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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 1,045 results for: Abo;

Showing: 621 - 640 of 1045 results

  2. Behind the scenes: Addgene’s new search engine and more

    Type
    Blog Post
    ...this multidisciplinary effort and cross-team collaboration (thanks to all Addgenies involved). The new ...Additional resources from the Addgene blog Learn about other features on Addgene's website Find a step-...to using Addgene's new search Read to learn more about why we redesigned our homepage and mascot ...

  4. Visualizing Protein Turnover In Situ

    Type
    Blog Post
    ... FUNCAT Professor Schuman’s technique applies metabolic labeling using a noncanonical amino acid, azidohomoalanine...both quantitative and spatio-temporal information about the turnover of specific endogenous proteins in ... interested in neurobiology.  She is passionate about communicating science to all audiences. Right now...

  5. Giving gRNAs a Facelift - Synthetic and Beyond

    Type
    Blog Post
    ...“I hope it isn’t degraded” when scientists talk about their precious RNA samples. RNA is inherently less...efficacy as well as the unnecessary stress of worrying about the status of your RNA. gRNAs modifications are ...Some of the RNA stabilizing modifications listed above were developed before CRISPR-Cas9, for use in applications...

  6. Antibodies 101: Conjugation

    Type
    Blog Post
    ...Check out our post on Biotinylation to learn more about biotin and its use in molecular biology. Other Conjugates...perfectly blissful, productive lives without thinking about these chemical reactions. After all, there is an...ready to use off the shelf. So why are we talking about this? Even though there are so many commercially...

  7. Viral Vectors 101: An Introduction to AAV

    Type
    Blog Post
    ...make AAV ideal for human therapies and in vivo laboratory experiments ranging from transient gene expression...genetic cargo. Typically, when researchers speak about AAVs, they are actually referring to rAAVs. ITRs...Primer for Beginners Read Our Lentivirus FAQ Learn about Viral Vector Elements Resources on Addgene.org ...

  8. Plasmids 101: The protein expression toolbox

    Type
    Blog Post
    ...which can be used with this approach - read more about them on our CRISPRa blog! Fig. 1 CRISPR ... is a lot of overlap with the systems discussed above, just with more elements of control. Tet promoters...to be site-specifically knocked in. This can be labor intensive and time consuming. If your native protein...

  9. CRISPR 101: Cas9 vs. The Other Cas(s)

    Type
    Blog Post
    ... is smaller than the traditional SpCas9 enzyme (about a third of the size) and does not require a tracrRNA...Cas12a to further optimize it for therapeutic and laboratory usage. Fast facts – PAM requirement: TTN or ... the lack of collateral cleavage). You can read about Cas7-11 in our blog post on its development and ...

  10. Antibodies 101: Selecting the Right Antibody

    Type
    Blog Post
    ...Narrowing the field After working your way through the above steps, you may find that you still have a number...advice if you need to troubleshoot. What do you know about the vendor and the vendor’s technical support? An...validation is equal and you should think critically about what data is and is not shown on an antibody website...

  12. Antibodies 101: Polyclonal Antibodies

    Type
    Blog Post
    ...  Read about another common type of antibody for research, monoclonal antibodies Learn about the advantages...Once the initial antibody response starts to wane about a month later, the animal is given additional booster...

  13. Supporting Rare Disease Research with the 2020 BeHEARD Awards

    Type
    Blog Post
    ...Deficiency (CTD) is a rare X-linked inherited neurometabolic condition and one of three Cerebral Creatine... into CCDS. The brain and muscles have a high metabolic demand that is powered by ATP. A molecule central...Hébert's lab. Additional Resources Find out more about the Rare Genomics Institute and this year's BeHeard...

  14. RNA Extraction Without A Kit

    Type
    Blog Post
    ...more foolproof (still without a kit!) As mentioned above, working with RNA requires keeping your samples ...lysis. This can be challenging depending on your laboratory situation or tissue collection method, so biotechnology...supernatant is extracted with chloroform as in the above no-kit method.  Researchers have also developed ...

  15. Enhancing CRISPR Targeting Specificity with eSpCas9, SpCas9-HF1, & HypaCas9

    Type
    Blog Post
    ...With this new found structural knowledge, in collaboration with the Joung Lab, they used targeted mutagenesis...edits in mammalian cells and may decrease worries about off target effects in applied and/or therapeutic...at Addgene. Resources on the Addgene Blog Read about Cas9 Homolog Cpf1 Read How Non-Homologous End Joining...

  16. New Tool for Lineage Tracing: The ClonTracer Library

    Type
    Blog Post
    ...process.” The ClonTracer library originally came about as Bhang and co-workers were trying to develop a...particularly interested in applying what we have learned about tumor heterogeneity to drug discovery and the development...Additional Resources on the Addgene Blog Learn about the Sabatini and Wood Drug Screening Kit Find Out...

  17. Writing Scientific Manuscripts: Literature Searching, Reading, & Organizing

    Type
    Blog Post
    ...interpretation of the results? Then think deeper about the data they are showing. Are those experiments...points of the protocols. Are there any red flags about how the work was performed that could influence ...University. She is a fan of photosynthesis and writes about autotrophs and biochemistry on her blog. Follow ...

  18. Popular Retroviral Vectors and Their Uses in Scientific Research

    Type
    Blog Post
    ...of their choice in mammalian cells.  Learn more about Mammalian shRNA Tools for RNAi! Cre-Lox technology...knockdown. Vector provided by the Qi lab. Learn more about CRISPR plasmids for transcriptional inhibition. .... Check our CRISPR guide and blog to learn more about CRISPR and the most recent developments in the genome...

  19. BeHeard Award 2018: Diseases of Glycosylation, Arginine Mutagenesis, & Neural Development

    Type
    Blog Post
    ...glycosylation (CDG) are a group of rare inherited metabolic diseases with severe and life-threatening consequences...understood.  In line with this, Prof. Videira and collaborators will use Addgene plasmids to immortalize CDG...discovery led them to investigate the use of a common laboratory chemical, guanidine hydrochloride, as a potential...

  20. Adeno Associated Virus (AAV) for Cell and Gene Therapy

    Type
    Blog Post
    ...Vectors Featured Topic Page Learn about CRISPR-AAV vectors Learn about Addgene AAV Quality Control Process...viral infections. She is particularly passionate about HIV cure research and science communication.     ...
Showing: 621 - 640 of 1045 results