We narrowed to 972 results for: Abo;

1. Viral Vectors 101: Producing Your rAAV

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   ...dehydration (Howard & Harvey, 2017). You can learn more about common AAV applications by reading our Viral Vectors...

2. PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs

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   ... al., 2023). We trained our models in close collaboration with the Krauthammer lab (University of Zurich...

3. Twenty Years of Addgene Sharing: CRISPR

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   ...with Addgene by year. (C) Cumulative number of laboratories that have deposited CRISPR plasmids by year....

4. Hot Plasmids and Viral Preps - May 2021

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   ...acid, suggesting that MPyV system can improve metabolic pathway yields by encapsulating a single enzyme...

5. Light Sheet Fluorescence Microscopy

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   ...protein (GFP) (6).   The Dodt and Frank Bradke laboratories later developed the 3DISCO (3-dimensional imaging...

6. What’s in Your Tube?: A Quick Guide to Measuring DNA by Spectrophotometry

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   ...flow-through solutions and use clean tubes? How about spin times, were these long enough?  There are, ...

7. Antibodies 101: Producing Recombinant Antibodies

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   ...validated, it’s ready for use! You can learn more about common antibody applications in our Antibodies 101...

8. Minigenomes - a Safe Way to Study Dangerous Viruses Like the Ebola Virus

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   ...Microbiology, National Emerging Infectious Disease Laboratories.  She is interested in genome replication and...

9. An Introduction to Adenovirus

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   ...this introduction gives a sense of what AdVs are about – and we hope that it inspires further use of existing...

10. Getting to Know Expansion Microscopy

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    ...applications and there’s just not much to be done about that.  Second, expansion introduces distortions....

11. Uncovering Ancestral Bias in CRISPR Research

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    ...dependencies associated with each ancestry group. Since about 90% of the cell lines used in DepMap CRISPR screens...

12. Negative Can Be Positive: Open AAV Data with Addgene

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    ...asked why they don’t share, many express concern about sharing negative data they cannot explain, perhaps...

13. INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons

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    ..., 2020), to multi-copy genomic integration of metabolic cassettes (Zhang et al. 2020); however, the technology...

14. Interview: Hodaka Fujii on enChIP, New CRISPR Tools, and More

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    ...University of Tokyo, Osaka University). Can you speak about your different experiences in research labs. What...

15. "Hall of Fame" AAV Enhancers from the Allen Institute for Brain Science

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    ...Collection and the BRAIN Armamentarium Collection, a collaborative effort that the Allen Institute contributes ...

16. Harnessing TnpB for Genome Editing: A Compact and Versatile Tool for Your Research

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    ...precise and accessible genome engineering. More elaborate protocols are available in our earlier publications...

17. QC Sequencing Technologies at Addgene

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    ...sequencing was considered the gold standard until about 2008 when a new, higher-throughput sequencing-by-synthesis...

18. Antibodies 101: The Four ELISAs and When to Use Them

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    ...conjugate antibodies themselves, the process can be laborious and expensive. In some cases, conjugation methods...

19. Hot Plasmids - November 2023

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    ...section of the Addgene antibody page, and learn more about the whole-brain reference atlas efforts, including...

20. Viral Vectors 101: Preparing Pooled Libraries

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    ...ordering a pre-made library, you don’t need to worry about amplifying and producing lentivirus. But pre-made...

21. AAV Titers: Where Do They Come From And What Do They Mean?

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    ... Addgene.org Find All Available AAV Learn More about Virus Production Find Viral Vector Protocols ...

22. Plasmids 101: Broad Host Range Plasmids

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    ...plasmids may have high copy numbers and reduced metabolic load (Meyer 2009). Figure 1: Idealized ...

23. New Acoustic Reporter Genes: Ultrasound Imaging of Gene Expression

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    ...you enjoy playing with our genes. Have fun!    About the authors: Marjorie Buss is a PhD student studying...

24. New CRISPR Tools: Cas7-11 and PASTE

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    .... Eugene Koonin and Kira Makarova, longtime collaborators of Omar and Jonathan and experts in CRISPR evolution...

25. Viral Vectors 101: Pseudotyping

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    ... also toxic at high concentrations as described above and is inactivated by the complement system in serum...

26. Rett Syndrome: A History of Research and Therapeutic Outlooks

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    ...therapy. In 2014, RSRT brought together a strong collaboration between two gene therapy labs (Brian Kaspar ...

27. Plasmids 101: Expressing shRNAs for RNA Interference

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    ... & Corey, D. R. (2021). Reexamining assumptions about miRNA-guided gene silencing. Nucleic Acids Research...

28. Hot Plasmids: Summer 2024

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    ...transcriptional silencing (Nuñez et al., 2021). Now, in a collaboration with the Vallabh/Minikel Lab, the Weissman Lab...

29. CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9

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    ... Addgene. In addition to the plasmids described above, Addgene carries several useful CRISPR toolkits ...

30. Site Directed Mutagenesis by PCR

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    ... Brush up on Your Cloning Techniques Learn More about Plasmids in Our Plasmids 101 Series Use CRISPR to...