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...Culture Adenoviral Vector Production and Troubleshooting Additional resources on Addgene.org AAV ...  Susanna Stroik...

...elegans, they inserted the SEC into a synthetic intron between a fluorescent protein (FP) and a 3xFLAG...resulting loxP scar is located in this synthetic intron, and only the fluorescent protein and 3xFLAG-tag...

...build, sales and marketing gives you a great introduction to the financial world. It teaches you about...you can find some great information on ELISA troubleshooting, ELISA kits and cell death assays. You can ...

...necessarily have to be. Regions of homology can be introduced to fragments via PCR using primers that contain...patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc. Many thanks to ...

...system, and cell turnover. Apoptosis is a highly controlled process that is triggered by internal and external...spatial and temporal dynamics of apoptosis both in vitro and in living organisms to better study and understand...

...build circuits in physics class to learn about electronics or mix chemicals together in chemistry to see... way into high school classrooms, like gel electrophoresis to separate DNA or transforming cells with ...

...YOUR PLASMID has been developed to teach gamma-retrovirus or lentivirus-based ex-vivo gene therapy’ strategies...at FRESCI particularly interested in advanced in vitro models technology. Follow him on twitter @FRE_SCI...

...person or virtually) will make the relationship stronger.  If appropriate, invite them to speak for your...chocolate-covered strawberries for helping with an introduction that helped someone get a job interview. Even...

...Nsp5. Our set also includes a GFP construct as a control. For easier portability, all viral genes are flanked...clone selection. Expression is driven by a fairly strong EF1alpha promoter. We have verified expression ...

... one figure. If you aren’t there yet, write introductions to potential publications or prepare a review...isn’t for a while. Getting started early on introductions and literature reviews could make the months...

...complex genetic diseases, Alzhiemer’s disease (AD).  Troy Rohn and his lab are researching a way to treat ...proteins aid the limitation of these mutations, to control the CRISPR system activity.  Using an AAV vector...

... the lab, so in December 2015 we held our first intro to bacterial transformation course (read our blog...bacterial transformation guru and instructor for our intro to biohacking courses. Our members pursue a diverse...

...you did. Emphasize the skills. If you studied astronomy or bioinformatics data, then the chance is that...Communications Coordinator for the Department of Physics & Astronomy, University of British Columbia. Originally on...

...sequence. Here at Addgene, we use NGS-based quality control to confirm the sequence of all the plasmids we ...ladder based on the expected band sizes. Always run control uncut DNA to ensure your enzymes are working. When...

...equipment always helps. But how does one build a strong collaborative research team motivated by similar...selectively picking the first couple of hires to build a strong, collaborative team. And take advantage of the ...

...extracts to perform transcription and translation in vitro, and the new BioBuilder module allows students to...Assembly Standards Find Plasmids for Creating and Controlling Gene Networks Browse All SynBio Plasmids ...

...presented Opto-CRAC which allows a researcher to control immune signaling and inflammatory responses remotely...deposit and distribution service! References: 1. Pietrocola, Federico, et al. "Caloric restriction mimetics...

...minimally informative. In this blog post, we’ll introduce digital PCR as an emerging validation technology...assays for mutation screening Now that we have introduced how digital PCR works, let’s explain what kind...

...team develops methods, performs extensive quality control, generates  educational materials, and provides...details about virus production protocols and quality control can be found on Addgene’s Viral Production webpage...

...improved RNAi vector that leads to significantly stronger phenotypes than standard RNAi protocols without...domain family, known to be involved in cytoskeletal control, cell growth, and endocytosis. Recently, the Sidhu...