human Interaction Network

    Type
    Blog Post
    ...Nsp5. Our set also includes a GFP construct as a control. For easier portability, all viral genes are flanked...clone selection. Expression is driven by a fairly strong EF1alpha promoter. We have verified expression ...
  14. 25 Work From Home Tips for Scientists

    Type
    Blog Post
    ... one figure. If you aren’t there yet, write introductions to potential publications or prepare a review...isn’t for a while. Getting started early on introductions and literature reviews could make the months...
Showing: 601 - 620 of 922 results