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We narrowed to 876 results for: NSI;

Showing: 621 - 640 of 876 results

  1. AAV ddPCR Titration

    Type
    Protocol
    ... 10% bleach. Safety Warnings AAV is generally considered biosafety level 1 but may require BSL-2 handling...45° angle. Count to 20 while slowly and gently dispensing the droplets down the side of the tube. Place... 95 10 2 1 Denaturation 95 0.5 2 50 Annealing/Extension 60 1 2 50 Signal Stabilization 98 10 2 1 Hold ... should be close to zero (B08). At Addgene, we consider runs with an NTC > 10 to be invalid. To reduce...

  3. CRISPR 101: RNA Editing with Cas13

    Type
    Blog Post
    ...enzymes do not contain the RuvC and HNH domains responsible for DNA cleavage, so they cannot directly edit.... PspCas13b does not require a PFS, and it is sensitive to mismatches in target RNA from bases 12-26 of...

  5. Droplet Digital PCR for AAV Quantitation

    Type
    Blog Post
    ...For these reasons, we are in the process of transitioning our titering method from qPCR to ddPCR. Droplet...titer your AAV by ddPCR. Droplet digital PCR is a sensitive assay, so cross contamination into a no template...

  7. Tips for arabidopsis transformation

    Type
    Blog Post
    ...that is wide enough to dunk your plant’s flowers inside. 50 mL falcon tubes or plastic weigh boats are ...from a colony on a plate will reach an optimal density in 18-24 hours. I then add 0.5 mL of this starter...

  8. Course-Based Undergraduate Research Experiences (CUREs)

    Type
    Blog Post
    ...students. But he also found that his teaching responsibilities left him with limited time for one-on-one ...notes the importance of acknowledging student apprehensions about “failure” (protocols not working, hypotheses...

  9. Uncovering Ancestral Bias in CRISPR Research

    Type
    Blog Post
    ...connections between biology and ancestral groups or, by extension, race. Many researchers do large-scale CRISPR ...potential for bias but to actively look for and consider bias as a confounding factor when interpreting...

  11. Lentiviral Vector Uses and Overview

    Type
    Blog Post
    ...non-dividing cells. The first lentiviral vector system consisted of three plasmids: the packaging, envelope, and...capacity of a lentiviral vector but for which transient transgene expression is sufficient. Lentiviral...

  12. Tips for Screening with Yeast Two Hybrid Systems

    Type
    Blog Post
    ...Saccharomyces cerevisiae in 1989 and are still used extensively to screen for molecular interactions in the cell...post-translationally modified, co-expression of the enzyme responsible for the modification in the assay host strain...

  15. AAV Production in HEK293 Cells

    Type
    Protocol
    ... through a 0.22 μm membrane. Store at 4 °C. Considerations Before You Start The health of the HEK293T ...vigorously multiple times to obtain a single cell suspension (no clumps of cells). Pool cells from 2 x T-175...forth or shake vigorously to obtain a single cell suspension (no clumps). Count cells using a hemocytometer...above. Add 50 units of benzonase per mL of viral suspension (you should have ~18 mL from steps 12 & 13). ...

  16. Analyzing CRISPR Editing Results with ICE from Synthego

    Type
    Blog Post
    ...editing field by making experimental workflows considerably easier, faster, and more efficient than previous...novice to expert-level researchers in achieving consistently successful CRISPR experiments. Jessica is a ...

  17. Hot Plasmids - August 2020

    Type
    Blog Post
    ...CRISPR/Cas Toolkit for plants includes 95 plasmids consisting of CRISPR/Cas nucleases, base editors, gRNA backbones...activation-based norepinephrine (GRABNE) sensors allow for sensitive and high spatiotemporal measurements of norepinephrine...

  18. Plasmids 101: Plasmid Incompatibility

    Type
    Blog Post
    ...partitioning system in the bacteria, but generally it consists of a centromere-like region on the plasmid, a ...2015) Imaging centromere-based incompatibilities: Insights into the mechanism of incompatibility mediated...

  19. Mesothelioma - Causes, Symptoms, and Treatment

    Type
    Blog Post
    ...from many other types of cancer. Mesothelioma is considered an orphan cancer; it is rare disease with only...non-resectable mesothelioma by the National Comprehensive Cancer Network, a coalition of the top 27 cancer...

  20. Transferable Skills Guide: Creativity

    Type
    Blog Post
    ...with idea generation, try this thought exercise: Consider the issue as if there were no restraints on your...something completely unrelated to the issue and consider how it could be shaped to solve your problem. ...
Showing: 621 - 640 of 876 results