We narrowed to 775 results for: Dos

...plasmid tools that have come through our doors in 2015. Do you have a favorite new plasmid tool from the past... are turned on or off in a given area and the can do so in a very specific, precise manner. Previously... are bound to the coding region are displaced and do not rebind because they are transported back to the...their quest to understand lncRNAs: What can lncRNAs do by themselves? The Rinn lab has developed a novel...regulation has roles in many cellular functions, from endosomal trafficking to signaling pathways to the cell ...reticulum (ER), mitochondria, cytoskeleton, and endosomes. These fusion proteins can act as markers for ...Microtubules mCherry-alpha-tubulin Early Endosome Vacuolar Compartment mCherry-Rab5 BFP-Rab5 ...

... 2025. Catalog items are updated more frequently. Do you have suggestions for other plasmids that should...

... 2025. Catalog items are updated more frequently. Do you have suggestions for other plasmids that should...

...buffer as the one in the gel box (do not mix different buffers and do not use water). Microwave for 1-3...instructions on how to do this, visit the Gel Purification page. Tips and FAQ How do you get better resolution...min until the agarose is completely dissolved (but do not overboil the solution, as some of the buffer ...to the running buffer when you run the gel. If you do not add EtBr to the gel and running buffer, you will...the DNA in your sample lanes. For more details on doing diagnostic digests and how to interpret them please...typically used to separate 5 - 500 bp fragments. How do you get better separation of bands? If you have similarly...

...Integrative and conjugative elements (ICEs): what they do and how they work." Annual review of genetics 49 ...

...ancestral groups or, by extension, race. Many researchers do large-scale CRISPR screens in cancer cell lines, ...

...Cre-expressing neurons in a transgenic mouse line. By doing this, RABV expressing the viral protein EnvA on ...

...more about xCas9 - here’s what we know so far! Why do we need PAM flexibility? IDT estimates that there...

...it is essential to make sure that the selected FPs do not influence each other and thus potentially falsify...

...referred to as “instillation” or “gavage,” as they do not require any punctures. Intratracheal instillation...

...right? Non-coding RNAs (ncRNAs) are transcribed but do not encode proteins. They play important roles in...

... protein abundance. Finally, some degron tags can do more than just degrade! Read on to find out more....

...01756-1. PMID: 37095348. Breaking up is hard to do: Monomeric StayGold variants By Mike Lacy In 2022... it with an “assist peptide” that facilitates endosomal escape. Combining Cas9-PAGE with lentiviral sgRNA...based vectors (and vice versa), enabling repeat dosing. In a proof-of-concept study, mouse LY6A was delivered...

...electrical charges propagated through multiple cells to do things like translate perceived signals to physical...

...Additional protein tagging systems For those who do not work with C. elegans, several groups have designed...

... used the library to identify RBD mutations which do not interfere with ACE2 binding, but impact recognition...

...CRISPR-Cas system they block can be found here. How do anti-CRISPR proteins work? Anti-CRISPRs proteins ...

...views in this post are those of the guest blogger and do not necessarily represent the views of Addgene. Addgene...

...microbes to visualize host/pathogen interactions.   Do you have a favorite way to use GFP?  Comment and ...

...replication-competent lentivirus (RCL) in your target cells.  Q4: How do the packaging systems differ between 2nd- and 3rd-generation...