We narrowed to 658 results for: CHI

... or blog on optimizing this step, or consider enriching for transfected cells by flow sorting or drug ...

...within a cell,” “Does this disease change the architecture of my cells,” or “How does this mutation impact...

...your particular setup. We recommend starting by searching the literature to see what is already known about...

...expression, and viability. Gates are the parameters the machine uses to differentiate between variations in those...

... stimulate and study these physical processes. Joachim Goedhart and colleagues in the Gadella Lab devised...

...usually see a director sitting in their chair not watching their actors live on set, but peering at a 7-inch...

...purify proteins from E. coli, Bacillus subtilis, or Pichia pastoris depending upon your specific protein folding...

... introduce a frame-shift mutation. This can be achieved using a restriction enzyme that recognizes a unique...

...hurdles. The first is the delivery of the CRISPR machinery to all of the target cells. This goal may prove...

...editing faces the challenges above and more. One chief challenge is upregulating HDR in mature tissues,...

...process of replicating your new plasmid: the Escherichia coli bacteria. It’s hard to count the number ...

... harboring large inserts are often dimers in Escherichia coli K-12. Plasmid. 1989 Mar;21(2):138-41. doi...

... it’s important to know what you are aiming to achieve. In general, these are three core goals of antibody...

...target site (Fig. 3B). The INTEGRATE system can achieve ~100% integration of DNA up to 10 kB in size in...

...researchers, as well as graduate students (your teaching assistants), about their experiences. Research...

... molecules. FRET sensors face challenges of photobleaching, autofluorescence, and, in the case of exciting...

...knows how the physical kits are made and shipped; Chiara, the tech transfer expert who understands the somewhat...

... PubMed PMID: 8602510. 3. Yasutsugu Suzuki and Youichi Suzuki (2011). Gene Regulatable Lentiviral Vector...

...edits without any reagent-grade endangered Martian chicory root, but considering this is a needle in a haystack...

...Mark J., et al. "Evaluation of TCR gene editing achieved by TALENs, CRISPR/Cas9, and megaTAL nucleases....