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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 964 results for: App;

Showing: 621 - 640 of 964 results

  2. New Norepinephrine Indicators: nLightG and nLightR

    Type
    Blog Post
    ...neurons expressing nLightG or nLightR before/after application of NE (10 μM) and corresponding pixel-wise ΔF...GFP), and nLightR uses a red fluorescent protein, mApple. Kagiampaki et al. found nLight is suitable for...

  4. 9 Tips to Achieve Success in Academia

    Type
    Blog Post
    ...emphasizes that people should “follow their passions, be happy, and match their personal goals to their career ...analytical and critical thinking skills that are applicable to a variety of careers outside of academia. ...

  5. Virus Protocol - Generating Stable Cell Lines

    Type
    Protocol
    ...expression observed using transient transfection approaches, generating cell lines using lentiviral vectors... tubes, VWR 21008-216 Lentivirus preparation Appropriate antibiotic for selection (e.g. puromycin, blasticidin...lower dilutions depending on your downstream applications. If you’ve titered your virus beforehand, you.... Add 1.5 mL of DMEM complete containing the appropriate antibiotic. This is the beginning of the selection...

  6. CASTing Off for New Shores in Human Genome Editing

    Type
    Blog Post
    ...individualized optimizations, safety testing, and approval. In contrast, a system that can deliver a healthy...could provide a streamlined, one-size-fits-most approach. evoCAST is an important step toward that kind...

  8. Fostering communication in remote collaborations

    Type
    Blog Post
    ...willingness of the team to adopt various tools, this may happen over email or using rapid communication tools such...access data and drafts of manuscripts and grant applications in real-time so they can provide input at the...

  9. Bringing Sustainable Practices to the Lab: Recycling

    Type
    Blog Post
    ... at least we can reduce the rate at which that happens and reduce the manufacturing of new containers,...bin or box in your tissue culture room for the wrappers and ship them back. Start a glove recycling ...

  10. Running for Rare Disease, Running for FOP, Running for AJ

    Type
    Blog Post
    ...mutation on the receptor ACVR1. ACVR1 triggers appropriate bone regeneration in healthy patients. However...antibody that binds Activin A and interferes with inappropriate activin-ACVR1 interactions prevents FOP bone...

  11. Management for Scientists: Managing vs. Leading

    Type
    Blog Post
    ...managers and some take on leadership roles. You may be happy with a primary role of doer for your entire career...they miss the hands-on bench work. To really be happy, you may need to find a way to do, manage and lead...

  12. Management for Scientists: Giving Feedback

    Type
    Blog Post
    ...respond to the employee’s level of discomfort appropriately to get the message across. Listen to our podcast...to you. What are We Learning: What would have happened if _________? What would have been a better outcome...

  14. Plasmids 101: Antibiotic Resistance Genes

    Type
    Blog Post
    ...and can be used in place of Ampicillin in most applications. Antibiotics vary in their sensitivitly to heat...hotter than about 55oC and store plates/stocks wrapped in foil if a light-sensitive antibiotic like Tetracycline...

  15. Affinity Purification of Recombinant Antibodies with Protein A or Protein G

    Type
    Protocol
    ...bottom. Add 5 mL of Pierce IgG Elution Buffer to the capped Gravitrap column and incubate for 10 min at RT...subsequent centrifugation step Pro-Tip Resin will appear compacted after centrifugation. Discard the flow...column in a new 50 mL LoBind collection tube. Slowly apply the sample to the center of the compact resin bed...exchange and concentration with an Amicon column Apply 15 mL of PBS to the reservoir of a Amicon Ultra-...

  19. Viral Vectors 101: What is a Virus (and a Viral Vector)?

    Type
    Blog Post
    ...into two cells. Viral vectors Viruses are quite appealing as molecular biology tools… if one isn’t too bothered...on the Addgene blog Viral Vectors 101: Viral Applications Viral Vectors 101: Viruses as Biological Tools...
Showing: 621 - 640 of 964 results