Skip to main content
Addgene
Showing: 621 - 640 of 985 results

  1. Management for Scientists: What Makes a Good Manager Anyway?

    Type
    Blog Post
    ...the measure of work is not just time spent at the desk, but it is worth it. This requires a focus on deliverables...characteristics of scientists in the work place as described by Sebastiano Massaro in his Nature Biotechnology...

  2. Advice for Choosing a Research Project

    Type
    Blog Post
    ...good and bad data, and knowing how to follow the design, execute, analyze, repeat pathway that is required...critically before diving in. Research projects are designed to train graduate students and postdocs to think...

  3. Plasmids 101: Antibiotic Resistance Genes

    Type
    Blog Post
    ...when the cells are grown on selective media, and provides those bacteria with a pressure to keep your plasmid...gene and acts as a marker for the mutation. When designing these types of experiments it is best practice...

  4. Choosing a Good Mentor for Scientists

    Type
    Blog Post
    ...PDF at the end of this blog post. This document includes a list of questions to ask your potential advisors...some good pre-work you can find a lab that will deserve your hard work and an advisor that will be a lifelong...

  9. Using Addgene's New Viral Service for Your Research

    Type
    Blog Post
    ...the Addgene website. blog.addgene.org The blog provides tips for working with viruses including advice...on each viral prep’s specific material page and includes western blots as well as other expression data...

  10. Hot Plasmids and Viral Preps - September 2021

    Type
    Blog Post
    ...without end-point sequencing assays.  This tool was designed by putting together the coding sequence of a mCherry...MYC-CRISPR library targets E-boxes genome-wide. It was designed based on MYC-ChIP-seq data from several MYC-dependent...

  12. Another Pathway into Cells: iTOP

    Type
    Blog Post
    ...transduced using only protein. Read on for a description of the lab’s iTOP protein-only transduction method...transduction by osmocytosis and propanebetaine) to describe this method of protein transduction. Later studies...

  14. New and Upcoming Viral Vectors - June 2019

    Type
    Blog Post
    ...best serotype for their particular study, which includes comparing the transduction patterns of different...fluorescence of the sensor. This “contrast” variant provides improved detection for tracking large populations...

  15. Visualizing Genomic Loci with CRISPR-Sirius

    Type
    Blog Post
    ... chromosome-specific loci in the genome and the guides are relatively unstable.   Figure 1: CRISPRainbow... the gRNA tetraloop and went through subsequent design iterations to increase stability and minimize misfolding...

  17. The Importance of High Titer for AAV Transductions

    Type
    Blog Post
    ...if you end up concentrating your AAV above your desired titer and want to dilute it, err on the side of...less buffer than you would to achieve the exact desired concentration to avoid over-diluting, and make ...

  18. Learning Technical Skills at a Lab Central Workshop

    Type
    Blog Post
    ...form concatemers in later steps. This new NEB kit includes a step that both fragments the DNA and prevents...of the workshop, NEB gave a brief presentation describing the technology. Then we split into pairs to actually...

  19. Visualizing Translation at the Single Molecule Level

    Type
    Blog Post
    ... determine if proteins are targeted to their destination co- or post-translationally. TRICK: Visualizing... usually translated in a specific compartment. Despite these limitations, these techniques represent powerful...
Showing: 621 - 640 of 985 results