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We narrowed to 971 results for: LARS

Showing: 621 - 640 of 971 results

  1. Four Factors that Differentiate the Stem Cell Field

    Type
    Blog Post
    ...affected the stem cell community – a community largely created due to Yamanaka’s seminal work. It didn't... prominent role in the scientific endeavor – particularly in fields like stem cell biology where questions...about his clinical trial patients with advanced macular degeneration; Laurenz Studer discussed his work...

  2. Management for Scientists: Delegating is Key

    Type
    Blog Post
    ... the heart of delegating for success.  Meet regularly before during and after the assignment to discuss...review project progress. I recommend putting a regular check-in meeting on the calendar even if it is ... Resources on the Addgene Website Are You a Molecular Biologist? Find Plasmids for Your Research Here...

  3. Resume Writing for Non-academic Science Careers

    Type
    Blog Post
    ... data, then the chance is that you have handled large data sets and are familiar with statistical analysis...of that, perhaps you were involved in some extracurricular activities – did you volunteer to do science..., many universities make their job postings and salary expectations public, so start by looking at these...

  4. Advice for Scientists Starting a Lab

    Type
    Blog Post
    ...strong collaborative research team motivated by similar visions and goals? We continue our conversation... time in his office, he gradually incorporated regular group meetings, journal clubs, and focused sub-...and a friend. George Church runs his lab fairly similarly to his advisor Wally Gilbert: “Very interdisciplinary...

  5. Finding and Joining Your Dream Lab

    Type
    Blog Post
    ...engagement and access to growth opportunities, not salary and benefits. Therefore, lab selection should focus...Alternatively, perhaps the lab publishes and presents regularly with labs around the world. You may prefer a more... making this choice.  How big is the lab? While larger labs tend to be more "chaotic" and offer less micromanaging...

  6. Plasmids 101: Mammalian Vectors

    Type
    Blog Post
    ...can either express the plasmid DNA transiently (similar to bacteria) or incorporate the genetic material...virus (EBV) nuclear antigen 1 (EBNA1) or the SV40 large-T antigen (293E or 293T cells), allow for episomal.... Positive selection in mammalian cells works similarly to that in bacteria and a table of the most commonly...

  7. Top Requested Lentivirus and AAV of 2016

    Type
    Blog Post
    ...used to investigate the effects of activating particular signaling pathways in specific cell types (typically...typically neurons). While all of these viruses are popular, pAAV-hSyn-DIO-hM4D(Gi)-mCherry contains a double...animals, where cre expression is restricted to a particular cell type. The human synapsin promoter further...

  8. A Quick Guide to a Career in Software Product Management

    Type
    Blog Post
    ...could code the best operations software possible. Similarly, when the developers needed to know information...relationship-building and influence. You’ll need a large deal of influence and strong relationship-building...Technologies for the North America region. She is particularly interested in DNA sequencing tech, and the intersection...

  9. New and Upcoming Viral Vectors - May 2020

    Type
    Blog Post
    ...noninvasive methods for manipulating cell activity, particularly in the brain, and holds promise in the development...development of future therapies. Our popular plasmid encoding a Cre dependent version of the DREADD hM3D ...on availability date or to be notified when a particular catalog item is available for order please email...

  10. Fluorescent CRISPR Reporters: SRIRACCHA and GEmCherry2

    Type
    Blog Post
    ...cell type? You can use DNA sequencing or other molecular cloning techniques to determine CRISPR/sgRNA efficiency...site in the genome you are trying to target play a large role in Cas9 activity and efficiency (Sander and... structure and genomic recognition sites play a large role in CRISPR-Cas9 efficiency. They continued to...

  12. Hot Plasmids - May 2022

    Type
    Blog Post
    ...offset due to the nanobody-GFP interaction). The modularity of the system provides researchers better flexibility...comparable to commonly used CRISPR/Cas9 methods for large inserts. However, this system has a much lower error... a fluorescent protein coupled to a specific subcellular localization peptide. By combining three different...

  13. Antibodies 101: ChIP

    Type
    Blog Post
    ...for either tissues or cells. The process is very similar for both, though tissue samples do require an extra...thinking, well, these are mostly fairly standard molecular and cell biology techniques. What’s all the fuss...www.wannapik.com/   Even though ChIP requires a larger upfront investment than many other antibody applications...

  14. Antibodies 101: Flow Cytometry Controls

    Type
    Blog Post
    ...unsure what your population will look like for a particular marker, you can use the reference plots provided...isotype controls An isotype sample is prepared very similarly to a FMO sample: you make a sample from a combined...and then add in your isotype control for that particular marker. This enables you to identify any non-...

  15. Viral Vectors 101: The AAV Retrograde Serotype

    Type
    Blog Post
    ...Fig. 1B). Tracers are typically delivered to a particular location and they either trace downstream from..., you can map what regions are signaling to a particular site while also activating or inhibiting gene...these vectors can exhibit spurious expression, particularly at the injection site (Fischer, KB. 2019). The...

  16. The Many Reasons for Irreproducible Research- and a Vaccine to Eradicate It

    Type
    Blog Post
    ...and not just the subset of those that return a particular result. Anything else that occurs during the ...are known. The author submits the research plan (similar to a preregistration, but usually with a fully ...Promotion Guidelines; preregistration to increase clarity in study design and analysis; removing publication...

  17. Choosing a CRISPR Nuclease: Site Accessibility, Specificity, and Sensitivity

    Type
    Blog Post
    ...NG (Nishimasu et al., 2018). The David Liu Lab similarly described xCas9, which displayed the ability to... (HypaCas9). This nuclease showed decreased or similar off-target activity, relative to eSpCas9 and SpCas9...degree of both specificity and sensitivity across a large number of loci and gRNA lengths. Recent alternatives...

  18. A Novice's Perspective on Neuroscience - SfN2017

    Type
    Blog Post
    ... almost all of the attendees were going to be molecular biologists. This was not at all the case. The ...neurobiology (and really in all of biology) require large data sets. Whether these data sets include videos... make the virus for you. Neuroscientists are particularly interested in using AAVs because they provide...

  19. Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

    Type
    Blog Post
    ..., gRNA and pol III terminator. These relatively large cassettes (considering the gRNA itself is ~100 bases...advantageous to instead use a preexisting, conserved cellular machinery to process gRNAs. Hijacking tRNA processing...frequency of both indels at each locus, as well as large deletions. They detected fragment deletion with ...

  20. How to Make Friends and Meet People at a Scientific Conference

    Type
    Blog Post
    ...national recognition and the more people you know, the larger your profile in the community Job hunting outside...thing to consider is the size of the conference. Large conferences have many opportunities but can be overwhelming... overwhelming as far as meeting new people. At large conferences, look for smaller events or meet-ups ...
Showing: 621 - 640 of 971 results