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TypeBlog Post...mutation on the receptor ACVR1. ACVR1 triggers appropriate bone regeneration in healthy patients. However...antibody that binds Activin A and interferes with inappropriate activin-ACVR1 interactions prevents FOP bone...
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Management for Scientists: Managing vs. Leading
TypeBlog Post...managers and some take on leadership roles. You may be happy with a primary role of doer for your entire career...they miss the hands-on bench work. To really be happy, you may need to find a way to do, manage and lead... -
Management for Scientists: Giving Feedback
TypeBlog Post...respond to the employee’s level of discomfort appropriately to get the message across. Listen to our podcast...to you. What are We Learning: What would have happened if _________? What would have been a better outcome... -
Addgene @ Keystone: Thoughts on Precision Genome Engineering and Synbio
TypeBlog Post...guarantee that their experiments will work (yes, this happens): Science is hard. Chris Voigt, another Addgene...every conceivable background at the meeting. I appreciated all the feedback I received from scientists, ... -
Plasmids 101: Antibiotic Resistance Genes
TypeBlog Post...and can be used in place of Ampicillin in most applications. Antibiotics vary in their sensitivitly to heat...hotter than about 55oC and store plates/stocks wrapped in foil if a light-sensitive antibiotic like Tetracycline... -
HA Frankenbody, a New Imaging Tool to Visualize Single Molecules and Nascent Peptides
TypeBlog Post... the future, this stitching technique could be applied to other epitopes and scaffolds to build a more...antibodies for extracellular and intracellular applications: CDR grafting to stable frameworks and structure-based... -
Single-cell tracking of lineage and identity with CellTag
TypeBlog Post...not silenced. Find CellTag libraries here! Applications of CellTag The Morris Lab developed CellTag ...References Biddy, Brent A., et al. "Single-cell mapping of lineage and identity in direct reprogramming... -
New and Upcoming Viral Vectors - Spring 2019
TypeBlog Post...kinetics) that makes them suitable for different applications. We recently released a new variant of jGCaMP7... been developed and are suited for different applications. The iGABASnFR GABA sensor (pAAV.hSyn-FLEX.iGABASnFR... -
Arabidopsis in Education: How the Arabidopsis Biological Resource Center Brings Plant Science to Life
TypeBlog Post... Biological Resource Center and the Center for Applied Plant Sciences at The Ohio State University. Established... Biological Resource Center and the Center for Applied Plant Sciences at The Ohio State University. In... -
CRISPR Challenges: Standardization and Homology Directed Repair
TypeBlog Post...the gRNA design process and rules for various applications is necessary going forward. The standardization...Alt-NHEJ), has been harnessed for CRISPR knock-in applications. CRISPR 101: Targeting RNA with Cas13a (C2c2... -
Identifying Sequence Elements with SnapGene's Feature Database
TypeBlog Post...response to customer feedback. An example of this approach is SnapGene’s algorithm for detecting common features.... We are working on a way for SnapGene users to apply features from a reference strain to newly sequenced... -
What Do I Do Now? Academic v. Non-Academic Career Decisions
TypeBlog Post...can do better, no grant writing Job changes can happen regularly (every 4-6 years is common), but this...issues can create interpersonal issues (one bad apple can change entire culture) Specific requirements... -
Summer SciComm: Science Storytelling
TypeBlog Post... work. And while these pieces aren't always appropriate for your formal scientific communications, they...still get invested in the journey? The same thing applies for science storytelling. You can introduce your... -
Antibodies 101: Epitope Availability
TypeBlog Post...categories: ones induced by the researcher and ones that happen naturally. Sample Denaturing First, let’s think...SDS-page gel. As the protein unfolds, new epitopes may appear, while others may get hidden. Monoclonal antibodies... -
Antibody Neutralization Response Against Pseudoviruses Expressing SARS-CoV-2 Spike Protein Variants
TypeBlog Post...surprising was the extent to which the escape appeared to be happening.” In their assay, the neutralization ... -
Viral Vectors 101: What is a Virus (and a Viral Vector)?
TypeBlog Post...into two cells. Viral vectors Viruses are quite appealing as molecular biology tools… if one isn’t too bothered...on the Addgene blog Viral Vectors 101: Viral Applications Viral Vectors 101: Viruses as Biological Tools... -
Plasmids 101: TOPO Cloning
TypeBlog Post...“A” overhang insert, the magic of TOPO cloning happens. Basic procedure Let’s break down the steps needed...1658796. PubMed Central PMCID: PMC52876. 2. Novel approach to molecular cloning and polynucleotide synthesis... -
Donations from Addgene to Yield Answers for Rare Disease Researchers
TypeBlog Post...on OCRL1 already underway in his lab. The gene appears to play a role in many cell processes involving...In Thailand, our annual operating budget is approximately 20,000 USD and we are working on several genetic... -
Don’t Wait To Launch Your Health Science Startup
TypeBlog Post...would apply a percentage of returns to support student projects, but it quickly became apparent that raising... -
Another Pathway into Cells: iTOP
TypeBlog Post...protein-only transduction method and its potential applications to CRISPR/Cas9 genome editing. Protein-only...Diego D'Astolfo. CRISPR/Cas9: a potential application of iTOP Having tested iTOP using Cre-lox recombination...