...be covering this ever-versatile fluorophore in a later post, so stay tuned! Transient transfection and ...transfection A stable transfection is used to create a population of cells that have fully and successfully incorporated... COS-1, 293HEK Inhibits termination step of translation 2-10 ug/mL G418/Geneticin neo HeLa, NIH3T3,...

..., integrated reporter for assaying CRISPR/Cas-stimulated HDR activity) The Madison lab created SRIRACCHA...Joung JK (2014) CRISPR-Cas systems for editing, regulating and targeting genomes. Nature Biotechnology 32...integrated surrogate reporter for assaying CRISPR/Cas9-stimulated homology-directed repair. Journal of Biological...

...contained cold tolerant chaperones (the Cpn 10/60 isolated from Oleispira antarctica, and the P. haloplanktis...psychrophilic systems as well as engineering and regulating psychrophilic recombinant proteins.  To infinity...2019 All-India iGEM Meet-up, which served as a platform for all the Indian iGEM teams to interact with...

...lab or pivot to a managerial role. For those contemplating the leap into management, the journey can be...being a great scientist doesn’t automatically translate to being an effective manager. Mismanaging (no... Kim Scott Courses: Leadership programs on platforms like Coursera, LinkedIn Learning, or edX. Podcasts...

...hepatitis virus post‐transcriptional regulatory element; sequence that stimulates the expression of transgenes...-retroviral plasmids have similar names? The nomenclature of the plasmid backbone is meant to be descriptive...

...plasmids for expression of mAbs, the Trimmer lab isolated RNA from cryopreserved hybridomas, immortalized...then used to generate cDNA, which  was used as a template for PCR amplification with a degenerate primer...grown in tissue culture, they were also able to isolate RNA from non-recoverable hybridoma stocks, without...

...miniature CRISPR-Cas system for mammalian genome regulation and editing Anti-FLAG Frankenbodies for modular... genes, researchers at the Genetic Pertubation Platform at the Broad Institute have expanded the genetic...miniature CRISPR-Cas system for mammalian genome regulation and editing by: Rachel Leeson The large size ...

...scientific community through Addgene’s sharing platform. Expanding further beyond the US and increasing...breakthrough and innovation. With any kind of open platform, there is the potential for increased speed and...sector in both patent law and general life science regulatory law. My role at Suffolk is to help create new...

...scientific and medical tools. As scientists, we can manipulate what this cargo is and where it goes, turning...mutagenesis in the host. Adenoviruses can deliver relatively large cargo inserts (>8 kb). Adeno-associated...their low immunogenicity, which means they do not stimulate a strong immune response when introduced into ...

...cellular extracts to perform transcription and translation in vitro, and the new BioBuilder module allows... mentors, and provides a nationally recognized platform in March to exhibit projects at any stage of completion...breakdown, production of fire-resistant housing insulation, and cleaning up salt runoff from winter icing.BioBuilderClub...

...sequence (NGG, etc.) as well as their orientation relative to the gRNA (5’ or 3’). In some cases, there is... used by Jinek et al. 2012, or browsing for the latest AsCas12a plasmids, Addgene and CasPEDIA have got...to browse entries by the current phylogenetic nomenclature and discover orthologs with similar properties...

...certain to choose your recommenders based on your relationships to the person as well as their current sphere...just as you would for an in-person interview. Formulate and practice a set of common questions with a ...academic resources among economically disadvantaged populations. Roo is the co-founder of STEAMid.       Rose...

...effort to track down every last one of those gene regulatory proteins and the places in the genome where they...closely together on several projects and this is one related to gene expression. One goal of the ENCODE project...resistance to neomycin so that you can select the population of cells with the tagged transcription factor...

...community. As a result, I have occasionally pondered translating my research into a startup company. However, ...effort in preparation and practice is directly related to success presenting." A journey from graduate...LabSpaces Blog Find a Buisness Plan Competition Good Relations - PR for the Biotech Business - Posted on Nature.com...

... the cell with a mutant template and fooling the cell into using that template for repair. By using these...the two ends back together or using a homologous template provided by the sister chromosome. As cells take...

...amplitude, wavelength sensitivity, and off-kinetics correlated with the model’s predictions.  Three top performers...reach the VTA. Mice were then allowed to “self-stimulate” their VTA dopaminergic neurons by triggering ...H134R) expressing mice did not have this self-stimulation response. This result showed that ChRger2 stronger...

...pathway is regulated. You’ve read all the background papers you could get your hands on, formulated a hypothesis...immunoprecipitation-mass spectrometry strategies that isolate an antibody’s immune complexes and confirm interacting...

...available at Addgene. If you want to read more content related to CRISPR technology, subscribe to our CRISPR blog... plasmids Enhanced prime editing systems by manipulating MMR machinery In their Cell paper, the Liu Lab...other prime editing tools, the desired edits are templated by the 3′ extension of the pegRNAs. PEDAR is capable...

...expression cassettes on a single plasmid (Grefen and Blatt, 2012). Similar to traditional Gateway™ cloning,...Fluorescence Complementation (rBiFC, Grefen and Blatt, 2012). The 2in1 Plasmid Toolkit consists of 4 plasmid...Biol, 2018. PubMed PMID: 29043675.Grefen C and MR Blatt. Biotechniques 2012. PubMed PMID: 23066669.Hecker...

...string together all the words that I could remember related to this paper and/or biology in general until I... turn your slide deck into a chalk talk. Your translator doesn’t know the term “diving board” so you end...or talk extremely quickly. You realize, much too late, that you labeled your gene of interest incorrectly...