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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 909 results for: Nes;

Showing: 621 - 640 of 909 results

  1. Single-cell tracking of lineage and identity with CellTag

    Type
    Blog Post
    ...partially reprogrammed and re-expressed fibroblast genes. Most cells ended up taking the ‘dead-end’ path....CellTag is a combinatory cell-indexing tool that combines scRNA-seq and lineage tracking to track the dynamics...

  3. New and Upcoming Viral Vectors - Spring 2019

    Type
    Blog Post
    ...with different properties (for example, overall brightness or on/off kinetics) that makes them suitable ...pAAV.hSyn-FLEX.iGABASnFR) has good membrane localization and brightness. The F102G variant (pAAV.hSyn-FLEX.iGABASnFR....

  4. Comparing Cas9 to NgAgo: Can the Argonautes Best CRISPR?

    Type
    Blog Post
    ... and genomic DNA in a variety of mammalian cell lines, with an efficiency similar to that of Cas9. Like...certain guide sequences - using 5 guides/gene for 10 genes, they did not observe differences in cleavage efficiency...

  5. Addgene Begins Distribution of Recombinant Antibodies

    Type
    Blog Post
    ...derived were tested for specificity in knock-out lines or animals.  Addgene will continue to add more antibodies...antibodies, for neuroscience research and other disciplines, over time. The resource may also expand to include...

  6. RUBY-Red Siliques

    Type
    Blog Post
    ...fluorescent proteins. Selection using resistance genes usually involves killing the untransformed seedlings...then green offspring can be selected instead of red ones. Other innovative uses of RUBY are possible, for...

  8. Cultivating Community Science at BosLab

    Type
    Blog Post
    ... with audiovisual media, software engineering, business consulting, mathematics, and more. That’s right...BosLab. Once he optimizes plant cell culture and transgenesis he wants to share his knowledge with the community...

  10. Visualizing Genomic Loci with CRISPR-Sirius

    Type
    Blog Post
    ...the gRNA target site. In addition to increased brightness, they also found that the signal to noise ratio... observing low-copy genomic loci or single copy genes. If you want to visualize genomic loci brightly,...

  11. 5 Great Apps for Lab Life

    Type
    Blog Post
    ...smartphone to help me with my research in terms of preparedness, organization, and sharing data. Here are five...Stacey! Sean D. Stacey is a Ph.D. student at East Tennessee State University. He works with gene expression...

  12. What Good Citizenship Can Do for Reproducibility in Science

    Type
    Blog Post
    ...going forward. This is not just a question of being honest and straightforward, it’s an important part of ... various different versions of checklists and guidelines to help authors understand what they are expected...

  13. Networking Follow-up: I Met a Person. Now What?

    Type
    Blog Post
    ...my guess is that people will not be exchanging business cards for awhile or ever so your LinkedIn profile...She supported me and answered my questions and honestly, she was the only person who really understood...

  14. Predicting Adverse Reactions to Monoclonal Antibody Drugs

    Type
    Blog Post
    ...performed using traditional 2D assays with immune cell lines or peripheral blood mononuclear cells (PBMC) in .... P., Herskovitz, J., Goletz, T. J., Zhou, L., Moxness, M., Flynn, G. C., Narhi, L. O., & Jawa, V. (2016...

  15. Trading Spaces - Visiting Scientist Edition

    Type
    Blog Post
    ... know that science usually doesn’t follow our timelines! Prepare a research plan to set yourself up for...applying to a structured program, they likely have guidelines to consult on this topic.   Etiquette...

  16. K. phaffii: Rising to the Occasion

    Type
    Blog Post
    ...experimental model. In fact, approximately 25% of yeast genes have a human counterpart, enabling study of human-relevant...the strain tractable for peroxisomal studies on biogenesis, degradation, and proliferation. Genome manipulation...

  18. 25 Work From Home Tips for Scientists

    Type
    Blog Post
    ... help you prepare for sleep. At Addgene, our mindfulness group is sticking with their Headspace practice...19. You can also give back by supporting local businesses (ex: purchasing a gift card to use later or ordering...

  19. Optimizing Donor DNA for Enhanced CRISPR Genome Editing

    Type
    Blog Post
    ...disrupt target gene sequences (thus inactivating genes), while breaks repaired by homology directed repair... which sequence replacement holds some kind of fitness advantage but error-prone repair of the gene would...

  20. Components of CRISPR/Cas9

    Type
    Blog Post
    ...Variant of Cas9 PAM Sequence Streptococcus pyogenes (SP); SpCas9 3' NGG SpCas9 D1135E variant...sites with the aid of two RNAs: the crRNA which defines the genomic target for Cas9, and the tracrRNA which...
Showing: 621 - 640 of 909 results