Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 770 results for: Dos;

Showing: 621 - 640 of 770 results

  1. Protocol - How to Run an Agarose Gel

    Type
    Protocol
    ...buffer as the one in the gel box (do not mix different buffers and do not use water). Microwave for 1-3...instructions on how to do this, visit the Gel Purification page. Tips and FAQ How do you get better resolution...min until the agarose is completely dissolved (but do not overboil the solution, as some of the buffer ...to the running buffer when you run the gel. If you do not add EtBr to the gel and running buffer, you will...the DNA in your sample lanes. For more details on doing diagnostic digests and how to interpret them please...typically used to separate 5 - 500 bp fragments. How do you get better separation of bands? If you have similarly...

  2. Bacterial Expression Systems

    Type
    Collection
    ...Return to top Content last reviewed on 17 June 2025. Do you have suggestions for other plasmids that should...

  4. CRISPR 101: Cytosine and Adenine Base Editors

    Type
    Blog Post
    ...to use the deaminated DNA strand as a template. To do so, they used a Cas nickase, instead of dCas9. The...editors often produce a mixed population of edits, ABEs do not display significant A to non-G conversion at ...

  5. What is Polymerase Chain Reaction (PCR)

    Type
    Protocol
    ...how to design primers for cloning purposes. What do I do if my PCR isn't working? Try adding 1µl of 25mM...36.8 μL Sterile dH 2 O (variable) Note: If you are doing multiple PCRs, save time by creating a “master mix...added to the master mix. Forward and Reverse Primers DO NOT get added to a master mix. Place reaction tubes...reverse primers to the thinned walled PCR tubes. Note: Do this before adding the master mix so you know that...according to the length of the target sequence. FAQ How do I design primers? See our reference page on how to...The ideal setup for this troubleshooting step is to do one reaction with each, and one reaction with both...templates. What does each ingredient specifically do? Template DNA: Contains the portion of DNA that we...

  6. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    ...plasmid tools that have come through our doors in 2015. Do you have a favorite new plasmid tool from the past... are turned on or off in a given area and the can do so in a very specific, precise manner. Previously... are bound to the coding region are displaced and do not rebind because they are transported back to the...their quest to understand lncRNAs: What can lncRNAs do by themselves? The Rinn lab has developed a novel...regulation has roles in many cellular functions, from endosomal trafficking to signaling pathways to the cell ...reticulum (ER), mitochondria, cytoskeleton, and endosomes. These fusion proteins can act as markers for ...Microtubules mCherry-alpha-tubulin Early Endosome Vacuolar Compartment mCherry-Rab5 BFP-Rab5 ...

  7. Rabies and Neuronal Tracing

    Type
    Blog Post
    ...Cre-expressing neurons in a transgenic mouse line. By doing this, RABV expressing the viral protein EnvA on ...

  14. Common Injection Routes in Mice

    Type
    Blog Post
    ...referred to as “instillation” or “gavage,” as they do not require any punctures. Intratracheal instillation...

  16. Hot Plasmids February 2024

    Type
    Blog Post
    ...01756-1. PMID: 37095348. Breaking up is hard to do: Monomeric StayGold variants By Mike Lacy In 2022... it with an “assist peptide” that facilitates endosomal escape. Combining Cas9-PAGE with lentiviral sgRNA...based vectors (and vice versa), enabling repeat dosing. In a proof-of-concept study, mouse LY6A was delivered...

  18. Plasmids 101: Degron Tags

    Type
    Blog Post
    ... protein abundance. Finally, some degron tags can do more than just degrade! Read on to find out more....
Showing: 621 - 640 of 770 results