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We narrowed to 982 results for: IGH@

Showing: 621 - 640 of 982 results

  1. Scientific Sharing in the Time of COVID-19: Databases and Resources

    Type
    Blog Post
    ...within the scientific community The team behind Crowdfight COVID-19 strives to match up scientists across...with different technical knowledge that could help fight COVID-19. They’ve also collated a list of useful...  "We realized that there is now a huge pool of highly skilled scientists, willing to volunteer their ...

  2. What's New in CRISPR - March 2020

    Type
    Blog Post
    ...In this quarterly blog series, we’ll highlight a few of the new CRISPR plasmids available at Addgene....that controls nuclear localization (LINuS). Blue light renders the NLS accessible and the complex enters...

  4. What's New in CRISPR - September 2019

    Type
    Blog Post
    ...In this quarterly blog series, we’ll highlight a few of the new CRISPR plasmids available at Addgene....and human cells. Using the structure of CasX at eight different states of assembly from cryo-electron ...

  5. SpyLigase Irreversibly Locks Peptides Together for Efficient Cell Capture

    Type
    Blog Post
    ...efficient capture. We wanted to be able to build a highly multivalent assembly connected by covalent bonds...St. Andrews discovered the domain. It is small, highly stable and has this amazing chemistry locking the... the variation in cancer, not just the CTCs with high marker expression level. Labs have been trying different...

  6. The Michael Davidson Collection: One-Stop Shop for Fluorescent Proteins

    Type
    Blog Post
    ... has been the go-to place. In 2012, his National High Magnetic Field Lab worked with an impressive 1,350... Proteins 101: GFP Fusion Proteins - Making the Right Connection.  mEmerald plasmids and many more! Baird...know how all the parts go together, we can fix it. Right now, we have no idea." Now, it will be that much...

  7. Lentiviral CRISPR Libraries Enable Genome-Scale, Knockout Screening

    Type
    Blog Post
    ...Cas9, then with the sgRNAs, and can result in a higher titer due to the smaller size of the plasmids. ...of selection. Shalem and Sanjana say they kept a high representation of library within cells and aimed... construct, but in some biological systems one “might need larger numbers.” Further details and protocols...

  8. Embracing Serendipity: A Crucial Element in the PhD Journey

    Type
    Blog Post
    ...knowledge base extensively, you open doors to fresh insights and broaden your understanding, ultimately enhancing...areas in need of improvement. These unrelated ideas might hold the key to unlocking new perspectives or even...to leave a little space for the unexpected — it might just be the key to unlocking the next frontier of...

  9. Understanding GPCR Signaling Using TRUPATH

    Type
    Blog Post
    ... in signaling processes including smell, taste, sight, inflammation, and neurotransmission. But with approximately...activated downstream of GPCRs that are not involved in sight (Olsen RHJ et al., 2020). A transducer complex consists...Gγ-GFP2 will be in close association, resulting in a high level of GFP2 emission. Following activation in ...

  10. What's the Best Way to Elute and Store Your Plasmid DNA?

    Type
    Blog Post
    ...protocol, you might have noticed that there are many options to elute your DNA prep. You might see protocols...denature, break, and even change the DNA sequence. High pH favors the breakage of the hydrogen bonds, destabilizing...

  11. CRISPR 101: Cas9 Nickase Design and Homology Directed Repair

    Type
    Blog Post
    ... these results, it’s clear that editing is much higher in the PAM-out configuration. In addition, D10A...D10A editing efficiency is consistently higher than that of H840A, especially with smaller nick distances...cuts close to your insertion site, you can’t obtain high HDR efficiency. Nickases create a staggered cut ...

  12. CUT&RUN: An Improved Method for Studying Protein-DNA Interactions

    Type
    Blog Post
    ... Hill. Chromatin immunoprecipitation followed by high-throughput sequencing, ChIP-Seq, is the go-to method... purified for subsequent library preparation and high-throughput sequencing. Improvements to the CUT&RUN...challenging to collect large numbers of cells or when a high degree of signal to noise is desired. It also provides...

  13. Bricking Science: Portraying Scientific Reality Through LEGO

    Type
    Blog Post
    ... LEGO and science: The potential is endless One night, while opening a LEGO set with my husband, I thought...that resembles a real laboratory. It only took one night to build the first model, but because we are [email protected]. Who knows, your story might just get “legofied.” Many thanks to our guest blogger...

  16. Using Ultrasound to Image Bacteria in vivo: Acoustic Reporter Genes

    Type
    Blog Post
    ... available, and have deep tissue penetration and high spatial resolution. By piecing together genes from...are sequentially exposed to acoustic pulses with high enough amplitudes to first collapse arg2 and then...development of ARGs and have already developed a high-throughput screening method for serial acoustic ...

  17. Networking and Inspiring Young Scientists at Freshers Fairs

    Type
    Blog Post
    ...Chemistry symposium and a career night. The society also visits high schools to promote chemistry degrees...scientific meetings for students to attend. It also hosts high level academic debates for its members to become...

  18. Addgene @ Keystone: Thoughts on Precision Genome Engineering and Synbio

    Type
    Blog Post
    ...arrive. Getting off the plane in Bozeman Sunday night, it was easy to spot meeting participants. They ...the ones holding poster tubes (or as our baffled flight attendant called them, "long, skinny things") and...thinking I was an engineer. The talks we heard Monday night represented engineering first and foremost, from...

  19. Management for Scientists: What Makes a Good Manager Anyway?

    Type
    Blog Post
    ... allow his team to work mostly at home. Having a high degree of freedom and diversity built into their...new things, diversity of responsibilities, which might include training or teaching others, research, and...sometimes taken to an extreme by scientists) Are highly mobile and can move to a new workplace if opportunities...

  20. 10 Ways to Share Your Science!

    Type
    Blog Post
    ...telling it another way. You’ll know you are on the right track if they start asking you questions. Remember...Escape from the Ivory Tower.”  9)   If you think you might want to get serious about science writing, look ...News. Ask your institutional news office if there might be opportunities to blog or write for them. 10)  ...
Showing: 621 - 640 of 982 results