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We narrowed to 768 results for: Dos

Showing: 621 - 640 of 768 results

  1. What is Polymerase Chain Reaction (PCR)

    Type
    Protocol
    ...how to design primers for cloning purposes. What do I do if my PCR isn't working? Try adding 1µl of 25mM...36.8 μL Sterile dH 2 O (variable) Note: If you are doing multiple PCRs, save time by creating a “master mix...added to the master mix. Forward and Reverse Primers DO NOT get added to a master mix. Place reaction tubes...reverse primers to the thinned walled PCR tubes. Note: Do this before adding the master mix so you know that...according to the length of the target sequence. FAQ How do I design primers? See our reference page on how to...The ideal setup for this troubleshooting step is to do one reaction with each, and one reaction with both...templates. What does each ingredient specifically do? Template DNA: Contains the portion of DNA that we...

  2. CRISPR 101: Cytosine and Adenine Base Editors

    Type
    Blog Post
    ...to use the deaminated DNA strand as a template. To do so, they used a Cas nickase, instead of dCas9. The...editors often produce a mixed population of edits, ABEs do not display significant A to non-G conversion at ...

  3. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    ...plasmid tools that have come through our doors in 2015. Do you have a favorite new plasmid tool from the past... are turned on or off in a given area and the can do so in a very specific, precise manner. Previously... are bound to the coding region are displaced and do not rebind because they are transported back to the...their quest to understand lncRNAs: What can lncRNAs do by themselves? The Rinn lab has developed a novel...regulation has roles in many cellular functions, from endosomal trafficking to signaling pathways to the cell ...reticulum (ER), mitochondria, cytoskeleton, and endosomes. These fusion proteins can act as markers for ...Microtubules mCherry-alpha-tubulin Early Endosome Vacuolar Compartment mCherry-Rab5 BFP-Rab5 ...

  4. Plasmids for Stem Cell Research

    Type
    Collection
    ...more. Plasmid Gene/Insert Vector Type PI Publication Do you have suggestions for other plasmids that should...separate non-integrating episomes to allow sorting and dosage tracking of reprogramming factors Fluorescent tagged...

  5. Fluorescent Protein Guide: Subcellular Localization

    Type
    Collection
    ...Davidson Fluorescent Protein Collection . Return to top Do you have suggestions for other plasmids that should...14437 mRFP-Rab5 Early endosomes RAB5A mRFP Ari Helenius 61801 GFP-Rab4B Early endosomes Rab4B AcGFP Gia Voeltz...49201 mCh-Rab5 Early endosomes Rab5 mCherry Gia Voeltz 49147 BFP-Rab5 Early endosomes Rab5 BFP Gia Voeltz...GFP-Rab5B Early endosomes Rab5B AcGFP Gia Voeltz 79802 pTag-RFP-C-h-Rab5a-c-Myc Early endosomes Rab5a TagRFP...pTag-BFP-C-h-Rab5a-c-Myc Early endosomes Rab5a TagBFP James Johnson 13050 DsRed-Rab5 WT Early endosomes RAB5A DsRed2 Richard...GFP-EEA1 wt Early endosomes EEA1 GFP Silvia Corvera 42635 TagRFP-T-EEA1 Early endosomes EEA1 TagRFP-T Silvia...61804 mCh-Rab7A Late endosomes Rab7a mCherry Gia Voeltz 61803 GFP-Rab7A Late endosomes Rab7a AcGFP Gia Voeltz...

  10. Common Injection Routes in Mice

    Type
    Blog Post
    ...referred to as “instillation” or “gavage,” as they do not require any punctures. Intratracheal instillation...

  13. Plasmids 101: Degron Tags

    Type
    Blog Post
    ... protein abundance. Finally, some degron tags can do more than just degrade! Read on to find out more....

  14. Hot Plasmids February 2024

    Type
    Blog Post
    ...01756-1. PMID: 37095348. Breaking up is hard to do: Monomeric StayGold variants By Mike Lacy In 2022... it with an “assist peptide” that facilitates endosomal escape. Combining Cas9-PAGE with lentiviral sgRNA...based vectors (and vice versa), enabling repeat dosing. In a proof-of-concept study, mouse LY6A was delivered...

  15. Rabies and Neuronal Tracing

    Type
    Blog Post
    ...Cre-expressing neurons in a transgenic mouse line. By doing this, RABV expressing the viral protein EnvA on ...
Showing: 621 - 640 of 768 results